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Therefore, screening for early warning of this disease is important. This study used multi omics techniques to reveal the metabolic differences of dairy cows before RP onset and to find potential warning markers. Fecal samples and serum samples of 90 healthy Holstein cows were collected 7 days pre-calving; 10 healthy and 10 RP cows were enrolled according to normal expulsion of fetal membranes after calving. Fecal samples were subjected to 16S rRNA sequencing and untargeted metabolomics analysis, while plasma was analyzed using targeted metabolomics. Pathogenic bacteria levels increased in the intestines of cows with RP compared to those in healthy cows. Lipid metabolites constituted the largest proportion of differential metabolites between feces and plasma. Six potential warning markers for RP in cows were identified, including two fecal microbiomics markers (Oscillospiraceae UCG-005 and Escherichia-Shigella), one fecal untargeted metabolomics marker (N-acetylmuramic acid), and three plasma targeted metabolomics markers (glycylcholic acid-3 sulfate, 7-ketolithocholic acid, and 12-ketolithocholic acid). These biomarkers can predict RP occurrence in the early perinatal period. These results lay a theoretical foundation for early nutritional intervention and pathogenesis research in dairy cows.<\/jats:p>","DOI":"10.3390\/metabo14070386","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T14:15:49Z","timestamp":1721052949000},"page":"386","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Alteration of Fecal Microbiota, Fecal Metabolites, and Serum Metabolites in Dairy Cows with Pre-Retained Placenta"],"prefix":"10.3390","volume":"14","author":[{"given":"Tao","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Lanzhou Institute of Animal Husbandry and Veterinary Pharmaceutical, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenlong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Lanzhou Institute of Animal Husbandry and Veterinary Pharmaceutical, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengzhong","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry & Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404105, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueping","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5806","authenticated-orcid":false,"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8784-6003","authenticated-orcid":false,"given":"Shumin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuoting","family":"Yan","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Animal Husbandry and Veterinary Pharmaceutical, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0741-572X","authenticated-orcid":false,"given":"Suizhong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.cvfa.2014.07.009","article-title":"Transition cow nutrition and feeding management for disease prevention","volume":"30","author":"Sniffen","year":"2014","journal-title":"Vet. Clin. N. Am.-Food Anim. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.3168\/jds.2010-3758","article-title":"Effects of postpartum uterine diseases on milk production and culling in dairy cows","volume":"94","author":"Dubuc","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1016\/S0093-691X(00)00311-3","article-title":"Effect of disease on reproduction in the dairy cow: A meta-analysis","volume":"53","author":"Fourichon","year":"2000","journal-title":"Theriogenology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3168\/jds.S0022-0302(02)74107-6","article-title":"Decreased neutrophil function as a cause of retained placenta in dairy cattle","volume":"85","author":"Kimura","year":"2002","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4142\/jvs.2005.6.1.53","article-title":"Risk factors for retained placenta and the effect of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy cows","volume":"6","author":"Han","year":"2005","journal-title":"J. Vet. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.theriogenology.2020.10.007","article-title":"Farm and cow factors and their interactions on the incidence of retained placenta in holstein dairy cows","volume":"159","author":"Mahnani","year":"2021","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhang, G., Tobolski, D., Zwierzchowski, G., Mandal, R., Wishart, D.S., and Ametaj, B.N. (2021). A Targeted serum metabolomics GC-MS approach identifies predictive blood biomarkers for retained placenta in Holstein dairy cows. Metabolites, 11.","DOI":"10.3390\/metabo11090633"},{"key":"ref_8","first-page":"209","article-title":"Managing transition period health for reproductive performance in dairy cows","volume":"21","author":"Leblanc","year":"2014","journal-title":"Cattle Pract."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.2527\/1995.7392804x","article-title":"Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation","volume":"73","author":"Bell","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.theriogenology.2019.01.020","article-title":"The evaluation of superoxide dismutase activity, neutrophil function, and metabolic profile in cows with retained placenta","volume":"128","author":"Yazlik","year":"2019","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"106481","DOI":"10.1016\/j.anireprosci.2020.106481","article-title":"Blood urea nitrogen may serve as a predictive indicator of retained placenta in dairy cows","volume":"218","author":"Lu","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1017\/S1751731117002506","article-title":"Targeted metabolomics: New insights into pathobiology of retained placenta in dairy cows and potential risk biomarkers","volume":"12","author":"Dervishi","year":"2018","journal-title":"Animal"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.56808\/2985-1130.2734","article-title":"Comparative proteomic analysis of cow placentas with retained foetal membranes","volume":"46","author":"Fu","year":"2016","journal-title":"Thai J. Vet. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Du, Z., Luo, Z., Huang, Y., Zhou, T., Ma, L., Wu, D., Yao, X., Shen, L., Yu, S., and Yong, K. (2024). Screening for potential warning biomarkers in cows with ketosis based on host-microbiota co-metabolism analysis. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1373402"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Luo, Z., Yong, K., Luo, Q., Du, Z., Ma, L., Huang, Y., Zhou, T., Yao, X., Shen, L., and Yu, S. (2022). Altered fecal microbiome and correlations of the metabolome with plasma metabolites in dairy cows with left displaced abomasum. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.01972-22"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7668","DOI":"10.3168\/jds.2021-21654","article-title":"Discrepancies among healthy, subclinical mastitic, and clinical mastitic cows in fecal microbiome and metabolome and serum metabolome","volume":"105","author":"Wang","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1039\/C1AN15605E","article-title":"Modern analytical techniques in metabolomics analysis","volume":"137","author":"Zhang","year":"2012","journal-title":"Analyst"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1576\/toag.13.3.189.27672","article-title":"\u2018Omic\u2019 technologies: Genomics, transcriptomics, proteomics and metabolomics","volume":"13","author":"Horgan","year":"2011","journal-title":"Obstet. Gynaecol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Luo, Z., Yong, K., Du, Z., Huang, Y., Zhou, T., Ma, L., Yao, X., Shen, L., Yu, S., and Yan, Z. (2023). Association between tryptophan metabolism and inflammatory biomarkers in dairy cows with ketosis. Metabolites, 13.","DOI":"10.3390\/metabo13030333"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1128\/CMR.00022-13","article-title":"Recent advances in understanding enteric pathogenic Escherichia coli","volume":"26","author":"Croxen","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pang, M., Xie, X., Bao, H., Sun, L., He, T., Zhao, H., Zhou, Y., Zhang, L., Zhang, H., and Wei, R. (2018). Insights into the Bovine Milk Microbiota in Dairy Farms with Different Incidence Rates of Subclinical Mastitis. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02379"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.3945\/ajcn.113.058743","article-title":"Gut microbiota after gastric bypass in human obesity: Increased richness and associations of bacterial genera with adipose tissue genes","volume":"98","author":"Kong","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5967","DOI":"10.3168\/jds.2015-10727","article-title":"Invited review: Role of bacterial endotoxins in the etiopathogenesis of periparturient diseases of transition dairy cows","volume":"99","author":"Eckel","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, H., Ma, L., Li, Z., Yin, J., Tan, B., Chen, J., Jiang, Q., and Ma, X. (2021). Evolution of the gut microbiota and its fermentation characteristics of Ningxiang pigs at the young stage. Animals, 11.","DOI":"10.3390\/ani11030638"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/nrmicro3344","article-title":"The gut microbiota, bacterial metabolites and colorectal cancer","volume":"12","author":"Louis","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_26","first-page":"2789","article-title":"Acetic acid alleviates the inflammatory response and liver injury in septic mice by increasing the expression of TRIM40","volume":"17","author":"Yang","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tian, X., Hellman, J., Horswill, A.R., Crosby, H.A., Francis, K.P., and Prakash, A. (2019). Elevated gut microbiome-derived propionate levels are associated with reduced sterile lung inflammation and bacterial immunity in mice. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00518"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhai, S., Qin, S., Li, L., Zhu, L., Zou, Z., and Wang, L. (2019). Dietary butyrate suppresses inflammation through modulating gut microbiota in high-fat diet-fed mice. Fems Microbiol. Lett., 366.","DOI":"10.1093\/femsle\/fnz153"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1054\/clnu.2001.0409","article-title":"Gut barrier function and bacterial translocation in humans","volume":"20","author":"Gabe","year":"2001","journal-title":"Clin. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/s41588-018-0135-7","article-title":"The fecal metabolome as a functional readout of the gut microbiome","volume":"50","author":"Zierer","year":"2018","journal-title":"Nat. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0006-291X(71)90543-2","article-title":"Occurrence of non-N-substituted glucosamine residues in lysozyme-resistant peptidoglycan from Bacillus cereus cell walls","volume":"42","author":"Araki","year":"1971","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1126\/science.aaf3552","article-title":"A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens","volume":"353","author":"Rangan","year":"2016","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1038\/ni1207-1269","article-title":"Bacterial cell walls, innate immunity and immunoadjuvants","volume":"8","author":"Strominger","year":"2007","journal-title":"Nat. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1017\/S0007114521005031","article-title":"Riboflavin is an antioxidant: A review update","volume":"128","author":"Olfat","year":"2022","journal-title":"Br. J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.3945\/jn.111.142257","article-title":"Foods, fortificants, and supplements: Where do Americans get their nutrients?","volume":"141","author":"Fulgoni","year":"2011","journal-title":"J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1111\/j.1471-4159.2006.03913.x","article-title":"The glutamate\/GABA-glutamine cycle: Aspects of transport, neurotransmitter homeostasis and ammonia transfer","volume":"98","author":"Bak","year":"2006","journal-title":"J. Neurochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/s11892-011-0187-x","article-title":"Bile acid metabolism and the pathogenesis of type 2 diabetes","volume":"11","author":"Prawitt","year":"2011","journal-title":"Curr. Diabetes Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1515\/jtim-2016-0018","article-title":"Obesity diabetes and the role of bile acids in metabolism","volume":"4","author":"Tomkin","year":"2016","journal-title":"J. Transl. Intern. Med."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mccabe, M., Waters, S., Morris, D., Kenny, D., Lynn, D., and Creevey, C. (2012). RNA-seq analysis of differential gene expression in liver from lactating dairy cows divergent in negative energy balance. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-193"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1194\/jlr.R900007-JLR200","article-title":"Bile acids as regulatory molecules","volume":"50","author":"Hylemon","year":"2009","journal-title":"J. Lipid Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s13238-020-00804-9","article-title":"Targeting the alternative bile acid synthetic pathway for metabolic diseases","volume":"12","author":"Jia","year":"2021","journal-title":"Protein Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.chom.2020.01.021","article-title":"Dysbiosis-induced secondary bile acid deficiency promotes intestinal inflammation","volume":"27","author":"Sinha","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1159\/000450913","article-title":"Bile acids and deregulated cholangiocyte autophagy in primary biliary cholangitis","volume":"35","author":"Sasaki","year":"2017","journal-title":"Dig. Dis."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/14\/7\/386\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:16:58Z","timestamp":1760109418000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/14\/7\/386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,15]]},"references-count":43,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["metabo14070386"],"URL":"https:\/\/doi.org\/10.3390\/metabo14070386","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,15]]}}}