{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:36:54Z","timestamp":1792708614036,"version":"4.5.0"},"reference-count":39,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Acute myeloid leukemia (AML), the most common acute leukemia among adults, poses significant therapeutic challenges due to diagnostic limitations and the frequent development of treatment resistance. While genomics-based approaches have advanced, DNA aberrations do not always reflect the expression levels of genes and proteins, which are more tightly connected to disease phenotypes. Recently, the role of the gut microbiota in AML has gained increasing attention. AML patients often exhibit gut microbiota dysbiosis, which is linked to disease progression and heightened infection risk. Mounting evidence indicates that gut microbiota metabolism influences hematopoiesis and immune function via the \u201cgut-bone marrow axis,\u201d with microbiota composition and diversity significantly affecting treatment outcomes and prognosis. High-throughput sequencing and metabolomics have identified correlations between gut microbiota composition and its metabolic products with AML clinical characteristics, paving the way for new biomarkers in diagnosis and prognosis. Additionally, treatments such as fecal microbiota transplantation (FMT) show promise in enhancing chemotherapy efficacy and improving patient outcomes. This review highlights recent advances in understanding the role of the gut microbiota in AML and explores new perspectives for its diagnosis and treatment.<\/jats:p>","DOI":"10.3390\/metabo15090568","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T06:08:37Z","timestamp":1756102117000},"page":"568","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Gut Microbiota in Acute Myeloid Leukemia: From Biomarkers to Interventions"],"prefix":"10.3390","volume":"15","author":[{"given":"Meifen","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Nursing, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China"},{"name":"Lishui People\u2019s Hospital, Lishui 321400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixia","family":"Ji","sequence":"additional","affiliation":[{"name":"Lishui People\u2019s Hospital, Lishui 321400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yebo","family":"Zhong","sequence":"additional","affiliation":[{"name":"Lishui People\u2019s Hospital, Lishui 321400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewen","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Nursing, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"ref_1","first-page":"328","article-title":"Acute Myeloid Leukemia-Genetic Alterations and Their Clinical Prognosis","volume":"11","year":"2017","journal-title":"Int. J. Hematol. Oncol. Stem Cell Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1002\/cncr.33458","article-title":"De novo acute myeloid leukemia: A population-based study of outcome in the United States based on the Surveillance, Epidemiology, and End Results (SEER) database, 1980 to 2017","volume":"127","author":"Sasaki","year":"2021","journal-title":"Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1182\/bloodadvances.2019001267","article-title":"Outcomes of older patients with NPM1-mutated AML: Current treatments and the promise of venetoclax-based regimens","volume":"4","author":"Lachowiez","year":"2020","journal-title":"Blood Adv."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s13045-021-01110-5","article-title":"A precision medicine classification for treatment of acute myeloid leukemia in older patients","volume":"14","author":"Mims","year":"2021","journal-title":"J. Hematol. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2141.1976.tb03563.x","article-title":"Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group","volume":"33","author":"Bennett","year":"1976","journal-title":"Br. J. Haematol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1038\/s41375-022-01613-1","article-title":"The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Myeloid and Histiocytic\/Dendritic Neoplasms","volume":"36","author":"Khoury","year":"2022","journal-title":"Leukemia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1182\/blood.2022016867","article-title":"Diagnosis and management of AML in adults: 2022 recommendations from an international expert panel on behalf of the ELN","volume":"140","author":"Wei","year":"2022","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/clinchem\/hvad171","article-title":"Acute Myeloid Leukemia Genomics: Impact on Care and Remaining Challenges","volume":"70","author":"Eisfeld","year":"2024","journal-title":"Clin. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Awada, H., Mustafa Ali, M.K., Thapa, B., Awada, H., Seymour, L., Liu, L., Gurnari, C., Kishtagari, A., Wang, E., and Baer, M.R. (2022). A Focus on Intermediate-Risk Acute Myeloid Leukemia: Sub-Classification Updates and Therapeutic Challenges. Cancers, 14.","DOI":"10.3390\/cancers14174166"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1182\/blood.2020006158","article-title":"TP53 mutations in myelodysplastic syndromes and secondary AML confer an immunosuppressive phenotype","volume":"136","author":"Sallman","year":"2020","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.2147\/OTT.S191621","article-title":"Mechanisms of drug resistance in acute myeloid leukemia","volume":"12","author":"Zhang","year":"2019","journal-title":"Onco Targets Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e1373208","DOI":"10.1080\/21688370.2017.1373208","article-title":"Intestinal barrier and gut microbiota: Shaping our immune responses throughout life","volume":"5","author":"Takiishi","year":"2017","journal-title":"Tissue Barriers"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41579-020-0433-9","article-title":"Gut microbiota in human metabolic health and disease","volume":"19","author":"Fan","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1038\/s42255-022-00649-y","article-title":"Gut Parabacteroides merdae protects against cardiovascular damage by enhancing branched-chain amino acid catabolism","volume":"4","author":"Qiao","year":"2022","journal-title":"Nat. Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1038\/s41467-022-30240-8","article-title":"Gut microbiota regulates acute myeloid leukaemia via alteration of intestinal barrier function mediated by butyrate","volume":"13","author":"Wang","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1016\/j.clnu.2023.09.021","article-title":"Gut microbiota alterations induced by intensive chemotherapy in acute myeloid leukaemia patients are associated with gut barrier dysfunction and body weight loss","volume":"42","author":"Lecop","year":"2023","journal-title":"Clin. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liu, J., Wei, Y., Jia, W., Can, C., Wang, R., Yang, X., Gu, C., Liu, F., Ji, C., and Ma, D. (2022). Chenodeoxycholic acid suppresses AML progression through promoting lipid peroxidation via ROS\/p38 MAPK\/DGAT1 pathway and inhibiting M2 macrophage polarization. Redox Biol., 56.","DOI":"10.1016\/j.redox.2022.102452"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.freeradbiomed.2024.01.005","article-title":"Propionate promotes ferroptosis and apoptosis through mitophagy and ACSL4-mediated ferroptosis elicits anti-leukemia immunity","volume":"213","author":"Wei","year":"2024","journal-title":"Free Radic. Biol. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/S1470-2045(12)70137-7","article-title":"Global burden of cancers attributable to infections in 2008: A review and synthetic analysis","volume":"13","author":"Ferlay","year":"2012","journal-title":"Lancet Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"16431","DOI":"10.1002\/cam4.6283","article-title":"Alteration of gut microbiome and correlated amino acid metabolism are associated with acute myelocytic leukemia carcinogenesis","volume":"12","author":"Xu","year":"2023","journal-title":"Cancer Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rattanathammethee, T., Tuitemwong, P., Thiennimitr, P., Sarichai, P., Na Pombejra, S., Piriyakhuntorn, P., Hantrakool, S., Chai-Adisaksopha, C., Rattarittamrong, E., and Tantiworawit, A. (2020). Gut microbiota profiles of treatment-na\u00efve adult acute myeloid leukemia patients with neutropenic fever during intensive chemotherapy. PLoS ONE, 15.","DOI":"10.1101\/2020.07.09.194910"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wu, B., Xu, Y., Tang, M., Jiang, Y., Zhang, T., Huang, L., Wang, S., Hu, Y., Zhou, K., and Zhang, X. (2023). A Metabolome and Microbiome Analysis of Acute Myeloid Leukemia: Insights into the Carnosine-Histidine Metabolic Pathway. Toxics, 12.","DOI":"10.3390\/toxics12010014"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3194","DOI":"10.3324\/haematol.2023.284138","article-title":"Gut microbiome alterations at acute myeloid leukemia diagnosis are associated with muscle weakness and anorexia","volume":"109","author":"Havelange","year":"2024","journal-title":"Haematologica"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1002\/2211-5463.13193","article-title":"Profiling of gut microbial dysbiosis in adults with myeloid leukemia","volume":"11","author":"Yu","year":"2021","journal-title":"FEBS Open Bio"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Recharla, N., Geesala, R., and Shi, X.-Z. (2023). Gut Microbial Metabolite Butyrate and Its Therapeutic Role in Inflammatory Bowel Disease: A Literature Review. Nutrients, 15.","DOI":"10.20944\/preprints202304.0501.v1"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1182\/blood.2022018092","article-title":"Metabolism in acute myeloid leukemia: Mechanistic insights and therapeutic targets","volume":"141","author":"Mishra","year":"2023","journal-title":"Blood"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1182\/bloodadvances.2021006783","article-title":"Lasting shift in the gut microbiota in patients with acute myeloid leukemia","volume":"6","author":"Rashidi","year":"2022","journal-title":"Blood Adv."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhong, F.-L., He, J.-J., Bai, K.-H., Shao, R.-N., Wu, G.-Y., Tian, X.-P., Wang, D.-W., Dai, Y.-J., and Chen, S.-L. (2024). Tigecycline-induced coagulation gene prognostic prediction model and intestinal flora signature in AML. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1486592"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1800897","DOI":"10.1080\/19490976.2020.1800897","article-title":"Impact and consequences of intensive chemotherapy on intestinal barrier and microbiota in acute myeloid leukemia: The role of mucosal strengthening","volume":"12","author":"Hueso","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s00280-021-04385-0","article-title":"Curcumin sensitizes response to cytarabine in acute myeloid leukemia by regulating intestinal microbiota","volume":"89","author":"Liu","year":"2022","journal-title":"Cancer Chemother. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1182\/blood.2023021380","article-title":"CPX-351 exploits the gut microbiota to promote mucosal barrier function, colonization resistance, and immune homeostasis","volume":"143","author":"Renga","year":"2024","journal-title":"Blood"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1093\/cid\/ciz777","article-title":"Gut Microbiome Signatures Are Predictive of Infectious Risk Following Induction Therapy for Acute Myeloid Leukemia","volume":"71","author":"Shi","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e70501","DOI":"10.1002\/cam4.70501","article-title":"Gut Microbiome as a Potential Marker of Hematologic Recovery Following Induction Therapy in Acute Myeloid Leukemia Patients","volume":"14","author":"Salvestrini","year":"2025","journal-title":"Cancer Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Yadegar, A., Bar-Yoseph, H., Monaghan, T.M., Pakpour, S., Severino, A., Kuijper, E.J., Smits, W.K., Terveer, E.M., Neupane, S., and Nabavi-Rad, A. (2024). Fecal microbiota transplantation: Current challenges and future landscapes. Clin. Microbiol. Rev., 37.","DOI":"10.1128\/cmr.00060-22"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5306","DOI":"10.1200\/JCO.22.02366","article-title":"Randomized Double-Blind Phase II Trial of Fecal Microbiota Transplantation Versus Placebo in Allogeneic Hematopoietic Cell Transplantation and AML","volume":"41","author":"Rashidi","year":"2023","journal-title":"J. Clin. Oncol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1038\/s41467-021-23376-6","article-title":"Gut microbiota diversity after autologous fecal microbiota transfer in acute myeloid leukemia patients","volume":"12","author":"Malard","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhang, X., Ishikawa, D., Nomura, K., Fukuda, N., Haraikawa, M., Haga, K., Shibuya, T., Mita, T., and Nagahara, A. (2022). Donor Screening Revisions of Fecal Microbiota Transplantation in Patients with Ulcerative Colitis. J. Clin. Med., 11.","DOI":"10.3390\/jcm11041055"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1136\/gutjnl-2023-329948","article-title":"The first international Rome consensus conference on gut microbiota and faecal microbiota transplantation in inflammatory bowel disease","volume":"72","author":"Lopetuso","year":"2023","journal-title":"Gut"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1053\/j.gastro.2021.06.009","article-title":"Fecal Microbiota Transplantation Influences Procarcinogenic Escherichia coli in Recipient Recurrent Clostridioides difficile Patients","volume":"161","author":"Nooij","year":"2021","journal-title":"Gastroenterology"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/15\/9\/568\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:35:28Z","timestamp":1760034928000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/15\/9\/568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,25]]},"references-count":39,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["metabo15090568"],"URL":"https:\/\/doi.org\/10.3390\/metabo15090568","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,25]]}}}