{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T09:42:18Z","timestamp":1762422138737,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42066006"],"award-info":[{"award-number":["42066006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Guangxi Natural Science Foundation","doi-asserted-by":"publisher","award":["2024GXNSFAA010403"],"award-info":[{"award-number":["2024GXNSFAA010403"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High-Level Talent Training Project Foundation of Guangxi University of Chinese Medicine","award":["202407","2022C011"],"award-info":[{"award-number":["202407","2022C011"]}]},{"name":"Guangxi First-class Discipline: Chinese Materia Medica","award":["[2022] No. 1"],"award-info":[{"award-number":["[2022] No. 1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: The search for environmentally friendly antifouling agents has led to an increased focus on marine natural products. Methods: This study investigated the antifouling potential of lipid fractions extracted from ten marine fungal strains isolated from the Beibu Gulf, China. The lipids were evaluated through a multi-level bioassay approach, including the inhibition of microfouling (against four fouling bacteria: Marinobacterium jannaschii, Vibrio pelagius, Vibrio rotiferianus, and Alteromonas macleodii), the prevention of macrofouling (inhibition of barnacle Amphibalanus reticulatus cyprid settlement), and long-term (90-day) marine field trials. Results: Eight lipid fractions demonstrated inhibitory effects against at least one bacterial strain. Five lipids significantly inhibited barnacle cyprid settlement, with half-maximal effective concentration (EC50) values ranging from 0.21 to 1.81 \u00b5g\/mL and exhibited low toxicity (half-maximal lethal concentration (LC50) &gt; 50 \u00b5g\/mL). Notably, four lipid fractions maintained potent antifouling efficacy (&gt;70% inhibition) throughout the 90-day field exposure. Chemical characterization via gas chromatography\u2013mass spectrometry (GC\u2013MS) revealed that the bioactive fractions were predominantly composed of fatty acids and their derivatives. Major identified compounds included palmitic acid, methyl palmitate, linoleic acid, dodecyl-9-ynyl chloroacetate, cis-13-octadecenoic acid, oleic acid, methyl 11,14-octadecadienoate, and (E)-9-octadecenoic acid methyl ester. Conclusions: This work represents the first comprehensive investigation of marine fungal lipids from the Beibu Gulf with multi-target antifouling properties, providing a theoretical foundation and practical candidate compounds for developing eco-friendly antifouling coatings.<\/jats:p>","DOI":"10.3390\/metabo15110721","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:06:06Z","timestamp":1762362366000},"page":"721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifouling Lipids from Marine Fungi of the Beibu Gulf"],"prefix":"10.3390","volume":"15","author":[{"given":"Mengfan","family":"Qi","sequence":"first","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0897-0073","authenticated-orcid":false,"given":"Wang","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Guangxi University, Nanning 530004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Su","sequence":"additional","affiliation":[{"name":"College of Agriculture, Guangxi University, Nanning 530004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-1609","authenticated-orcid":false,"given":"Xiaowei","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7524-8049","authenticated-orcid":false,"given":"Chenghai","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-3108","authenticated-orcid":false,"given":"Yonghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5968-6869","authenticated-orcid":false,"given":"Xinya","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Marine Drugs, Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"ref_1","first-page":"1241","article-title":"Antifouling natural products from marine fungi","volume":"40","author":"Chen","year":"2021","journal-title":"Mycosystema"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5230","DOI":"10.5846\/stxb201202130185","article-title":"A review on the balanomorph barnacles in the coastal waters of China","volume":"32","author":"Yan","year":"2012","journal-title":"Acta Ecol. Sin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1038\/s41589-024-01740-z","article-title":"Chemical signaling in biofilm-mediated biofouling","volume":"20","author":"Liu","year":"2024","journal-title":"Nat. Chem. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/08927014.2010.542809","article-title":"Economic impact of biofouling on a naval surface ship","volume":"27","author":"Schultz","year":"2011","journal-title":"Biofouling"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1080\/08927014.2017.1373279","article-title":"Effect of barnacle fouling on ship resistance and powering","volume":"33","author":"Demirel","year":"2017","journal-title":"Biofouling"},{"key":"ref_6","first-page":"37","article-title":"Research progress of nanomaterial-based coatings for marine antifouling applications","volume":"36","author":"He","year":"2023","journal-title":"Chin. Surf. Eng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.marpol.2015.11.015","article-title":"Costs and benefits to European shipping of ballast-water and hull-fouling treatment: Impacts of native and non-indigenous species","volume":"64","author":"Fernandes","year":"2016","journal-title":"Mar. Policy"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s00773-020-00720-x","article-title":"Recent developments of titanium dioxide materials for aquatic antifouling application","volume":"26","author":"Szeto","year":"2021","journal-title":"J. Mar. Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100889","DOI":"10.1016\/j.pmatsci.2021.100889","article-title":"Bioinspired marine antifouling coatings: Status, prospects, and future","volume":"124","author":"Jin","year":"2022","journal-title":"Prog. Mater. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Miftah, J.A., Hanif, N., Tan, L.T., Muljani, S., and Mubarik, N.R. (2025). The next generation of integrated eco-friendly anti-biofouling and anti-biocorrosion marine natural products. Chem. Biodivers.","DOI":"10.1002\/cbdv.202502263"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Varrella, S., Barone, G., Corinaldesi, C., Giorgetti, A., Nomaki, H., Nunoura, T., Rastelli, E., Tangherlini, M., Danovaro, R., and Dell\u2019Anno, A. (2024). Fungal abundance and diversity in the Mariana Trench, the deepest ecosystem on earth. J. Fungi, 16.","DOI":"10.3390\/jof10010073"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ghattavi, S., Homaei, A., and Fernandes, P. (2024). Marine natural products for biofouling elimination in marine environments. Biocatal. Agric. Biotechnol., 61.","DOI":"10.1016\/j.bcab.2024.103385"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s10295-014-1412-9","article-title":"Antifouling potentials of eight deep-sea-derived fungi from the South China Sea","volume":"41","author":"Zhang","year":"2014","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_14","first-page":"1210","article-title":"Marine natural products as antifouling molecules\u2014A mini-review (2014\u20132020)","volume":"36","author":"Liu","year":"2020","journal-title":"Biofouling"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Morgan, R.N., Ali, A.A., Alshahrani, M.Y., and Aboshanab, K.M. (2023). New insights on biological activities, chemical compositions, and classifications of marine actinomycetes antifouling agents. Microorganisms, 11.","DOI":"10.3390\/microorganisms11102444"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s11274-006-9220-7","article-title":"Isolation of antifouling compounds from the marine bacterium, Shewanella oneidensis SCH0402","volume":"23","author":"Bhattarai","year":"2007","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s11998-018-0110-3","article-title":"Alkyl 2-furoates obtained by green chemistry procedures as suitable new antifoulants for marine protective coatings","volume":"16","author":"Escobar","year":"2019","journal-title":"J. Coat. Technol. Res."},{"key":"ref_18","first-page":"30","article-title":"Study on the antibacterial activity of the extract of Melscope pielcifolia","volume":"53","author":"Chu","year":"2024","journal-title":"Shandong Chem. Ind."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Takamura, H., Yorisue, T., Tanaka, K., and Kadota, I. (2025). Antifouling activity of xylemin, its structural analogs, and related polyamines. Chem. Biodivers., 22.","DOI":"10.1002\/cbdv.202403213"},{"key":"ref_20","first-page":"6647","article-title":"An overview of fouling ascidians","volume":"37","author":"Yan","year":"2017","journal-title":"Acta Ecol. Sin."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"153638","DOI":"10.1016\/j.cej.2024.153638","article-title":"R Research progress on eco-friendly natural antifouling agents and their antifouling mechanisms","volume":"495","author":"Qiu","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.marpolbul.2011.01.012","article-title":"Antifouling strategies: History and regulation, ecological impacts and mitigation","volume":"62","author":"Dafforn","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jes.2017.03.040","article-title":"SeaNine 211 as antifouling biocide: A coastal pollutant of emerging concern","volume":"61","author":"Chen","year":"2017","journal-title":"J. Environ. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0160-4120(03)00176-4","article-title":"Worldwide occurrence and effects of antifouling paint booster biocides in the aquatic environment: A review","volume":"30","author":"Konstantinou","year":"2004","journal-title":"Environ. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1007\/s11802-014-2469-9","article-title":"Antifouling potential of bacteria isolated from a marine biofilm","volume":"13","author":"Gao","year":"2014","journal-title":"J. Ocean Univ. China"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.3390\/md12115503","article-title":"Inhibition of bacterial quorum sensing by extracts from aquatic fungi: First report from marine endophytes","volume":"12","author":"Reyes","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chen, L., and Qian, P.Y. (2017). Review on molecular mechanisms of antifouling compounds: An update since 2012. Mar. Drugs, 15.","DOI":"10.3390\/md15090264"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hao, R., Li, T., Zhou, X., Guo, W., Liu, J., Guo, L., and Wang, Y. (2025). Strategies and challenges in marine antifouling coatings: Current innovations and future outlook. J. Coat. Technol. Res.","DOI":"10.1007\/s11998-025-01123-6"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/15\/11\/721\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T09:38:39Z","timestamp":1762421919000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/15\/11\/721"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,5]]},"references-count":28,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["metabo15110721"],"URL":"https:\/\/doi.org\/10.3390\/metabo15110721","relation":{},"ISSN":["2218-1989"],"issn-type":[{"type":"electronic","value":"2218-1989"}],"subject":[],"published":{"date-parts":[[2025,11,5]]}}}