{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:47:02Z","timestamp":1765234022868,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Health","award":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"],"award-info":[{"award-number":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"]}]},{"DOI":"10.13039\/100000199","name":"United States Department of Agriculture","doi-asserted-by":"publisher","award":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"],"award-info":[{"award-number":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"],"award-info":[{"award-number":["SC3 GM122630","R25 GM131956","2022-77040-37645","1919908"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: There is a high demand for long-term postharvest storage of valuable perishables with high-quality preservation and minimal product loss due to decay and physiological disorders. Postharvest low-pressure storage (LPS) provides a viable option for many fruits. While recent studies have presented the details of technology, this pilot study presents the metabolomics changes due to the hypobaric storage of pomegranates as a model system. Methods: Nuclear magnetic resonance (NMR)-based metabolomics studies were performed on pomegranate fruit tissues, comparing fruit stored under LPS conditions versus the traditional storage system, with modified atmosphere packaging (MAP) as the control. The metabolomic changes in the exocarp, mesocarp, and arils were measured using 1H NMR spectroscopy, and the results were analyzed using multivariate statistics. Results: Distinguishable differences were noted between the MAP and LPS conditions in fruit quality attributes and metabolite profiles. Sucrose levels in the aril, mesocarp, and exocarp samples were higher under LPS, while sucrose levels were reduced in MAP. In addition, alanine levels were more abundant in the mesocarp and exocarp samples, and ethanol concentration decreased in the exocarp samples, albeit less significantly. Conclusions: This pilot investigation shows the potential for using NMR as a valuable assessment tool for monitoring the performance of viable long-term storage conditions in horticultural commodities.<\/jats:p>","DOI":"10.3390\/metabo15080507","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:55:37Z","timestamp":1753872937000},"page":"507","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Postharvest NMR Metabolomic Profiling of Pomegranates Stored Under Low-Pressure Conditions: A Pilot Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Keeton H.","family":"Montgomery","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University, Fresno, CA 93740, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8180-6545","authenticated-orcid":false,"given":"Aya","family":"Elhabashy","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University, Fresno, CA 93740, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendon M.","family":"Anthony","sequence":"additional","affiliation":[{"name":"Department of Fruit Sciences, RipeLocker, Inc., Bainbridge Island, WA 98110, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Ki","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Fruit Sciences, RipeLocker, Inc., Bainbridge Island, WA 98110, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0726-950X","authenticated-orcid":false,"given":"Viswanathan V.","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University, Fresno, CA 93740, USA"},{"name":"Department of Pathology and Molecular Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2212\/spr.2015.4.3","article-title":"Alternative technologies to control postharvest diseases of pomegranate","volume":"11","author":"Teksur","year":"2015","journal-title":"Stewart Postharvest Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yam, K.L., and Lee, D.S. 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