{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:28:51Z","timestamp":1791498531167,"version":"4.3.4"},"reference-count":112,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nutrition Science Initiative","award":["R01DK125701"],"award-info":[{"award-number":["R01DK125701"]}]},{"name":"Nutrition Science Initiative","award":["R01NR019083"],"award-info":[{"award-number":["R01NR019083"]}]},{"name":"Nutrition Science Initiative","award":["UL1TR001442"],"award-info":[{"award-number":["UL1TR001442"]}]},{"name":"Nutrition Science Initiative","award":["UL1TR002378"],"award-info":[{"award-number":["UL1TR002378"]}]},{"name":"Nutrition Science Initiative","award":["RRID:SCR_023527"],"award-info":[{"award-number":["RRID:SCR_023527"]}]},{"name":"University of California San Diego Altman Clinical and Translational Research Institute","award":["R01DK125701"],"award-info":[{"award-number":["R01DK125701"]}]},{"name":"University of California San Diego Altman Clinical and Translational Research Institute","award":["R01NR019083"],"award-info":[{"award-number":["R01NR019083"]}]},{"name":"University of California San Diego Altman Clinical and Translational Research Institute","award":["UL1TR001442"],"award-info":[{"award-number":["UL1TR001442"]}]},{"name":"University of California San Diego Altman Clinical and Translational Research Institute","award":["UL1TR002378"],"award-info":[{"award-number":["UL1TR002378"]}]},{"name":"University of California San Diego Altman Clinical and Translational Research Institute","award":["RRID:SCR_023527"],"award-info":[{"award-number":["RRID:SCR_023527"]}]},{"name":"Georgia Clinical Translational Science Alliance","award":["R01DK125701"],"award-info":[{"award-number":["R01DK125701"]}]},{"name":"Georgia Clinical Translational Science Alliance","award":["R01NR019083"],"award-info":[{"award-number":["R01NR019083"]}]},{"name":"Georgia Clinical Translational Science Alliance","award":["UL1TR001442"],"award-info":[{"award-number":["UL1TR001442"]}]},{"name":"Georgia Clinical Translational Science Alliance","award":["UL1TR002378"],"award-info":[{"award-number":["UL1TR002378"]}]},{"name":"Georgia Clinical Translational Science Alliance","award":["RRID:SCR_023527"],"award-info":[{"award-number":["RRID:SCR_023527"]}]},{"name":"Emory University Integrated Metabolomics and Lipidomics Core Facility","award":["R01DK125701"],"award-info":[{"award-number":["R01DK125701"]}]},{"name":"Emory University Integrated Metabolomics and Lipidomics Core Facility","award":["R01NR019083"],"award-info":[{"award-number":["R01NR019083"]}]},{"name":"Emory University Integrated Metabolomics and Lipidomics Core Facility","award":["UL1TR001442"],"award-info":[{"award-number":["UL1TR001442"]}]},{"name":"Emory University Integrated Metabolomics and Lipidomics Core Facility","award":["UL1TR002378"],"award-info":[{"award-number":["UL1TR002378"]}]},{"name":"Emory University Integrated Metabolomics and Lipidomics Core Facility","award":["RRID:SCR_023527"],"award-info":[{"award-number":["RRID:SCR_023527"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Little is known about lipid changes that occur in the setting of metabolic-dysfunction-associated steatotic liver disease (MASLD) regression. We previously reported improvements in hepatic steatosis, de novo lipogenesis (DNL), and metabolomic profiles associated with oxidative stress, inflammation, and selected lipid metabolism in 40 adolescent boys (11\u201316 y) with hepatic steatosis \u22655% (98% meeting the definition of MASLD). Participants were randomized to a low-free-sugar diet (LFSD) (n = 20) or usual diet (n = 20) for 8 weeks. Here, we employed untargeted\/targeted lipidomics to examine lipid adaptations associated with the LFSD and improvement of hepatic steatosis. Our LC-MS\/MS analysis revealed decreased triglycerides (TGs), diacylglycerols (DGs), cholesteryl esters (ChE), lysophosphatidylcholine (LPC), and phosphatidylcholine (PC) species with the diet intervention (p &lt; 0.05). Network analysis demonstrated significantly lower levels of palmitate-enriched TG species post-intervention, mirroring the previously shown reduction in DNL in response to the LFSD. Targeted oxylipins analysis revealed a decrease in the abundance of 8-isoprostane and 14,15-DiHET and an increase in 8,9-DiHET (p &lt; 0.05). Overall, we observed reductions in TGs, DGs, ChE, PC, and LPC species among participants in the LFSD group. These same lipids have been associated with MASLD progression; therefore, our findings may indicate normalization of key biological processes, including lipid metabolism, insulin resistance, and lipotoxicity. Additionally, our targeted oxylipins assay revealed novel changes in eicosanoids, suggesting improvements in oxidative stress. Future studies are needed to elucidate the mechanisms of these findings and prospects of these lipids as biomarkers of MASLD regression.<\/jats:p>","DOI":"10.3390\/metabo14040191","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T12:09:40Z","timestamp":1711627780000},"page":"191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Lipidome Changes Associated with a Diet-Induced Reduction in Hepatic Fat among Adolescent Boys with Metabolic Dysfunction-Associated Steatotic Liver Disease"],"prefix":"10.3390","volume":"14","author":[{"given":"Helaina E.","family":"Huneault","sequence":"first","affiliation":[{"name":"Nutrition & Health Sciences Doctoral Program, Laney Graduate School, Emory University, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory School of Medicine, Emory University, Atlanta, GA 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine C.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Section of Nutrition, Department of Pediatrics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory School of Medicine, Emory University, Atlanta, GA 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachery R.","family":"Jarrell","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5852-8065","authenticated-orcid":false,"given":"Zhulin","family":"He","sequence":"additional","affiliation":[{"name":"Pediatric Biostatistics Core, Department of Pediatrics, School of Medicine, Emory University, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla E.","family":"DeSantos","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University, Atlanta, GA 30322, USA"},{"name":"Children\u2019s Healthcare of Atlanta, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean A.","family":"Welsh","sequence":"additional","affiliation":[{"name":"Nutrition & Health Sciences Doctoral Program, Laney Graduate School, Emory University, Atlanta, GA 30322, USA"},{"name":"Children\u2019s Healthcare of Atlanta, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristal M.","family":"Maner-Smith","sequence":"additional","affiliation":[{"name":"Section of Nutrition, Department of Pediatrics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8855-3029","authenticated-orcid":false,"given":"Eric A.","family":"Ortlund","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory School of Medicine, Emory University, Atlanta, GA 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey B.","family":"Schwimmer","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Rady Children\u2019s Hospital San Diego, San Diego, CA 92123, USA"},{"name":"Department of Pediatrics, School of Medicine, University of California, San Diego, CA 92093, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam B.","family":"Vos","sequence":"additional","affiliation":[{"name":"Nutrition & Health Sciences Doctoral Program, Laney Graduate School, Emory University, Atlanta, GA 30322, USA"},{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University, Atlanta, GA 30322, USA"},{"name":"Children\u2019s Healthcare of Atlanta, Atlanta, GA 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101133","DOI":"10.1016\/j.aohep.2023.101133","article-title":"A multi-society Delphi consensus statement on new fatty liver disease nomenclature","volume":"29","author":"Rinella","year":"2023","journal-title":"Ann. 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