{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:08:40Z","timestamp":1793725720431,"version":"4.6.1"},"reference-count":79,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360090"],"award-info":[{"award-number":["32360090"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32360090"],"award-info":[{"award-number":["32360090"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Guangxi","award":["2025GXNSFHA069179"],"award-info":[{"award-number":["2025GXNSFHA069179"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2023GXNSFAA026289"],"award-info":[{"award-number":["2023GXNSFAA026289"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2026GXNSFHA00640321"],"award-info":[{"award-number":["2026GXNSFHA00640321"]}]},{"name":"Baise City \u201cSci-Tech Platform Building\u201d Special Action","award":["Baike LT252805"],"award-info":[{"award-number":["Baike LT252805"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Obesity is closely associated with gut microbiota dysbiosis. Nervonic acid (NA; (15Z)-15-tetracosenoic acid) is a bioactive fatty acid with reported metabolic effects. This study aimed to investigate the associations between NA administration, gut microbiota composition changes, and host metabolic phenotypes in high-fat diet (HFD)-fed mice. Methods: C57BL\/6J mice were fed an HFD for 12 weeks and concurrently administered NA at doses of 20, 40, and 60 mg\/(kg\u00b7d) by gavage. Metabolic parameters, histopathological changes, and fecal microbiota composition (via 16S rRNA gene sequencing) were evaluated. Results: NA administration was associated with significantly attenuated HFD-induced increases in body weight and adipose tissue mass, as well as marked reductions in serum total cholesterol, triglycerides, and low-density lipoprotein cholesterol (all p &lt; 0.05). Hepatic steatosis and adipose tissue inflammation were also attenuated. 16S rRNA gene sequencing revealed that NA was associated with the counteraction of HFD-induced gut microbiota dysbiosis, including alterations in \u03b1-diversity and community structure. NA was associated with higher relative abundances of taxa such as Blautia, Oscillibacter, Faecalibaculum, Parabacteroides, Dubosiella, and Odoribacter and lower relative abundances of Lachnoclostridium, Mucispirillum, and Alistipes. Within-group correlation analyses showed that genera with higher relative abundances were inversely associated with lipid parameters and adiposity, whereas genera with lower relative abundances correlated positively with these metabolic indicators. Conclusions: NA administration was associated with bidirectional changes in gut microbiota composition\u2014the enrichment of certain taxa and the suppression of others\u2014concomitant with the amelioration of HFD-induced metabolic dysfunction. These findings indicate correlations between NA, gut microbiota alterations, and improved metabolic phenotypes; however, causality remains to be established.<\/jats:p>","DOI":"10.3390\/metabo16060399","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T16:25:16Z","timestamp":1780935916000},"page":"399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nervonic Acid Prevents HFD-Induced Metabolic Dysfunction and Is Associated with Gut Microbiota Remodeling"],"prefix":"10.3390","volume":"16","author":[{"given":"Cheng-Yu","family":"Jiang","sequence":"first","affiliation":[{"name":"School of Medical Technology and Artificial Intelligence, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5376-2737","authenticated-orcid":false,"given":"Zong-Liang","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Artificial Intelligence, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Food Inspection, Guangxi-Asean Food Inspection Center, Nanning 530022, China"},{"name":"Technology Innovation Center of Natural Fragrances and Flavors, State Administration for Market Regulation, Nanning 530022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Rong","family":"Cen","sequence":"additional","affiliation":[{"name":"Bai Se Shi Bai Lin Lin Chang, Baise 533099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong-Min","family":"Lu","sequence":"additional","affiliation":[{"name":"Baise Forestry Research Institute, Baise 533021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong-Bin","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Artificial Intelligence, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Yang","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Artificial Intelligence, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Jiang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Artificial Intelligence, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/dom.16263","article-title":"An Overview of Obesity-Related Complications: The Epidemiological Evidence Linking Body Weight and Other Markers of Obesity to Adverse Health Outcomes","volume":"27","year":"2025","journal-title":"Diabetes Obes. 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