{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:58:48Z","timestamp":1785419928203,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["B.SUB.26.391"],"award-info":[{"award-number":["B.SUB.26.391"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["B.SUB.26.397"],"award-info":[{"award-number":["B.SUB.26.397"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]},{"award":["B.SUB.26.391"],"award-info":[{"award-number":["B.SUB.26.391"]}],"id":[{"id":"https:\/\/ror.org\/00y4ya841","id-type":"ROR","asserted-by":"publisher"}]},{"award":["B.SUB.26.397"],"award-info":[{"award-number":["B.SUB.26.397"]}],"id":[{"id":"https:\/\/ror.org\/00y4ya841","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Lipoproteins are molecules composed of phospholipids and apolipoproteins that transport triacylglycerol and cholesterol in blood and are implicated in the development of many diseases. Methods: In this study, we fill a knowledge gap by precisely characterizing the apolipoprotein profile (with Bio-Plex Human Apolipoprotein Assay) in three metabolically separate groups of individuals (lean individuals and obese individuals without and with metabolic syndrome) and at four distinct time points (0, 3, 6, and 12 months post bariatric surgery). Results: Obese patients had a higher baseline ApoB\/ApoA1 ratio, which returned to the reference level over the follow-up period. The above is of clinical importance, as the ratio is a predictor of adverse cardiovascular events common in obese subjects. Interestingly, plasma concentrations of most of the investigated apolipoproteins appeared to be relatively stable at the onset of the experiment, with changes observed later in time. We detected significant drops in the levels of ApoC3, ApoD, and ApoH that occurred as early as three months post intervention. On the other hand, the levels of ApoA2, ApoE, and ApoJ increased with time. Of the above, ApoE is known to be involved in the removal of TAGs and cholesterol from the blood. Conversely, ApoJ appears to be one of the determinants of the tissues\u2019 responsiveness to insulin. Thus, it may be an indicator of the reduced insulin resistance observed a few months after the surgery. Conclusions: Overall, the investigated proteins have a documented role in the development and progression of vascular pathologies. Hence, our results may be interpreted as a sign of improved cardiovascular fitness of our patients.<\/jats:p>","DOI":"10.3390\/metabo16070496","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:05:58Z","timestamp":1784077558000},"page":"496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Beneficial Changes in Apolipoprotein Concentrations After Bariatric Surgery in Obese Women"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4954-3431","authenticated-orcid":false,"given":"Bart\u0142omiej","family":"\u0141ukaszuk","sequence":"first","affiliation":[{"name":"Department of Physiology, Medical University of Bialystok, 15-222 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7407-8156","authenticated-orcid":false,"given":"Adrian","family":"Chabowski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical University of Bialystok, 15-222 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Ziemba","sequence":"additional","affiliation":[{"name":"Clinical and Research Department of Applied Physiology, Mossakowski Medical Research Centre, Polish Academy of Science, 02-106 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Choroma\u0144ska","sequence":"additional","affiliation":[{"name":"1st Clinical Department of General and Endocrine Surgery, Medical University of Bialystok, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"My\u015bliwiec","sequence":"additional","affiliation":[{"name":"Department of Minimally Invasive Surgery, Medical University of Bialystok, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0945-9964","authenticated-orcid":false,"given":"Katarzyna","family":"Supruniuk","sequence":"additional","affiliation":[{"name":"Department of Biology and Medical Genetics, Medical University of Gda\u0144sk, 80-210 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4936-3120","authenticated-orcid":false,"given":"Agnieszka","family":"Mik\u0142osz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical University of Bialystok, 15-222 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/s41569-022-00676-y","article-title":"Metabolism of Triglyceride-Rich Lipoproteins in Health and Dyslipidaemia","volume":"19","author":"Taskinen","year":"2022","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e476","DOI":"10.1002\/mco2.476","article-title":"Cholesterol Metabolism: Physiological Regulation and Diseases","volume":"5","author":"Guo","year":"2024","journal-title":"MedComm"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1001\/jama.290.17.2292","article-title":"Effect of Recombinant Apoa-I Milano on Coronary Atherosclerosis in Patients with Acute Coronary Syndromes: A Randomized Controlled Trial","volume":"290","author":"Nissen","year":"2003","journal-title":"JAMA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100885","DOI":"10.1016\/j.jlr.2025.100885","article-title":"Exchangeable Apoa1 on Hdl Inhibits Ldl Binding to Proteoglycans","volume":"66","author":"Geh","year":"2025","journal-title":"J. Lipid Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8236325","DOI":"10.1155\/2017\/8236325","article-title":"Apolipoproteins a-I, B, and c-Iii and Obesity in Young Adult Cherokee","volume":"2017","author":"Wang","year":"2017","journal-title":"J. Lipids"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117359","DOI":"10.1016\/j.cca.2023.117359","article-title":"Role of Apolipoproteins in the Pathogenesis of Obesity","volume":"545","author":"Huang","year":"2023","journal-title":"Clin. Chim. Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s42000-018-0051-3","article-title":"Metabolic Syndrome: An Update on Diagnostic Criteria, Pathogenesis, and Genetic Links","volume":"17","author":"Zafar","year":"2018","journal-title":"Hormones"},{"key":"ref_8","first-page":"191","article-title":"World Medical Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects","volume":"310","author":"Association","year":"2013","journal-title":"JAMA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/1742-4682-9-46","article-title":"Dynamics of Glucose and Insulin Concentration Connected to the \u03b2-Cell Cycle: Model Development and Analysis","volume":"9","author":"Gallenberger","year":"2012","journal-title":"Theor. Biol. Med. Model."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4610834","DOI":"10.1155\/2022\/4610834","article-title":"Apolipoprotein A1-Related Proteins and Reverse Cholesterol Transport in Antiatherosclerosis Therapy: Recent Progress and Future Perspectives","volume":"2022","author":"Xu","year":"2022","journal-title":"Cardiovasc. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S121","DOI":"10.1016\/j.ihj.2023.12.001","article-title":"Apolipoprotein B\u2014An Ideal Biomarker for Atherosclerosis?","volume":"76","author":"Singh","year":"2024","journal-title":"Indian Heart J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/j.1365-2796.2006.01643.x","article-title":"The apoB\/apoA-I Ratio: A Strong, New Risk Factor for Cardiovascular Disease and a Target for Lipid-Lowering Therapy\u2013a Review of the Evidence","volume":"259","author":"Walldius","year":"2006","journal-title":"J. Intern. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s00392-025-02767-5","article-title":"Association of Apolipoprotein B, Excess Apolipoprotein B and apoB\/apoA1 Ratio with 20-Year Atherosclerotic Cardiovascular Disease Risk: The Attica Study (2002\u20132022)","volume":"115","author":"Giannakopoulou","year":"2026","journal-title":"Clin. Res. Cardiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0140-6736(04)17018-9","article-title":"Effect of Potentially Modifiable Risk Factors Associated with Myocardial Infarction in 52 Countries (the Interheart Study): Case-Control Study","volume":"364","author":"Yusuf","year":"2004","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"14","DOI":"10.7555\/JBR.33.20190048","article-title":"Pleiotropic Effects of Apolipoprotein a-II on High-Density Lipoprotein Functionality, Adipose Tissue Metabolic Activity and Plasma Glucose Homeostasis","volume":"34","author":"Zvintzou","year":"2020","journal-title":"J. Biomed. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"643","DOI":"10.2337\/diabetes.50.3.643","article-title":"Studies with Apolipoprotein a-II Transgenic Mice Indicate a Role for Hdls in Adiposity and Insulin Resistance","volume":"50","author":"Castellani","year":"2001","journal-title":"Diabetes"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1126\/science.8332912","article-title":"Atherosclerosis in Transgenic Mice Overexpressing Apolipoprotein a-II","volume":"261","author":"Warden","year":"1993","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"31532","DOI":"10.1038\/srep31532","article-title":"Bioinformatic Analysis of Plasma Apolipoproteins a-I and a-II Revealed Unique Features of a-I\/a-II Hdl Particles in Human Plasma","volume":"6","author":"Kido","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2524105","DOI":"10.1080\/10641963.2025.2524105","article-title":"Changes and Significance of High-Density Lipoprotein a-I, a-II, and Serum Amyloid Levels in Patients with Chronic Obstructive Pulmonary Disease Complicated by Coronary Heart Disease","volume":"47","author":"Tian","year":"2025","journal-title":"Clin. Exp. Hypertens."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/s41366-024-01549-9","article-title":"Insights into the Roles of Apolipoprotein E in Adipocyte Biology and Obesity","volume":"48","author":"Jiang","year":"2024","journal-title":"Int. J. Obes."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2337\/db07-0403","article-title":"Defective Lipid Delivery Modulates Glucose Tolerance and Metabolic Response to Diet in Apolipoprotein E-Deficient Mice","volume":"57","author":"Hofmann","year":"2008","journal-title":"Diabetes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1038\/s41467-020-15963-w","article-title":"Apolipoprotein J Is a Hepatokine Regulating Muscle Glucose Metabolism and Insulin Sensitivity","volume":"11","author":"Seo","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/cen.70087","article-title":"Dynamics of Apolipoprotein J Levels and Metabolic Parameters Following Bariatric Surgery","volume":"105","author":"Rodrigues","year":"2025","journal-title":"Clin. Endocrinol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.tem.2024.02.016","article-title":"A Unified Model for Regulating Lipoprotein Lipase Activity","volume":"35","author":"Zhang","year":"2024","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1186\/s12944-021-01605-7","article-title":"CRISPR\/Cas9-Mediated Knockout of Apoc3 Stabilizes Plasma Lipids and Inhibits Atherosclerosis in Rabbits","volume":"20","author":"Zha","year":"2021","journal-title":"Lipids Health Dis."},{"key":"ref_26","first-page":"310","article-title":"Expression and Prognostic Significance of Apolipoprotein d in Breast Cancer","volume":"144","author":"Vizoso","year":"1994","journal-title":"Am. J. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1007\/s11064-019-02859-5","article-title":"Hippocampal up-Regulation of Apolipoprotein d in a Rat Model of Maternal Hypo- and Hyperthyroidism: Implication of Oxidative Stress","volume":"44","author":"Salami","year":"2019","journal-title":"Neurochem. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"E802","DOI":"10.1152\/ajpendo.90725.2008","article-title":"Human Apolipoprotein d Overexpression in Transgenic Mice Induces Insulin Resistance and Alters Lipid Metabolism","volume":"296","author":"Fournier","year":"2009","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Labrie, M., Lalonde, S., Najyb, O., Thiery, M., Daneault, C., Des Rosiers, C., Rassart, E., and Mounier, C. (2015). Apolipoprotein d Transgenic Mice Develop Hepatic Steatosis Through Activation of Ppar\u03b3 and Fatty Acid Uptake. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0130230"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.atherosclerosis.2021.12.011","article-title":"von Elevated Levels of Apolipoprotein d Predict Poor Outcome in Patients with Suspected or Established Coronary Artery Disease","volume":"341","author":"Annema","year":"2022","journal-title":"Atherosclerosis"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.18632\/aging.100004","article-title":"Apolipoprotein d in Lipid Metabolism and Its Functional Implication in Atherosclerosis and Aging","volume":"1","author":"Perdomo","year":"2009","journal-title":"Aging"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.atherosclerosis.2009.09.072","article-title":"APOH Is Increased in the Plasma and Liver of Type 2 Diabetic Patients with Metabolic Syndrome","volume":"209","author":"Castro","year":"2010","journal-title":"Atherosclerosis"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s12944-022-01699-7","article-title":"Alcohol-Dependent Downregulation of Apolipoprotein H Exacerbates Fatty Liver and Gut Microbiota Dysbiosis in Mice","volume":"21","author":"Liu","year":"2022","journal-title":"Lipids Health Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1191\/0961203305lu2211oa","article-title":"Oxidized Ldl\/Beta2-Glycoprotein I Complexes: New Aspects in Atherosclerosis","volume":"14","author":"Matsuura","year":"2005","journal-title":"Lupus"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.amjcard.2016.06.023","article-title":"Oxidized Low-Density Lipoprotein-\u03b22-Glycoprotein I Complex but Not Free Oxidized Ldl Is Associated with the Presence and Severity of Coronary Artery Disease","volume":"118","author":"Bliden","year":"2016","journal-title":"Am. J. Cardiol."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/7\/496\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:22:36Z","timestamp":1784078556000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/7\/496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["metabo16070496"],"URL":"https:\/\/doi.org\/10.3390\/metabo16070496","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]}}}