{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:12:46Z","timestamp":1791277966454,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["MINI.A070.22.004"],"award-info":[{"award-number":["MINI.A070.22.004"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wroc\u0142aw Medical University Biobank"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: This pilot study aimed to evaluate the metabolic profiles in plasma and cerebrospinal fluid (CSF) of 14 patients with acute lymphoblastic leukemia (ALL) and plasma of a control group, using proton magnetic resonance spectroscopy (1H NMR). Methods: Multivariate analysis, including orthogonal partial least-squares discriminant analysis (OPLS-DA), was used to analyze the metabolome composition. Results: Significant differences in plasma metabolic profiles were found between the ALL and control groups. We detected elevated levels of formate, citrate, and glycerophosphocholine (GPC), along with decreased concentrations of glutamine and myo-inositol. The OPLS-DA model showed stability, with R2Y = 69.7% and Q2 = 45.15%. Additionally, we observed differences in chemical shifts for leucine, myo-inositol, alanine, phenylalanine, and valine between CSF and plasma in patients with ALL. Conclusions: Our findings suggest that metabolomic analysis with 1H NMR is a promising tool for identifying potential molecular biomarkers and for deepening our understanding of metabolic reprogramming in pediatric ALL. The observed metabolic differences highlight the potential involvement of the central nervous system in the disease\u2019s pathophysiology.<\/jats:p>","DOI":"10.3390\/metabo16030160","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T08:51:57Z","timestamp":1772441517000},"page":"160","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["1H NMR-Based Metabolomics in Pediatric Acute Lymphoblastic Leukemia: A Pilot Study of Plasma and Cerebrospinal Fluid Profiles"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2826-6623","authenticated-orcid":false,"given":"Agata","family":"Serrafi","sequence":"first","affiliation":[{"name":"Department of Chemistry and Immunochemistry, Wroclaw Medical University, ul. M. Sklodowskiej-Curie 48\/50, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Pupek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Immunochemistry, Wroclaw Medical University, ul. M. Sklodowskiej-Curie 48\/50, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Lewandowski","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Wroclaw Medical University, ul. T. Cha\u0142ubi\u0144skiego 10, 50-368 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Janicka-K\u0142os","sequence":"additional","affiliation":[{"name":"Department of Basic Chemistry, Wroclaw Medical University, ul. Borowska 211A, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9908-6921","authenticated-orcid":false,"given":"Andrzej","family":"Wasilewski","sequence":"additional","affiliation":[{"name":"Student Scientific Association of Medical Chemistry and Immunochemistry, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Kasprzak","sequence":"additional","affiliation":[{"name":"Student Scientific Association of Medical Chemistry and Immunochemistry, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1064-1237","authenticated-orcid":false,"given":"Agnieszka","family":"Matera-Witkiewicz","sequence":"additional","affiliation":[{"name":"Wroclaw Medical University Biobank, Screening of Biological Activity Assays and Collection of Biological Material Laboratory, Wroclaw Medical University, ul. Borowska 211A, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-4806","authenticated-orcid":false,"given":"Tomasz","family":"Zato\u0144ski","sequence":"additional","affiliation":[{"name":"Department and Clinic of Otolaryngology, Head and Neck Surgery, Wroc\u0142aw Medical University, Borowska 213, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Po\u0142tyn-Zaradna","sequence":"additional","affiliation":[{"name":"Department of Social Medicine, Wroclaw Medical University, 50-345 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8976-6683","authenticated-orcid":false,"given":"Milena","family":"\u015aciskalska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biochemistry, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Brutkowski","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation, Pediatric Oncology and Hematology, Wroclaw Medical University, ul. Borowska 213, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernarda","family":"Kazanowska","sequence":"additional","affiliation":[{"name":"Department of Bone Marrow Transplantation, Pediatric Oncology and Hematology, Wroclaw Medical University, ul. Borowska 213, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,28]]},"reference":[{"key":"ref_1","first-page":"731","article-title":"Leukemia: An Overview for Primary Care","volume":"89","author":"Davis","year":"2014","journal-title":"Am. Fam. Physician"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"a034819","DOI":"10.1101\/cshperspect.a034819","article-title":"Epidemiology and Etiology of Leukemia and Lymphoma","volume":"10","author":"Bispo","year":"2020","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_3","unstructured":"(2025, December 18). Cancer Today. 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