{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:29:59Z","timestamp":1785407399774,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guizhou Provincial Science and Technology Foundation","award":["ZK[2025]642"],"award-info":[{"award-number":["ZK[2025]642"]}]},{"name":"Guizhou Provincial Health Commission Science and Technology Foundation","award":["gzwkj2024-348"],"award-info":[{"award-number":["gzwkj2024-348"]}]},{"name":"Guiyang Science and Technology Foundation","award":["[2023]13-17"],"award-info":[{"award-number":["[2023]13-17"]}]},{"award":["[2023]13-17"],"award-info":[{"award-number":["[2023]13-17"]}],"id":[{"id":"https:\/\/ror.org\/00gjmje40","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003459","name":"Guizhou University","doi-asserted-by":"crossref","award":["[2024]37"],"award-info":[{"award-number":["[2024]37"]}],"id":[{"id":"10.13039\/501100003459","id-type":"DOI","asserted-by":"crossref"}]},{"award":["[2024]37"],"award-info":[{"award-number":["[2024]37"]}],"id":[{"id":"https:\/\/ror.org\/02wmsc916","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: Dietary cholesterol intake significantly influences liver health, yet the specific molecular mechanisms by which it accelerates fibrogenesis remain incompletely defined. This study aimed to characterize the dose-dependent effects of dietary cholesterol on hepatic injury and fibrogenesis, identify cholesterol-responsive gene networks through transcriptomic analysis, and investigate Annexin A2 (ANXA2) as a candidate molecular mediator linking dietary cholesterol to hepatic fibrosis progression. Methods: A CCl4-induced liver fibrosis mouse model was established and supplemented with dietary cholesterol (1\u20132%). Liver injury and fibrosis were assessed by liver-to-body weight ratios, serum biochemical markers, histological analysis, and fibrogenic gene expression. RNA sequencing combined with multiple hepatic fibrosis database analyses was performed to identify potential molecular mediators. Results: Dietary cholesterol supplementation aggravated CCl4-induced hepatic fibrosis in mice, with dose-dependent increases in liver-to-body weight ratios and serum AST and ALT levels. Histological analysis showed enhanced collagen deposition and upregulation of fibrogenic genes. By integrating RNA-sequencing with multiple hepatic fibrosis database analysis and correlation analysis, we identified Annexin A2 (ANXA2) as a cholesterol-responsive gene associated with fibrosis. Conclusions: Dietary cholesterol promotes liver fibrosis progression, and ANXA2 may act as a potential mediator linking cholesterol metabolism to hepatic fibrogenesis.<\/jats:p>","DOI":"10.3390\/metabo16050331","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T14:19:29Z","timestamp":1778854769000},"page":"331","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Annexin A2 Is Associated with Dietary Cholesterol-Induced Metabolic Dysregulation and the Progression of Hepatic Fibrosis"],"prefix":"10.3390","volume":"16","author":[{"given":"Jiayang","family":"Liu","sequence":"first","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ou","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Tai","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghan","family":"Chai","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Tan","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Lin","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7715-2668","authenticated-orcid":false,"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cao","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guizhou University Medical College, Xiahui Road, Huaxi District, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/ajcn\/nqz066","article-title":"Egg consumption, cholesterol intake, and risk of incident stroke in men: The Kuopio Ischaemic Heart Disease Risk Factor Study","volume":"110","author":"Abdollahi","year":"2019","journal-title":"Am. 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