{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:11:18Z","timestamp":1785859878103,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: Date seeds contain a wide range of biologically active constituents and have been associated with multiple health benefits. Our study aimed to evaluate the utility of biphasic aqueous methanol\/chloroform extraction for separating diverse metabolites from powdered Malakabi Ajwa date seeds and to identify the most effective chromatographic and spectroscopic techniques for their comprehensive profiling and structural characterization. Methods: Biphasic extraction generated organic, aqueous, and insoluble phases. Proper chromatography-mass spectrometry and spectroscopy analytical tools were then utilized to profile the metabolites in the aqueous and organic phases. Results and Conclusions: In the organic phase, fatty acids and their methyl esters were identified using GC-MS and confirmed by 1H NMR, while the absence of an O\u2013H band in the FT-IR spectrum, together with 1H NMR data, supported the natural esterification of these fatty acids. In the aqueous phase, UV-Vis spectroscopy and LC-MS revealed anthocyanin pigments, and LC-MS additionally detected four amino acids. HPLC was used to quantify and validate the sugar composition, and 1H 1D and 2D NMR spectra enabled the assignment of \u03b1- and \u03b2-glucopyranose, \u03b2-fructopyranose, and sucrose signals. 31P NMR indicated that orthophosphate monoesters were the predominant phosphorus-containing compounds in both organic and aqueous phases. Overall, our findings show how an integrated chromatographic and spectroscopic workflow provides a robust strategy for comprehensive metabolite profiling of natural products such as Malakabi Ajwa date seeds.<\/jats:p>","DOI":"10.3390\/metabo16080552","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T15:08:09Z","timestamp":1785856089000},"page":"552","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advantages of a Multi-Platform Workflow Integrating Biphasic Extraction, Chromatography, and NMR for Characterization of Metabolites in Extracts of Malakabi Ajwa Date Seeds"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8737-8417","authenticated-orcid":false,"given":"Mufeed M.","family":"Basti","sequence":"first","affiliation":[{"name":"Department of Chemistry, North Carolina A&T State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanelle","family":"Flowers","sequence":"additional","affiliation":[{"name":"Reynolds Tobacco Company, Winston Salem, NC 27105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anika R.","family":"Barnes","sequence":"additional","affiliation":[{"name":"The University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0886-5204","authenticated-orcid":false,"given":"Leonard L.","family":"Williams","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, NC Agricultural and Technical State University, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Todd","sequence":"additional","affiliation":[{"name":"Procter & Gamble, Mason, OH 45040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9034-3554","authenticated-orcid":false,"given":"Kiran","family":"Subedi","sequence":"additional","affiliation":[{"name":"Analytical Services Laboratory, North Carolina A&T State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0193-891X","authenticated-orcid":false,"given":"Amira A.","family":"Ayad","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, NC Agricultural and Technical State University, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ayad, A.A., Williams, L.L., El-Rab, D.A.G., Ayivi, R., Colleran, H.L., Aljaloud, S., and Ibrahim, S.A. 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