{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:22:17Z","timestamp":1785424937035,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"crossref","award":["5\/9\/1453\/22-Nut"],"award-info":[{"award-number":["5\/9\/1453\/22-Nut"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]},{"award":["5\/9\/1453\/22-Nut"],"award-info":[{"award-number":["5\/9\/1453\/22-Nut"]}],"id":[{"id":"https:\/\/ror.org\/0492wrx28","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Department of Higher Education"},{"id":[{"id":"https:\/\/ror.org\/0218hya98","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: Obesity is a complex disease involving the accumulation of an excessive amount of body fat. It is a condition that develops when energy intake and expenditure are out of balance. Inflammation and hypertrophy are caused by the storage of too much white adipose tissue, resulting in adiposity, which also secretes several pro-inflammatory cytokines. Several marketed drugs used to treat obesity have many side effects from long-term ingestion. Other therapeutic compounds from marine sources have already been established for treating obesity. In this paper, the main aim is to establish the anti-obesity effect of derived omega-3 fatty acids, i.e., 20:3(n-3)11-14-17 Icosa Trienoic Acid from Setipinna phasa oil. Methods: In the present investigation, inbred male Swiss albino mice were segregated into six categories as Control, Positive Control, Obese Control, and 20:3(n-3)11-14-17 Icosa Trienoic Acid treated groups with three different doses: Treatment 1, Treatment 2 and Treatment 3. To establish the potentiality of extracted fatty acid, different parameters would be considered, such as body weight, lipid composition and different obesity and obesity-associated inflammation markers. Results: After the isolated compound from Setipinna phasa oil was applied to the treated mice group, it decreased their body weight and serum lipid profile by 39.05%, 62.69%, 62.72%, and 78.46% compared to obese mice. They also had lower levels of uric acid, Serum Glutamic-Oxaloacetic Transaminase, Serum Glutamic Pyruvic Transaminase, and Alkaline Phosphatase, at 67.52%, 57.09%, 64.80%, and 43.99%, than the obese group. Accordingly, the treated group\u2019s expression of genes linked to obesity and pro-inflammatory cytokines was downregulated. The isolated compound affected both anti-inflammatory and anti-obesity markers\u2019 increased expression. Conclusions: After the experiments, it was found that the possibility of using fatty acids might be helpful as an anti-inflammatory and anti-obesity therapeutic strategy. This therapeutic strategy will be cheap and cost-effective.<\/jats:p>","DOI":"10.3390\/metabo16060384","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:11:01Z","timestamp":1780333861000},"page":"384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Icosa-11,14,17-Trienoic Acid from Setipinna phasa Oil on Lipogenesis and Adipose Inflammation on Mice with High Fat Diet Induced Obesity"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9941-5841","authenticated-orcid":false,"given":"Titli","family":"Panchali","sequence":"first","affiliation":[{"name":"Biodiversity and Environmental Studies Research Centre Affiliated to Vidyasagar University, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"},{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riya","family":"Kar","sequence":"additional","affiliation":[{"name":"Biodiversity and Environmental Studies Research Centre Affiliated to Vidyasagar University, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"},{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2553-6122","authenticated-orcid":false,"given":"Pipika","family":"Das","sequence":"additional","affiliation":[{"name":"Biodiversity and Environmental Studies Research Centre Affiliated to Vidyasagar University, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"},{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya","family":"Dutta","sequence":"additional","affiliation":[{"name":"Biodiversity and Environmental Studies Research Centre Affiliated to Vidyasagar University, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"},{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Phoujdar","sequence":"additional","affiliation":[{"name":"Biodiversity and Environmental Studies Research Centre Affiliated to Vidyasagar University, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"},{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4423-2471","authenticated-orcid":false,"given":"Kuntal","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrabani","family":"Pradhan","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Department of Paramedical and Allied Health Sciences, Midnapore City College, Bhadutala, Paschim Medinipur 721129, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.toxrep.2015.11.003","article-title":"Fish oil prevents excessive accumulation of subcutaneous fat caused by an adverse effect of pioglitazone treatment and positively changes adipocytes in KK mice","volume":"3","author":"Iizuka","year":"2016","journal-title":"Toxicol. Rep."},{"key":"ref_2","first-page":"125","article-title":"Obesity: An introduction and evaluation","volume":"1","author":"Gupta","year":"2011","journal-title":"J. Adv. Pharm. Educ. Res."},{"key":"ref_3","unstructured":"World Health Organization (2013, December 02). The Asia-Pacific Perspective: Redefining Obesity and Its Treatment. Available online: https:\/\/iris.who.int\/items\/a74d8440-5c1d-4237-9559-3f729058587b."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1038\/ijo.2008.102","article-title":"Global burden of obesity in 2005 and projections to 2030","volume":"32","author":"Kelly","year":"2008","journal-title":"Int. J. Obes."},{"key":"ref_5","first-page":"25","article-title":"Natural Product Ligands of the Peroxisome Proliferator-Activated Receptor Gamma as Anti-Inflammatory Mediators","volume":"13","author":"Derangula","year":"2023","journal-title":"Nat. Prod. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1161\/01.ATV.0000183883.72263.13","article-title":"A paracrine loop between adipocytes and macrophages aggravates inflammatory changes: Role of free fatty acids and tumor necrosis factor \u03b1","volume":"25","author":"Suganami","year":"2005","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.2174\/13816128113199990559","article-title":"A critical and comprehensive insight on heme oxygenase and related products including carbon monoxide, bilirubin, biliverdin and ferritin in type-1 and type-2 diabetes","volume":"20","author":"Mishra","year":"2014","journal-title":"Curr. Pharm. Des."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1073\/pnas.1133870100","article-title":"Monocyte chemoattractant protein 1 in obesity and insulin resistance","volume":"100","author":"Sartipy","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/B978-0-12-416003-3.00013-5","article-title":"Health benefits of n-3 polyunsaturated fatty acids: Eicosapentaenoic acid and docosahexaenoic acid","volume":"65","author":"Siriwardhana","year":"2012","journal-title":"Adv. Food Nutr. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e867","DOI":"10.1161\/CIR.0000000000000482","article-title":"Omega-3 polyunsaturated fatty acid (fish oil) supplementation and the prevention of clinical cardiovascular disease: A science advisory from the American Heart Association","volume":"135","author":"Siscovick","year":"2017","journal-title":"Circulation"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1499S","DOI":"10.1093\/ajcn\/83.6.1499S","article-title":"n \u2212 3 Fatty acids and the metabolic syndrome","volume":"83","author":"Carpentier","year":"2006","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"49","DOI":"10.32725\/jab.2024.003","article-title":"Amelioration of obesity induction by a high-fat diet and related inflammation by Phasa fish (Setipinna phasa) oil in BALB\/c mice","volume":"22","author":"Panchali","year":"2024","journal-title":"J. Appl. Biomed."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1101\/pdb.prot073411","article-title":"Manual hematoxylin and eosin staining of mouse tissue sections","volume":"2014","author":"Cardiff","year":"2014","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Andr\u00e9s-Manzano, M.J., Andr\u00e9s, V., and Dorado, B. (2015). Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root. Methods in Mouse Atherosclerosis, Springer.","DOI":"10.1007\/978-1-4939-2929-0_5"},{"key":"ref_15","unstructured":"Rasband, W. (2025, September 25). ImageJ. U.S. National Institutes of Health, Bethesda, Maryland, USA, Available online: http:\/\/imagej.nih.gov\/ij\/."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1152\/ajpgi.00291.2013","article-title":"Dissociation of hepatic insulin resistance from susceptibility of nonalcoholic fatty liver disease induced by a high-fat and high-carbohydrate diet in mice","volume":"306","author":"Asai","year":"2014","journal-title":"Am. J. Physiol.-Gastrointest. Liver Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12986-017-0188-0","article-title":"Eicosapentaenoic acid shows anti-inflammatory effect via GPR120 in 3T3-L1 adipocytes and attenuates adipose tissue inflammation in diet-induced obese mice","volume":"14","author":"Yamada","year":"2017","journal-title":"Nutr. Metab."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1172\/JCI20514","article-title":"Obesity-induced inflammatory changes in adipose tissue","volume":"112","author":"Wellen","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1161\/01.CIR.0000142055.53122.FA","article-title":"Circulating mononuclear cells in the obese are in a proinflammatory state","volume":"110","author":"Ghanim","year":"2004","journal-title":"Circulation"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1172\/JCI200319246","article-title":"Obesity is associated with macrophage accumulation in adipose tissue","volume":"112","author":"Weisberg","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1079\/NRR2006112","article-title":"Different dietary strategies for weight loss in obesity: Role of energy and macronutrient content","volume":"19","author":"Abete","year":"2006","journal-title":"Nutr. Res. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1007\/s11745-004-1345-9","article-title":"Omega-3 PUFA of marine origin limit diet-induced obesity in mice by reducing cellularity of adipose tissue","volume":"39","author":"Ruzickova","year":"2004","journal-title":"Lipids"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1139\/cjpp-2013-0050","article-title":"Metabolic inflammation: Role of cytokines in the crosstalk between adipose tissue and liver","volume":"91","author":"Gerner","year":"2013","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1007\/s11095-014-1526-9","article-title":"Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice","volume":"32","author":"Ma","year":"2015","journal-title":"Pharm. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.mam.2018.03.001","article-title":"Omega-3 fatty acids and non-alcoholic fatty liver disease: Evidence of efficacy and mechanism of action","volume":"64","author":"Scorletti","year":"2018","journal-title":"Mol. Asp. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Jo, J., Gavrilova, O., Pack, S., Jou, W., Mullen, S., Sumner, A.E., Cushman, S.W., and Periwal, V. (2009). Hypertrophy and\/or hyperplasia: Dynamics of adipose tissue growth. PLoS Comput. Biol., 5.","DOI":"10.1371\/journal.pcbi.1000324"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1089\/jmf.2020.0114","article-title":"Influence of omega-3 fatty acid-rich fish oils on hyperlipidemia: Effect of eel, sardine, trout, and cod oils on hyperlipidemic mice","volume":"24","author":"Kontostathi","year":"2021","journal-title":"J. Med. Food"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.3945\/jn.109.111567","article-title":"Dietary fish oil exerts hypolipidemic effects in lean and insulin sensitizing effects in obese LDLR\u2212\/\u2212 mice","volume":"139","author":"Saraswathi","year":"2009","journal-title":"J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6301","DOI":"10.1113\/JP272541","article-title":"Fish oil prevents changes induced by a high-fat diet on metabolism and adipokine secretion in mice subcutaneous and visceral adipocytes","volume":"594","author":"Crisma","year":"2016","journal-title":"J. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5556","DOI":"10.1038\/s41598-023-32851-7","article-title":"Polyunsaturated fatty acids-rich dietary lipid prevents high fat diet-induced obesity in mice","volume":"13","author":"Haneishi","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Vell, M.S., Creasy, K.T., Scorletti, E., Seeling, K.S., Hehl, L., Rendel, M.D., Schneider, K.M., and Schneider, C.V. (2023). Omega-3 intake is associated with liver disease protection. Front. Public Health, 11.","DOI":"10.3389\/fpubh.2023.1192099"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1007\/s00125-006-0300-x","article-title":"Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n \u2212 3 polyunsaturated fatty acids","volume":"49","author":"Todoric","year":"2006","journal-title":"Diabetologia"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Yamazaki, T., Li, D., and Ikaga, R. (2021). Fish oil increases diet-induced thermogenesis in mice. Mar. Drugs, 19.","DOI":"10.3390\/md19050278"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Antraco, V.J., Hirata, B.K.S., de Jesus Simao, J., Cruz, M.M., da Silva, V.S., da Cunha de Sa, R.D.C., Abdala, F.M., Armelin-Correa, L., and Alonso-Vale, M.I.C. (2021). Omega-3 polyunsaturated fatty acids prevent nonalcoholic steatohepatitis (NASH) and stimulate adipogenesis. Nutrients, 13.","DOI":"10.3390\/nu13020622"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"P\u00e9rez Lugo, M.I., Salas, M.L., Shrestha, A., and Ramalingam, L. (2024). Fish Oil Improves Offspring Metabolic Health of Paternal Obese Mice by Targeting Adipose Tissue. Biomolecules, 14.","DOI":"10.3390\/biom14040418"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"674","DOI":"10.5551\/jat.1313","article-title":"Anti-obesity effect of fish oil and fish oil-fenofibrate combination in female KK mice","volume":"16","author":"Arai","year":"2009","journal-title":"J. Atheroscler. Thromb."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1093\/jn\/nxz215","article-title":"Increasing dietary fish oil reduces adiposity and mitigates bone deterioration in growing C57BL\/6 mice fed a high-fat diet","volume":"150","author":"Cao","year":"2020","journal-title":"J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"355","DOI":"10.3390\/nu2030355","article-title":"Omega-3 fatty acids and inflammatory processes","volume":"2","author":"Calder","year":"2010","journal-title":"Nutrients"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.ecl.2008.06.007","article-title":"Obesity and free fatty acids","volume":"37","author":"Boden","year":"2008","journal-title":"Endocrinol. Metab. Clin. N. Am."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ahmadi, A.R., Shirani, F., Abiri, B., Siavash, M., Haghighi, S., and Akbari, M. (2023). Impact of omega-3 fatty acids supplementation on the gene expression of peroxisome proliferator activated receptors-\u03b3, \u03b1 and fibroblast growth factor-21 serum levels in patients with various presentation of metabolic conditions: A GRADE assessed systematic review and dose\u2013response meta-analysis of clinical trials. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1202688"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/6\/384\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:41:05Z","timestamp":1780548065000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/6\/384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["metabo16060384"],"URL":"https:\/\/doi.org\/10.3390\/metabo16060384","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]}}}