{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T04:32:15Z","timestamp":1786509135598,"version":"build-2736575974"},"reference-count":149,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002865","name":"Chongqing Municipal Science and Technology Bureau","doi-asserted-by":"crossref","award":["CSTB2024NSCQ-MSX0502"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0502"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"crossref"}]},{"award":["CSTB2024NSCQ-MSX0502"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0502"]}],"id":[{"id":"https:\/\/ror.org\/03ze5ke63","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007957","name":"Chongqing Municipal Education Commission","doi-asserted-by":"publisher","award":["KJQN202300518"],"award-info":[{"award-number":["KJQN202300518"]}],"id":[{"id":"10.13039\/501100007957","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007957","name":"Chongqing Municipal Education Commission","doi-asserted-by":"publisher","award":["KJQN202500527"],"award-info":[{"award-number":["KJQN202500527"]}],"id":[{"id":"10.13039\/501100007957","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Brassica crops, encompassing globally important vegetables and oilseeds, face severe threats from diverse biotic and abiotic stresses. Plant secondary metabolites constitute the chemical foundation of defence, and metabolomics has emerged as an effective systems biology tool for comprehensively dissecting stress-induced metabolic changes. Recent progress in applying metabolomics to elucidate defence mechanisms in Brassica crops is systematically synthesised here. Major stresses confronting Brassica crop production and the metabolic basis of plant defence are first outlined. Current analytical platforms, including liquid chromatography\u2013mass spectrometry, gas chromatography\u2013mass spectrometry, ion mobility spectrometry, and mass spectrometry imaging, are critically evaluated alongside data processing workflows and multi-omics integration strategies. Key defence-related metabolite classes identified in Brassica crops, notably glucosinolates (GSLs) and their hydrolysis products, phenolic compounds, and lipid-derived signalling molecules, are surveyed with emphasis on their respective functions in biotic and abiotic stress responses. Metabolomics has been instrumental in revealing distinct metabolic reprogramming patterns triggered by diverse stresses, including pathogen infection, insect herbivory, drought, salinity, temperature extremes, and heavy metal stress. Metabolomics-informed crop improvement strategies, including marker-assisted breeding, genetic and metabolic engineering, and precision agronomic practices, are discussed together with current technical bottlenecks and future directions involving artificial intelligence, metabolic modelling, and spatial metabolomics. The compiled knowledge provides a comprehensive reference for leveraging metabolomics to enhance stress resilience and sustainable production of Brassica crops.<\/jats:p>","DOI":"10.3390\/metabo16080563","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:24:53Z","timestamp":1786353893000},"page":"563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of Metabolomics in Defence Responses of Brassica Crops"],"prefix":"10.3390","volume":"16","author":[{"given":"Yufei","family":"Li","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Plant Environmental Adaptations Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxing","family":"Lu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Environmental Adaptations Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s11032-024-01514-4","article-title":"Zheyouza 59, a Rapeseed Variety with Superior Resistance and Excellent Yield","volume":"44","author":"Fu","year":"2024","journal-title":"Mol. 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