{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:59:00Z","timestamp":1789185540098,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360905"],"award-info":[{"award-number":["32360905"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LGLZ202306"],"award-info":[{"award-number":["LGLZ202306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21KJCX0011"],"award-info":[{"award-number":["21KJCX0011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High School Projects of Xichang University","award":["32360905"],"award-info":[{"award-number":["32360905"]}]},{"name":"High School Projects of Xichang University","award":["LGLZ202306"],"award-info":[{"award-number":["LGLZ202306"]}]},{"name":"High School Projects of Xichang University","award":["21KJCX0011"],"award-info":[{"award-number":["21KJCX0011"]}]},{"name":"Key Research and development Project of Liangshan Science and Technology Bureau","award":["32360905"],"award-info":[{"award-number":["32360905"]}]},{"name":"Key Research and development Project of Liangshan Science and Technology Bureau","award":["LGLZ202306"],"award-info":[{"award-number":["LGLZ202306"]}]},{"name":"Key Research and development Project of Liangshan Science and Technology Bureau","award":["21KJCX0011"],"award-info":[{"award-number":["21KJCX0011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Bile, a crucial fluid produced continuously by the liver, plays an essential role in digestion within the small intestine. Beyond its primary function in lipid digestion, bile also acts as a pathway for the elimination of various endogenous and exogenous substances. There have been limited studies focusing on interspecies differences. This study offers a comprehensive analysis of bile acid (BA) composition and its correlation with gene expression patterns across six different species, including mammals and poultry, through combining Liquid Chromatography-Mass Spectrometry (LC-MS) and transcriptome sequencing. The BA profiles revealed distinct metabolite clusters: D-glucuronic acid (GLCA) and glycochenodeoxycholic acid (GCDCA) were predominant in mammals, while taurolithocholic acid (TLCA) and T-alpha-MCA were prevalent in poultry, highlighting species-specific BA compositions. Differentially abundant metabolites, particularly GDCA, glycohyodeoxycholic acid (GHDCA) and taurodeoxycholic acid (TDCA) showed significant variations across species, with pigs showing the highest BA content. Transcriptome analysis of the liver and small intestine tissues of 56 cDNA libraries across the six species revealed distinct mRNA expression patterns. These patterns clustered samples into broad categories based on tissue type and phylogenetic relationships. Furthermore, the correlation between gene expression and BA content was examined, identifying the top 20 genes with significant associations. These genes potentially serve as biomarkers for BA regulation.<\/jats:p>","DOI":"10.3390\/metabo14080451","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T04:29:57Z","timestamp":1723782597000},"page":"451","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bile Acid Composition and Transcriptome Analysis of the Liver and Small Intestine in Different Species"],"prefix":"10.3390","volume":"14","author":[{"given":"Dongming","family":"Qi","sequence":"first","affiliation":[{"name":"College of Animal Science, Xichang University, Xichang 615000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maosen","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Shanxi Agricultural University, Taiyuan 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningnan County Bureau of Agriculture and Rural Affairs, Xichang 615400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binlong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science, Xichang University, Xichang 615000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116307","DOI":"10.1016\/j.trac.2021.116307","article-title":"Current approaches to the analysis of bile and the determination of bile acids in various biological matrices as supportive tools to traditional diagnostic testing for liver dysfunction and biliary diseases","volume":"142","author":"Bojko","year":"2021","journal-title":"TrAC Trends Anal. 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