{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:55:21Z","timestamp":1787320521365,"version":"build-2736575974"},"reference-count":86,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T00:00:00Z","timestamp":1707609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Health Commission of Guangdong Province in China","award":["A2021195"],"award-info":[{"award-number":["A2021195"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Long-term exposure to noise can cause irreversible hearing loss. Considering that there is no effective drug treatment, it is important to seek preventive treatment for noise-induced hearing loss (NIHL). Although astragaloside IV (AS-IV) protects against NIHL by reducing serum inflammatory factors, there is scarce information on the regulation of inflammatory factors by AS-IV to prevent NIHL. We investigated the hearing thresholds and relationship between the serum levels of inflammatory cytokines and intestinal microbiota of c57bl\/6j mice exposed to noise (103 dB SPL 4 h\u00b7d\u22121) for 7 days, treated with or without AS-IV. Our results revealed a lower hearing threshold and lower serum levels of TNF-\u03b1, TNF-\u03b3, IL-6, IL-1\u03b2, and IFN-\u03b3 in the mice treated with AS-IV. Additionally, AS-IV increased the abundance levels of the phylum Firmicutes, class Bacillus, order Lactobacillus, and family Lactobacillus (p &lt; 0.05), and decreased those of the phylum Bacteroidetes and order Bacteroidales (p &lt; 0.05). Lactobacillus and Bacilli negatively correlated with TNF-\u03b1, TNF-\u03b3, and IL-1\u03b2; Erysipelotrichaceae negatively correlated with INF-\u03b3; and Clostridiales positively correlated with IL-1\u03b2. In conclusion, AS-IV reduces the elevation of hearing thresholds in mice, preventing hearing loss in mice exposed to noise, and under the intervention of AS-IV, changes in the levels of inflammatory factors correlate with intestinal flora. We suggest that AS-IV improves intestinal flora and reduces inflammation levels in c57bl\/6j mice exposed to noise.<\/jats:p>","DOI":"10.3390\/metabo14020122","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T03:50:27Z","timestamp":1707709827000},"page":"122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Astragaloside IV on Hearing, Inflammatory Factors, and Intestinal Flora in Mice Exposed to Noise"],"prefix":"10.3390","volume":"14","author":[{"given":"Junyi","family":"Li","sequence":"first","affiliation":[{"name":"School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Natarajan, N., Batts, S., and Stankovic, K.M. 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