{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:24:26Z","timestamp":1787142266793,"version":"build-2736575974"},"reference-count":24,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82322029"],"award-info":[{"award-number":["82322029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32541024"],"award-info":[{"award-number":["32541024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82322029"],"award-info":[{"award-number":["82322029"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32541024"],"award-info":[{"award-number":["32541024"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder influenced by environmental factors including cold stress. While cold exposure exacerbates intestinal inflammation, the specific microbial metabolites linking environmental stress to colitis remain unclear. 3-Hydroxypropionate (3-HPA) is a gut microbial metabolite elevated following cold exposure, but its pathogenic role in intestinal inflammation has not been investigated. This study aimed to determine whether 3-HPA contributes to colitis progression and to characterize its effects on gut microbiota and intestinal epithelial function. Methods: We employed a dextran sulfate sodium (DSS)-induced colitis mouse model to assess the impact of cold exposure and exogenous 3-HPA administration. Paired shotgun metagenomic and metabolomic analyses were performed to evaluate gut microbial composition and metabolic outputs. Mechanistic studies using NCM460 intestinal epithelial cells were conducted to examine mitochondrial respiration and tight junction integrity under nutrient-limited conditions. Results: Cold exposure increased fecal 3-HPA levels and aggravated DSS-induced colitis, characterized by enhanced weight loss, histological damage, and immune cell infiltration. Direct 3-HPA supplementation alone was sufficient to worsen colitis severity. Multi-omics profiling revealed that 3-HPA reshaped gut microbiota composition, depleted short-chain fatty acids (SCFAs), and disrupted microbial tryptophan and bile acid metabolism. In vitro, 3-HPA impaired mitochondrial oxidative phosphorylation, reduced ATP production, and compromised tight junction organization in intestinal epithelial cells. Conclusions: These findings identify 3-HPA as a gut microbial metabolite elevated by cold exposure that contributes to colitis progression by disrupting beneficial microbial metabolism while also impairing epithelial mitochondrial function and barrier integrity. Modulating 3-HPA production or its downstream epithelial effects may represent a potential therapeutic approach for IBD exacerbated by environmental stress.<\/jats:p>","DOI":"10.3390\/metabo16080592","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:04:12Z","timestamp":1787137452000},"page":"592","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cold-Induced Elevation of 3-Hydroxypropionate Exacerbates Colitis by Remodeling Gut Microbiota and Impairing Mitochondrial Respiration in Intestinal Epithelial Cells"],"prefix":"10.3390","volume":"16","author":[{"given":"Yankun","family":"Jia","sequence":"first","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Xi\u2019an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baodong","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Xi\u2019an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kefei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine 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Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Xi\u2019an 710032, China"},{"name":"Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi\u2019an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Xi\u2019an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Xi\u2019an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"National Infrastructure for Translational Medicine (Xi\u2019an), Xi\u2019an 710032, China"},{"name":"State Key Laboratory of New Targets Discovery and Drug Development for Major 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