{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:09:31Z","timestamp":1790698171167,"version":"4.1.0"},"reference-count":84,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Chongqing, China","award":["CSTB2022NSCQ-MSX1602"],"award-info":[{"award-number":["CSTB2022NSCQ-MSX1602"]}]},{"name":"Natural Science Foundation of Chongqing, China","award":["GZC20231869"],"award-info":[{"award-number":["GZC20231869"]}]},{"name":"Natural Science Foundation of Chongqing, China","award":["2023NSFSC0234"],"award-info":[{"award-number":["2023NSFSC0234"]}]},{"name":"Natural Science Foundation of Chongqing, China","award":["KJZD-M202303501"],"award-info":[{"award-number":["KJZD-M202303501"]}]},{"name":"Postdoctoral Fellowship Program of CPSF under Grant","award":["CSTB2022NSCQ-MSX1602"],"award-info":[{"award-number":["CSTB2022NSCQ-MSX1602"]}]},{"name":"Postdoctoral Fellowship Program of CPSF under Grant","award":["GZC20231869"],"award-info":[{"award-number":["GZC20231869"]}]},{"name":"Postdoctoral Fellowship Program of CPSF under Grant","award":["2023NSFSC0234"],"award-info":[{"award-number":["2023NSFSC0234"]}]},{"name":"Postdoctoral Fellowship Program of CPSF under Grant","award":["KJZD-M202303501"],"award-info":[{"award-number":["KJZD-M202303501"]}]},{"name":"Natural Science Foundation of Sichuan, China","award":["CSTB2022NSCQ-MSX1602"],"award-info":[{"award-number":["CSTB2022NSCQ-MSX1602"]}]},{"name":"Natural Science Foundation of Sichuan, China","award":["GZC20231869"],"award-info":[{"award-number":["GZC20231869"]}]},{"name":"Natural Science Foundation of Sichuan, China","award":["2023NSFSC0234"],"award-info":[{"award-number":["2023NSFSC0234"]}]},{"name":"Natural Science Foundation of Sichuan, China","award":["KJZD-M202303501"],"award-info":[{"award-number":["KJZD-M202303501"]}]},{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["CSTB2022NSCQ-MSX1602"],"award-info":[{"award-number":["CSTB2022NSCQ-MSX1602"]}]},{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["GZC20231869"],"award-info":[{"award-number":["GZC20231869"]}]},{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["2023NSFSC0234"],"award-info":[{"award-number":["2023NSFSC0234"]}]},{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["KJZD-M202303501"],"award-info":[{"award-number":["KJZD-M202303501"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows. Methods: Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing. Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations \u2265 1 mmol\/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of Bacillus, Solibacillus, Dorea, and Romboutsia were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT. Conclusions: DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites.<\/jats:p>","DOI":"10.3390\/metabo15010058","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T06:46:10Z","timestamp":1737009970000},"page":"58","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7573-2021","authenticated-orcid":false,"given":"Zheng","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Department of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing  404105, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing  404105, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8841-3931","authenticated-orcid":false,"given":"Zhengzhong","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Department of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing  404105, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenlong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueping","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5806","authenticated-orcid":false,"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8784-6003","authenticated-orcid":false,"given":"Shumin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0741-572X","authenticated-orcid":false,"given":"Suizhong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"ADSA Foundation Scholar Award. 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