{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:18:49Z","timestamp":1787980729175,"version":"build-2784847793"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&amp;D Program of China","award":["2023YFF0713900"],"award-info":[{"award-number":["2023YFF0713900"]}]},{"DOI":"10.13039\/501100004731","name":"Zhejiang Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["LTGC24B050009"],"award-info":[{"award-number":["LTGC24B050009"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"award":["LTGC24B050009"],"award-info":[{"award-number":["LTGC24B050009"]}],"id":[{"id":"https:\/\/ror.org\/04aqat463","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background\/Objectives: The whole plant of Tetrastigma hemsleyanum is used medicinally, but the tuberous root is by far the most commonly used part, and the way in which its metabolites are partitioned among organs and among geographic origins underpins both the rational choice of the medicinal part and the evaluation of herb quality. Methods: Here, untargeted metabolomics based on ultra-high-performance liquid chromatography coupled to high-resolution Orbitrap mass spectrometry was used to compare roots, stems and leaves collected from nine regions of southern China (81 samples) with tuberous roots collected from 17 sites in seven provinces (51 samples). Annotations were graded according to the Metabolomics Standards Initiative (MSI), curated with explicit plausibility rules, and every conclusion was re-tested across five nested annotation subsets. Results: Organ identity was the dominant source of metabolic variation: the three organs were completely separable (random forest out-of-bag accuracy 100%), the organ effect was about four times larger than that of sampling region (PERMANOVA pseudo-F 16.7 versus 4.1), and this contrast was essentially unchanged from the complete set of 615 annotations down to the most stringent subset of 19 flavonoids and phenolic acids. The organ-level pattern was chemically coherent: amino acids and lipids were relatively enriched in the tuberous root, soluble sugars and phenolic acids in the stem, and flavonoids and alkaloids in the leaf, matching the contrasting roles of a storage, a transport and a photosynthetic organ. Geographic differences among tuberous roots were, by contrast, weak and largely local: although provinces could be separated with 88.9% out-of-bag accuracy, accuracy fell to 42.2% when an entire, previously unseen collection site was held out (chance level 20%), and to 52.9% for a coarse macro-geographic zone (chance level 25%), whereas holding out an entire sampling region left organ classification unaffected (100%). Conclusions: The present data therefore document a strong, generalisable and chemically interpretable organ division of labour, but do not support the use of this metabolome for origin authentication. Because most annotations remain at MSI Level 3, individual compounds are reported as putative throughout, and all conclusions rest on multivariate and class-level evidence.<\/jats:p>","DOI":"10.3390\/metabo16090619","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:47:13Z","timestamp":1787849233000},"page":"619","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Organ Identity Outweighs Geographic Origin in Shaping the Metabolome of Tetrastigma hemsleyanum"],"prefix":"10.3390","volume":"16","author":[{"given":"Mingli","family":"Ye","sequence":"first","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukai","family":"Ba","sequence":"additional","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8416-2711","authenticated-orcid":false,"given":"Zhengrui","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boya","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9213-3000","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoya","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7813-8419","authenticated-orcid":false,"given":"Yonggang","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ban","family":"Cao","sequence":"additional","affiliation":[{"name":"Zhejiang Institute of Geosciences, Hangzhou 310000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113247","DOI":"10.1016\/j.jep.2020.113247","article-title":"A comprehensive review on traditional uses, chemical compositions, pharmacology properties and toxicology of Tetrastigma hemsleyanum","volume":"264","author":"Ji","year":"2021","journal-title":"J. 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