{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T07:33:44Z","timestamp":1781249624374,"version":"3.54.1"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Project of the Ministry of Public Security","award":["2022JSZ10"],"award-info":[{"award-number":["2022JSZ10"]}]},{"name":"Key Project of the Ministry of Public Security","award":["2022BZ126"],"award-info":[{"award-number":["2022BZ126"]}]},{"name":"Industry Standard Search Dog Field Search Use Specification","award":["2022JSZ10"],"award-info":[{"award-number":["2022JSZ10"]}]},{"name":"Industry Standard Search Dog Field Search Use Specification","award":["2022BZ126"],"award-info":[{"award-number":["2022BZ126"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Renal failure is a common chronic disease in dogs that substantially affects both their quality of life and longevity. The objective of this study was to assess the protective mechanisms of baicalin in cisplatin-induced Madin\u2013Darby canine kidney (MDCK) epithelial cells\u2019 apoptosis model and explore the impacts of baicalin at varying doses on various indexes, such as cisplatin-induced MDCK cell apoptosis, oxidation and antioxidation, and inflammatory factors. (Methods) MDCK cells in the logarithmic growth phase were randomly divided into a control group, a model group (20 \u03bcmol\/L cisplatin), and a baicalin-protection group (20 \u03bcmol\/L cisplatin + 50, 25 \u03bcmol\/L baicalin) and received the corresponding treatments for 24 h. The effects of cisplatin on MDCK cell apoptosis, oxidation and antioxidation, inflammatory factors, and other indicators were studied, and the relieving effect of baicalin on cisplatin-induced MDCK cell damage was explored. Calcein\/PI staining and Annexin V-FITC\/PI staining showed that cisplatin induced the apoptosis of MDCK cells, while baicalin effectively reduced the damage caused by cisplatin. The ELISA results demonstrated a significant elevation in the nitric oxide (NO) and malondialdehyde (MDA) levels within the MDCK cells following treatment with cisplatin (p &lt; 0.01). In addition, superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT) activities remarkably declined (p &lt; 0.01), while tumor necrosis factor \u03b1 (TNF-\u03b1), interleukin-1\u03b2 (IL-1\u03b2), and interleukin-6 (IL-6) expression within the MDCK cells were apparently elevated (p &lt; 0.01). However, baicalin treatment resulted in opposite changes in these factors. The findings suggested that baicalin exhibits potential in mitigating cisplatin-induced oxidative stress and inflammation in MDCK cells. As revealed with the Western blot results, cisplatin promoted P62, P53, and BAX protein levels, increased mTOR phosphorylation, inhibited AMPK phosphorylation, and reduced Beclin1 and BCL-2 protein levels. However, a contrasting trend was observed following baicalin treatment. Cisplatin can inhibit the activity of MDCK cells, lead to abnormalities in oxidation and antioxidation functions and cell inflammatory factors, and accelerate cell apoptosis. Moreover, baicalin can significantly alleviate the damage of cisplatin to MDCK cells.<\/jats:p>","DOI":"10.3390\/metabo13121173","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T10:10:39Z","timestamp":1700820639000},"page":"1173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells"],"prefix":"10.3390","volume":"13","author":[{"given":"Yao","family":"Wang","sequence":"first","affiliation":[{"name":"College of Police Dog Technology, Criminal Investigation Police University of China, Shenyang 110854, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanguo","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Police Dog Technology, Criminal Investigation Police University of China, Shenyang 110854, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuwei","family":"Xie","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Shenyang Medical College, Shenyang 110031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Shenyang Medical College, Shenyang 110031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.3389\/fvets.2020.00293","article-title":"Pet Ownership Patterns and Successful Aging Outcomes in Community Dwelling Older Adults","volume":"7","author":"Friedmann","year":"2020","journal-title":"Front. 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