{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:16:18Z","timestamp":1788434178333,"version":"build-2803163510"},"reference-count":105,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR24993004"],"award-info":[{"award-number":["BR24993004"]}]},{"award":["BR24993004"],"award-info":[{"award-number":["BR24993004"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Backgrounds: The present study investigated temperature-associated responses of microclones of two halophytic shrubs, Nitraria sibirica Pall. and Nitraria schoberi L., exposed to low positive (+3 \u00b0C) and elevated (+30 \u00b0C) temperatures for 72 h. Methods: Temperature-associated responses were investigated by evaluation of leaf anatomy, chlorophyll fluorescence, and GC\u2013MS-based metabolite profiling. Results: The results revealed shared and species-specific responses, including changes in leaf anatomical dimensions, maintenance or adjustment of PSII-related fluorescence parameters, and treatment-dependent shifts in the relative composition and diversity of putatively annotated metabolite classes. Metabolomic profiling was performed in both in vitro microclones and soil-acclimatised microclones. In vitro microclones showed more pronounced shifts in the number and relative representation of detected compound classes under temperature exposure, whereas soil-acclimatised microclones demonstrated comparatively greater compositional stability. Comparative analysis of the datasets revealed temperature-associated patterns across anatomical, photosynthetic, and relative metabolomic responses; however, these patterns were interpreted descriptively rather than as direct causal mechanisms. Some detected compounds or compound classes have been associated with biological relevance in previous studies; however, their identity, abundance, and activity require targeted validation. Conclusions: Overall, the findings provide a descriptive experimental dataset on temperature-associated anatomical, chlorophyll fluorescence, and relative GC\u2013MS profile changes in Nitraria microclones and identify candidate metabolite classes for future targeted validation.<\/jats:p>","DOI":"10.3390\/metabo16090646","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:10:33Z","timestamp":1788430233000},"page":"646","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Temperature-Associated Anatomical, Photosynthetic, and Putative GC\u2013MS Metabolomic Profiles in the Arid Species Nitraria sibirica Pall. and Nitraria schoberi L."],"prefix":"10.3390","volume":"16","author":[{"given":"Dariga","family":"Dairbekova","sequence":"first","affiliation":[{"name":"Faculty of Biology and Biotechnology, Farabi University, Al-Farabi 71\/19, Almaty 050040, Kazakhstan"},{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3176-820X","authenticated-orcid":false,"given":"Nina V.","family":"Terletskaya","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Farabi University, Al-Farabi 71\/19, Almaty 050040, Kazakhstan"},{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4274-5081","authenticated-orcid":false,"given":"Aigerim","family":"Mamirova","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Farabi University, Al-Farabi 71\/19, Almaty 050040, Kazakhstan"},{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0440-9180","authenticated-orcid":false,"given":"Nazym K.","family":"Korbozova","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Farabi University, Al-Farabi 71\/19, Almaty 050040, Kazakhstan"},{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7844-4264","authenticated-orcid":false,"given":"Elvira A.","family":"Shadenova","sequence":"additional","affiliation":[{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2871-1552","authenticated-orcid":false,"given":"Aizhan S.","family":"Mussayeva","sequence":"additional","affiliation":[{"name":"Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lovejoy, T.E., and Hannah, L. 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