{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:18:23Z","timestamp":1787980703865,"version":"build-2784847793"},"reference-count":42,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Forestry Science and Technology Promotion Demonstration Fund Project","award":["Wan [2025] TG01"],"award-info":[{"award-number":["Wan [2025] TG01"]}]},{"DOI":"10.13039\/501100013142","name":"Key Research and Development Project of Hainan Province","doi-asserted-by":"crossref","award":["ZDYF2024SHFZ076"],"award-info":[{"award-number":["ZDYF2024SHFZ076"]}],"id":[{"id":"10.13039\/501100013142","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ecological Science and Technology Commissioner Project of the Science, Industry and Information Technology Bureau of Wuzhishan City, Hainan Province","award":["WZSKTPXM202210"],"award-info":[{"award-number":["WZSKTPXM202210"]}]},{"name":"Anhui Province Outstanding Young Teachers Training Program for Universities","award":["YQYB2024055"],"award-info":[{"award-number":["YQYB2024055"]}]},{"name":"High\u2013level Talents Research Initiation Funding Project","award":["WGKQ2022029"],"award-info":[{"award-number":["WGKQ2022029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Dendrobium huoshanense flowers are a potentially valuable medicinal resource, but metabolic variation among different germplasm materials remains incompletely characterized. This study aimed to characterize metabolic and transcriptional differences among three D. huoshanense germplasm materials and to explore gene\u2013metabolite associations related to phenylpropanoid metabolism. Methods: Untargeted LC\u2013MS metabolomics and transcriptome sequencing were used to comparatively profile flowers of three D. huoshanense germplasm materials (DH-1, DH-2, and DH-3). Results: Metabolomic profiling detected 4357 metabolic features putatively assigned to 12 chemical classes and revealed clear separation among the three materials. A total of 1292, 1861, and 2035 differentially accumulated metabolic features were identified in DH-1 vs. DH-2, DH-1 vs. DH-3, and DH-2 vs. DH-3, respectively. Transcriptome analysis identified 33,665 expressed genes, including 3365, 5244, and 5964 DEGs in the respective pairwise comparisons. Phenylpropanoid biosynthesis was recurrently enriched across all three DEG comparisons. Exploratory gene\u2013metabolite analysis identified associations involving phenylpropanoid\u2013related candidate genes, including PAL, C4H, 4CL\/4CL\u2013like, HCT\u2013like, CSE\u2013like, COMT\/OMT\u2013like, and CAD, with the DH-2 vs. DH-3 comparison showing the most extensive molecular differences. Conclusions: The three D. huoshanense germplasm materials exhibited distinct metabolic and transcriptional profiles, providing candidate genes and metabolic features for subsequent targeted validation.<\/jats:p>","DOI":"10.3390\/metabo16090612","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:02:27Z","timestamp":1787817747000},"page":"612","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Metabolomic and Transcriptomic Analyses Reveal Phenylpropanoid\u2013Associated Variation Among Three Dendrobium huoshanense Germplasm Materials"],"prefix":"10.3390","volume":"16","author":[{"given":"Peipei","family":"Wei","sequence":"first","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Du","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anhui Dabieshan Huohu Technology Co., Ltd., Lu\u2019an 237200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Dou","sequence":"additional","affiliation":[{"name":"Anhui Dabieshan Huohu Technology Co., Ltd., Lu\u2019an 237200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Dai","sequence":"additional","affiliation":[{"name":"Engineering Technology Research Center of Plant Cell Engineering, Lu\u2019an 237012, China"},{"name":"College of Life and Health, West Anhui University, Lu\u2019an 237012, China"},{"name":"Anhui Dabieshan Huohu Technology Co., Ltd., Lu\u2019an 237200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gao, L., Wang, F., Hou, T., Geng, C., Xu, T., Han, B., and Liu, D. 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