{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T21:39:41Z","timestamp":1767994781275,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T00:00:00Z","timestamp":1737331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82204979"],"award-info":[{"award-number":["82204979"]}]},{"name":"National Natural Science Foundation of China","award":["81973756"],"award-info":[{"award-number":["81973756"]}]},{"name":"National Natural Science Foundation of China","award":["No.2023ZR078"],"award-info":[{"award-number":["No.2023ZR078"]}]},{"name":"National Natural Science Foundation of China","award":["No.Y202454996"],"award-info":[{"award-number":["No.Y202454996"]}]},{"name":"National Natural Science Foundation of China","award":["No.2021RCZXZK19"],"award-info":[{"award-number":["No.2021RCZXZK19"]}]},{"name":"Zhejiang Province Traditional Chinese medicine health science and technology project","award":["82204979"],"award-info":[{"award-number":["82204979"]}]},{"name":"Zhejiang Province Traditional Chinese medicine health science and technology project","award":["81973756"],"award-info":[{"award-number":["81973756"]}]},{"name":"Zhejiang Province Traditional Chinese medicine health science and technology project","award":["No.2023ZR078"],"award-info":[{"award-number":["No.2023ZR078"]}]},{"name":"Zhejiang Province Traditional Chinese medicine health science and technology project","award":["No.Y202454996"],"award-info":[{"award-number":["No.Y202454996"]}]},{"name":"Zhejiang Province Traditional Chinese medicine health science and technology project","award":["No.2021RCZXZK19"],"award-info":[{"award-number":["No.2021RCZXZK19"]}]},{"name":"General research project of the Zhejiang provincial department of education","award":["82204979"],"award-info":[{"award-number":["82204979"]}]},{"name":"General research project of the Zhejiang provincial department of education","award":["81973756"],"award-info":[{"award-number":["81973756"]}]},{"name":"General research project of the Zhejiang provincial department of education","award":["No.2023ZR078"],"award-info":[{"award-number":["No.2023ZR078"]}]},{"name":"General research project of the Zhejiang provincial department of education","award":["No.Y202454996"],"award-info":[{"award-number":["No.Y202454996"]}]},{"name":"General research project of the Zhejiang provincial department of education","award":["No.2021RCZXZK19"],"award-info":[{"award-number":["No.2021RCZXZK19"]}]},{"name":"School-level scientific research project (talent special) of Zhejiang Chinese Medical University","award":["82204979"],"award-info":[{"award-number":["82204979"]}]},{"name":"School-level scientific research project (talent special) of Zhejiang Chinese Medical University","award":["81973756"],"award-info":[{"award-number":["81973756"]}]},{"name":"School-level scientific research project (talent special) of Zhejiang Chinese Medical University","award":["No.2023ZR078"],"award-info":[{"award-number":["No.2023ZR078"]}]},{"name":"School-level scientific research project (talent special) of Zhejiang Chinese Medical University","award":["No.Y202454996"],"award-info":[{"award-number":["No.Y202454996"]}]},{"name":"School-level scientific research project (talent special) of Zhejiang Chinese Medical University","award":["No.2021RCZXZK19"],"award-info":[{"award-number":["No.2021RCZXZK19"]}]},{"name":"Public Platform of Medical Research Center, Academy of Chinese Medical Science, Zhejiang Chinese Medical University","award":["82204979"],"award-info":[{"award-number":["82204979"]}]},{"name":"Public Platform of Medical Research Center, Academy of Chinese Medical Science, Zhejiang Chinese Medical University","award":["81973756"],"award-info":[{"award-number":["81973756"]}]},{"name":"Public Platform of Medical Research Center, Academy of Chinese Medical Science, Zhejiang Chinese Medical University","award":["No.2023ZR078"],"award-info":[{"award-number":["No.2023ZR078"]}]},{"name":"Public Platform of Medical Research Center, Academy of Chinese Medical Science, Zhejiang Chinese Medical University","award":["No.Y202454996"],"award-info":[{"award-number":["No.Y202454996"]}]},{"name":"Public Platform of Medical Research Center, Academy of Chinese Medical Science, Zhejiang Chinese Medical University","award":["No.2021RCZXZK19"],"award-info":[{"award-number":["No.2021RCZXZK19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Postoperative ileus (POI) is a common postoperative clinical complication that significantly affects postoperative rehabilitation and quality of life in patients and can even produce secondary complications, leading to serious consequences. External treatment using Shenhuang Plaster (SHP) (Shenque acupoint administration) has definite effects and unique advantages in the prevention and treatment of POI, but its mechanism is not completely clear. In this study, we investigated the therapeutic mechanism behind the effect of Shenhuang Plaster applied to the Shenque acupoint on gastrointestinal motility in POI mice based on metabolomics. Materials and Methods: C57BL\/6 mice were divided into three groups: blank control (Ctrl), model (POI), and intervention (POI + SHP) groups. SHP treatment was started 3 days before modeling. We employed several behavioral tests and gastrointestinal transit function measurements and performed qRT-PCR analysis, 16S rRNA gene sequencing, and metabolomics analysis on serum metabolites. Results: We found that SHP could reduce the mRNA expression of inflammatory mediators in the smooth muscle tissue of the small intestine, regulate the structure and function of the intestinal microbiota, and modulate serum phenylalanine, carnitine, and glutamic acid levels. Conclusions: POI mice had obvious intestinal flora disorders and metabolic disorders of amino acids and their derivatives, and there was a significant correlation between differential flora and differential metabolites. SHP could effectively regulate the concentration of intestinal flora and serum metabolites and the metabolic pathway related to amino acids in vivo and, ultimately, achieve a therapeutic purpose in POI. In this study, it was found, for the first time, that applying SHP to the Shenque acupoint could effectively regulate the serum metabolites of phenylalanine, carnitine, and glutamate, and improve postoperative intestinal motile disturbance through association with the intestinal flora.<\/jats:p>","DOI":"10.3390\/metabo15010065","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T12:32:52Z","timestamp":1737376372000},"page":"65","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolics-Based Study on the Therapeutic Mechanism Behind the Effect of Shenhuang Plaster Applied to the Shenque Acupoint on Gastrointestinal Motility in POI Mice"],"prefix":"10.3390","volume":"15","author":[{"given":"Yanan","family":"Shi","sequence":"first","affiliation":[{"name":"The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglei","family":"Wu","sequence":"additional","affiliation":[{"name":"The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongyun","family":"Wang","sequence":"additional","affiliation":[{"name":"The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7916-3408","authenticated-orcid":false,"given":"Qiuhua","family":"Sun","sequence":"additional","affiliation":[{"name":"The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.3349\/ymj.2013.54.6.1336","article-title":"Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig","volume":"54","author":"Choi","year":"2013","journal-title":"Yonsei Med. 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