{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:59:47Z","timestamp":1782921587138,"version":"3.54.5"},"reference-count":48,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science & Technology Innovation Program of Yongjiang 2035","award":["2024Z281"],"award-info":[{"award-number":["2024Z281"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: Chronic colitis presents a growing global health burden with rising incidence. This study investigated the ameliorative effect of Dendrobium officinale polysaccharide (DOP) against dextran sulfate sodium (DSS)-induced chronic colitis in mice, specifically examining its dual modulation of gut microbiota and metabolic pathways. Methods: DOP was extracted and purified from Dendrobium officinale stems and leaves. A chronic colitis model was established in male C57BL\/6J mice via DSS induction. Eighty-four mice were randomized into seven groups: control, model, low\/high-dose leaf-DOP, low\/high-dose stem-DOP, and sulfasalazine positive control. We assessed body weight, disease activity index (DAI), colon length, splenic\/thymic indices, inflammatory cytokines, and histopathology (Hematoxylin and Eosin\/Alcian blue staining), with tight junction protein and tumor necrosis factor-alpha (TNF-\u03b1) expression quantified via immunofluorescence. 16S rRNA sequencing and untargeted metabolomics evaluated microbial and metabolic shifts. Results: DOP significantly attenuated colitis severity, restored colon histoarchitecture, elevated goblet cell counts, upregulated zonula occludens-1 (ZO-1) and occludin expression, and suppressed TNF-\u03b1. Crucially, DOP remodeled dysbiosis by enriching beneficial taxa (e.g., Candidatus_Saccharimonas, Lachnoclostridium) while reducing pathogens (Mucispirillum). Metabolomics further elucidated DOP-mediated regulation of purine and nicotinate\/nicotinamide metabolism\u2014pathways mechanistically linked to its anti-inflammatory and barrier-repair effects. Conclusions: DOP effectively alleviates symptoms of DSS-induced chronic colitis in mice, protects intestinal barrier integrity, and achieves therapeutic potential through simultaneous regulation of the gut microbiome and metabolome.<\/jats:p>","DOI":"10.3390\/metabo15110708","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T05:28:43Z","timestamp":1761888523000},"page":"708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dendrobium officinale Polysaccharide Relieves the DSS-Induced Chronic Colitis in C57BL\/6J Mice and Regulates Colonic Microflora Structure"],"prefix":"10.3390","volume":"15","author":[{"given":"Yangyu","family":"Ma","sequence":"first","affiliation":[{"name":"National Key Laboratory for Development and Utilization of Forest Food Resources, The College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrui","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianling","family":"Yuan","sequence":"additional","affiliation":[{"name":"Institute of Functional Food, Zhejiang Dongri Food Science Research Center, Wenzhou 325006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Tao","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"Xing","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Development and Utilization of Forest Food Resources, The College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s10068-023-01367-1","article-title":"Sesame cake extract attenuates dextran sulfate sodium-induced colitis through inhibition of oxidative stress in mice","volume":"33","author":"Tungalag","year":"2024","journal-title":"Food Sci. 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