{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:59:26Z","timestamp":1760057966207,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dalian Science and Technology Talent Innovation Support Plan","award":["2022RQ038","2022QNRC001"],"award-info":[{"award-number":["2022RQ038","2022QNRC001"]}]},{"name":"Young Elite Scientists Sponsorship Program by CAST","award":["2022RQ038","2022QNRC001"],"award-info":[{"award-number":["2022RQ038","2022QNRC001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Osteoporosis is frequently observed in patients with chronic pancreatitis, and both conditions are closely associated with systemic metabolic disorders. However, the underlying mechanisms linking chronic pancreatitis and osteoporosis remain unclear. Methods: In this study, we utilized high\u2212performance liquid chromatography\u2013mass spectrometry (HPLC\u2212MS) to conduct metabolomics and lipidomics analyses on pancreatic, serum, and other tissues from a mouse model of chronic pancreatitis\u2212induced osteoporosis (CP\u2212OP), with the aim to elucidate the metabolism\u2212related pathogenic mechanisms of CP\u2212OP. Results: We identified over 405 metabolites and 445 lipids, and our findings revealed that several metabolites involving the tricarboxylic acid (TCA) cycle, as well as triacylglycerols and diacylglycerols with higher saturation, were significantly increased in the CP\u2212OP model. In contrast, triglycerides with higher unsaturation were decreased. Differential pathways were enriched in n\u22123 long\u2212chain polyunsaturated fatty acid metabolism in both pancreatic and bone tissues, and these pathways exhibited positive correlations with bone\u2212related parameters. Furthermore, the modulation of these polyunsaturated fatty acids by Qingyi granules demonstrated significant therapeutic effects on CP\u2212OP, as validated in mouse models. Conclusions: Through the metabolomics approach, we uncovered that disorders in polyunsaturated fatty acids play a critical role in the pathogenesis of CP\u2212OP. This study not only enhances our understanding of the pathogenesis of CP\u2212OP but also highlights the therapeutic potential of targeting polyunsaturated fatty acids as a future intervention strategy for osteoporosis treatment.<\/jats:p>","DOI":"10.3390\/metabo15030173","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T10:15:03Z","timestamp":1740996903000},"page":"173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomics Approach Revealed Polyunsaturated Fatty Acid Disorders as Pathogenesis for Chronic Pancreatitis\u2212Induced Osteoporosis in Mice"],"prefix":"10.3390","volume":"15","author":[{"given":"Xinlin","family":"Liu","sequence":"first","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Institute (College) of Integrative Medicine, Dalian Medical University, Dalian 116044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenglin","family":"Hu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunshu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Institute (College) of Integrative Medicine, Dalian Medical University, Dalian 116044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shurong","family":"Ma","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Guantao County People\u2019s Hospital, Handan 057750, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Shang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3639-3363","authenticated-orcid":false,"given":"Peiyuan","family":"Yin","sequence":"additional","affiliation":[{"name":"Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"},{"name":"Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1097\/MPA.0000000000000715","article-title":"Peering into the \u201cBlack Box\u201d of the Complex Chronic Pancreatitis Syndrome","volume":"45","author":"Whitcomb","year":"2016","journal-title":"Pancreas"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.pan.2016.02.001","article-title":"Chronic Pancreatitis: An international draft consensus proposal for a new mechanistic definition","volume":"16","author":"Whitcomb","year":"2016","journal-title":"Pancreatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4008","DOI":"10.1007\/s10620-020-06732-2","article-title":"Prevalence and Risk Factors for Osteopathy in Chronic Pancreatitis","volume":"66","author":"Tang","year":"2021","journal-title":"Dig. 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