{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:22:40Z","timestamp":1760145760023,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T00:00:00Z","timestamp":1723852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"GreenWin","award":["convention 7276"],"award-info":[{"award-number":["convention 7276"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The decontamination of polluted soils is a major socioeconomic issue in many industrialized countries. In situ remediation approaches are nowadays preferred to ex situ techniques, but they require among others the use of bioindicators, which are sensitive to the progressive depollution on health effects. Animal species have been mainly used so far to monitor aquatic and air pollution. Current research focuses on the development of living indicators of soil pollution. In this study, the garden snail Helix aspersa maxima was acutely exposed to cadmium, one major soil contaminant causing severe health effects, including nephrotoxicity. Kidney and hemolymph were sampled and analyzed by a 1H-NMR-based metabonomic approach. Shortly after Cd exposure, numerous metabolic changes occurred in the hemolymph and kidney extracts. Altogether, they were indicative of a switch in energy sources from the Krebs cycle towards b-oxidation and the utilization of stored galactogen polysaccharides. Then, the activation of antioxidant defenses in the renal cells was suggested by the alteration in some precursors of glutathione synthesis, such as glutamate, and by the release of the antioxidant anserin. Cell membrane damage was evidenced by the increased levels of some osmolytes, betaine and putrescine, as well as by a membrane repair mechanism involving choline. Finally, the development of metabolic acidosis was suggested by the elevation in 3-HMG in the hemolymph, and the more pronounced lysine levels were consistent with acute excretion troubles. Cd-induced renal damage was objectified by the increased level of riboflavin, a recognized biomarker of nephrotoxicity.<\/jats:p>","DOI":"10.3390\/metabo14080455","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T06:41:31Z","timestamp":1724049691000},"page":"455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolomic Prediction of Cadmium Nephrotoxicity in the Snail Helix aspersa maxima"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4633-2812","authenticated-orcid":false,"given":"Aude","family":"Devalckeneer","sequence":"first","affiliation":[{"name":"Laboratory of Human Biology & Toxicology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Bouviez","sequence":"additional","affiliation":[{"name":"Laboratory of Human Biology & Toxicology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandine","family":"Gautier","sequence":"additional","affiliation":[{"name":"HEPH (Haute Ecole Provinciale du Hainaut), Condorcet, 7000 Mons, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-2795","authenticated-orcid":false,"given":"Jean-Marie","family":"Colet","sequence":"additional","affiliation":[{"name":"Laboratory of Human Biology & Toxicology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,17]]},"reference":[{"key":"ref_1","unstructured":"Ribera, D., and Taberly, J. 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