{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:39:01Z","timestamp":1792035541380,"version":"4.4.0"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BudDiag","award":["CZ.02.01.01\/00\/23_021\/0008906"],"award-info":[{"award-number":["CZ.02.01.01\/00\/23_021\/0008906"]}]},{"name":"European Union","award":["CZ.02.01.01\/00\/23_021\/0008906"],"award-info":[{"award-number":["CZ.02.01.01\/00\/23_021\/0008906"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Fatty acids (FAs) represent a ubiquitous class of nonpolar alkyl carboxylate metabolites with diverse biological functions. Nutrition, metabolism, and endogenous and exogenous stress influence the overall FA metabolic status and transport via the bloodstream. FAs esterified in lipids are of particular interest, as they represent promising biomarkers of pathological diseases and nutritional status. Methods: Here, we report a validated gas chromatographic-mass spectrometric (GC-MS) method for the quantitative analysis of 32 FAs exclusively bound in esterified lipids. The developed sample preparation protocol comprises three steps using only 5 \u00b5L of human serum for Folch extraction, sodium methoxide-catalyzed transesterification in tert-butyl methyl ether, and re-extraction in isooctane prior to a quantitative GC-MS analysis with positive ion chemical ionization (PICI) and selected ion monitoring (SIM). Results: The base-catalyzed transmethylation step was studied for 14 lipid classes and was found to be efficient under mild conditions for all major esterified lipids but not for free FAs, lipid amides, or sphingolipids. To minimize matrix effects and instrument bias, internal fatty acid trideuteromethyl esters (D3-FAME) standards were prepared through isotope-coded derivatization with D3-labeled methylchloroformate\/methanol medium mixed with each transmethylated serum extract for the assay. The method was validated according to FDA guidelines and evaluated by analyzing NIST SRM 2378 Serum 1 and sera from three healthy donors. Conclusions: The measured quantitative FA values are consistent with the reference data of SRM 2378, and they demonstrate the application potential of the described method for general FA analysis in esterified lipids as a novel complementary tool for lipidomics, as well as for the analysis of membrane FAs in dry blood spots and red blood cells.<\/jats:p>","DOI":"10.3390\/metabo15020104","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:00:28Z","timestamp":1738900828000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A New, Validated GC-PICI-MS Method for the Quantification of 32 Lipid Fatty Acids via Base-Catalyzed Transmethylation and the Isotope-Coded Derivatization of Internal Standards"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9875-668X","authenticated-orcid":false,"given":"Petr","family":"Vodr\u00e1\u017eka","sequence":"first","affiliation":[{"name":"Biology Centre, Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Department of Chemistry of Natural Compounds, University of Chemistry and Technology, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3270-1763","authenticated-orcid":false,"given":"Lucie","family":"\u0158imn\u00e1\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Biology Centre, Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5390-2445","authenticated-orcid":false,"given":"Petra","family":"Berkov\u00e1","sequence":"additional","affiliation":[{"name":"Biology Centre, Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3152-5861","authenticated-orcid":false,"given":"Jan","family":"Vojt\u00ed\u0161ek","sequence":"additional","affiliation":[{"name":"Hospital \u010cesk\u00e9 Bud\u011bjovice, B. N\u011bmcov\u00e9 585\/54, 370 01 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8339-985X","authenticated-orcid":false,"given":"Miroslav","family":"Verner","sequence":"additional","affiliation":[{"name":"Hospital \u010cesk\u00e9 Bud\u011bjovice, B. N\u011bmcov\u00e9 585\/54, 370 01 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-3132","authenticated-orcid":false,"given":"Martin","family":"Moos","sequence":"additional","affiliation":[{"name":"Biology Centre, Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2754-6372","authenticated-orcid":false,"given":"Petr","family":"\u0160imek","sequence":"additional","affiliation":[{"name":"Biology Centre, Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Djuricic, I., and Calder, P.C. (2021). 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