{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:07:20Z","timestamp":1792789640810,"version":"4.5.0"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hechi University high-level talent research start-up fee project","award":["2021GCC017"],"award-info":[{"award-number":["2021GCC017"]}],"id":[{"id":"https:\/\/ror.org\/05pjkyk24","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Hechi University high-level talent research start-up fee project","award":["2021GCC023"],"award-info":[{"award-number":["2021GCC023"]}],"id":[{"id":"https:\/\/ror.org\/05pjkyk24","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2021GCC017"],"award-info":[{"award-number":["2021GCC017"]}],"id":[{"id":"https:\/\/ror.org\/05pjkyk24","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2021GCC023"],"award-info":[{"award-number":["2021GCC023"]}],"id":[{"id":"https:\/\/ror.org\/05pjkyk24","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Local Science and Technology Development Fund project guided by the central government","award":["Heke ZY230301"],"award-info":[{"award-number":["Heke ZY230301"]}]},{"name":"2023 Annual Operation Subsidy Project of the Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology","award":["23-026-08"],"award-info":[{"award-number":["23-026-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Background: Cibotium barometz (L.) J. Sm. is a medicinal fern whose rhizome is the sole officinal part, while roots, stems, leaves and young shoots are discarded as agricultural residues. The chemical rationale for this restriction, and the value of the discarded organs, remain undefined. Materials and methods: Here, untargeted LC\u2013MS\/MS metabolomics (UHPLC\u2013Orbitrap) of methanol\/acetonitrile\/water extracts was applied to five organs of C. barometz (three pooled biological replicates per organ), annotating 2765 polar-to-moderately-polar metabolites. Results: Organ identity dominated the metabolic architecture of the sampled population: the first two principal components separated all five organs, with leaf and root as the two mutually opposed metabolic extremes and stem, young shoot and rhizome forming a contiguous module. Exploratory pairwise OPLS-DA combined with FDR-controlled univariate criteria yielded 193\u20131223 differentially accumulated metabolites per comparison, and tissue-specificity analysis assigned 2031 metabolites (73.45%) predominantly to a single organ. Discussion: Metabolite accumulation patterns along the phenylpropanoid\u2013flavonoid pathway was spatially partitioned, with flavan-3-ols and isoflavones accumulating in root, flavone\/flavonol glycosides in leaf and predominantly hydroxycinnamic acids in rhizome and young shoot. Of 22 previously reported or candidate quality control constituents, putatively annotated terpenoid lactones were detected almost exclusively in the rhizome, whereas several phenolics showed markedly higher relative abundance in root or leaf. Conclusions: These relative-abundance data indicate that the non-officinal organs are chemically complementary potential resources; absolute quantification, bioactivity and safety evaluation are prerequisites before any medicinal valorisation.<\/jats:p>","DOI":"10.3390\/metabo16090693","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:09:35Z","timestamp":1790075375000},"page":"693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Organ-Resolved Metabolomics of Cibotium barometz Reveals Rhizome-Specific Terpenoid Lactones and Phenolic-Rich Non-Officinal Organs"],"prefix":"10.3390","volume":"16","author":[{"given":"Guole","family":"Qin","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Sericulture Ecology and Intelligent Technology Application, Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Guangxi Colleges Universities Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources, School of Chemistry and Bioengineering, Hechi University, Hechi 546300, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Daizu","family":"Xie","sequence":"additional","affiliation":[{"name":"Hechi Forestry Research Institute, Hechi 547000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Changqin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Zhongnong Qiaosheng (Fangchenggang) Traditional Chinese Medicine Technology Co., Ltd., Fangchenggang 538037, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6866-0943","authenticated-orcid":false,"given":"Delong","family":"Guan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Sericulture Ecology and Intelligent Technology Application, Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Guangxi Colleges Universities Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources, School of Chemistry and Bioengineering, Hechi University, Hechi 546300, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5314-9405","authenticated-orcid":false,"given":"Jing","family":"Song","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Sericulture Ecology and Intelligent Technology Application, Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Guangxi Colleges Universities Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources, School of Chemistry and Bioengineering, Hechi University, Hechi 546300, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106843","DOI":"10.1016\/j.fitote.2025.106843","article-title":"Botany, traditional uses, phytochemistry, pharmacology, processing, and applications of Cibotium barometz (L.) J. Sm.: A review","volume":"186","author":"Xu","year":"2025","journal-title":"Fitoterapia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"117818","DOI":"10.1016\/j.jep.2024.117818","article-title":"The traditional utilization, biological activity and chemical composition of edible fern species","volume":"324","author":"Dvorakova","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","unstructured":"Chinese Pharmacopoeia Commission (2005). Pharmacopoeia of the People\u2019s Republic of China."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Jiang, R.H., Liang, S.Q., Wu, F., Tang, L.M., Qin, B., Chen, Y.Y., Huang, Y.H., Li, K.X., and Zhang, X.C. (2023). Phylogenomic analysis, cryptic species discovery, and DNA barcoding of the genus Cibotium in China based on plastome data. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1183653"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e71040","DOI":"10.1002\/ece3.71040","article-title":"Adaptive Management Based on the Habitat Change of Cibotium barometz Under Synergistic Impact of Climate and Land Use Change-A Case Study of Guangxi, China","volume":"15","author":"Feng","year":"2025","journal-title":"Ecol. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.13057\/biodiv\/d210415","article-title":"Assessing the conservation status of tree fern Cibotium arachnoideum (C. Chr.) Holttum","volume":"21","author":"Praptosuwiryo","year":"2020","journal-title":"Biodiversitas J. Biol. Divers."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1021\/np9004097","article-title":"Inhibitors of osteoclast formation from rhizomes of Cibotium barometz","volume":"72","author":"Cuong","year":"2009","journal-title":"J. Nat. Prod."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.jep.2011.07.017","article-title":"Anti-osteoporosis activity of Cibotium barometz extract on ovariectomy-induced bone loss in rats","volume":"137","author":"Zhao","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.phytochem.2017.02.023","article-title":"Hepatoprotective hemiterpene glycosides from the rhizome of Cibotium barometz (L.) J. Sm","volume":"138","author":"Xie","year":"2017","journal-title":"Phytochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.jep.2009.07.017","article-title":"The constituents of Cibotium barometz and their permeability in the human Caco-2 monolayer cell model","volume":"125","author":"Wu","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_11","first-page":"1826299","article-title":"Network Pharmacology-Based Strategy to Investigate the Mechanisms of Cibotium barometz in Treating Osteoarthritis","volume":"2022","author":"Chen","year":"2022","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112612","DOI":"10.1016\/j.indcrop.2020.112612","article-title":"Biological activities and phytochemical content of the rhizome hairs of Cibotium barometz (Cibotiaceae)","volume":"153","author":"Heng","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Wang, F.P., Chen, L.J., Zhou, S.R., Tang, M., Zhang, X., and Yan, Y.H. (2025). Integrated metabolomic and transcriptomic analyses reveal temporal dynamics of secondary metabolite accumulation in Cibotium barometz rhizome. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1702726"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.synbio.2023.06.005","article-title":"Functional characterization of triterpene synthases in Cibotium barometz","volume":"8","author":"Ji","year":"2023","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1126\/science.1217411","article-title":"The rise of chemodiversity in plants","volume":"336","author":"Weng","year":"2012","journal-title":"Science"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1146\/annurev-arplant-042110-103814","article-title":"Convergent evolution in plant specialized metabolism","volume":"62","author":"Pichersky","year":"2011","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/tpj.16214","article-title":"Cell-type-specific metabolism in plants","volume":"114","author":"Daloso","year":"2023","journal-title":"Plant J. Cell Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1093\/mp\/sst080","article-title":"A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: Application in the study of rice metabolomics","volume":"6","author":"Chen","year":"2013","journal-title":"Mol. Plant"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Yang, W., Liu, F., Wu, G., Liang, S., Bai, X., Liu, B., Zhang, B., Chen, H., and Yang, J. (2024). Widely Targeted Metabolomics Analysis of the Roots, Stems, Leaves, Flowers, and Fruits of Camellia luteoflora, a Species with an Extremely Small Population. Molecules, 29.","DOI":"10.3390\/molecules29194754"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e5545","DOI":"10.1002\/bmc.5545","article-title":"Widely targeted metabolomics analysis of different parts of kudzu","volume":"37","author":"Xiong","year":"2023","journal-title":"Biomed. Chromatogr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Gao, J., Xiong, K., Zhou, W., and Li, W. (2021). Extensive Metabolite Profiling in the Unexploited Organs of Black Tiger for Their Potential Valorization in the Pharmaceutical Industry. Life, 11.","DOI":"10.3390\/life11060544"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wu, Z., Wang, X., Chen, M., Hu, H., Cao, J., Chai, T., and Wang, H. (2019). A Study on Tissue-Specific Metabolite Variations in Polygonum cuspidatum by High-Resolution Mass Spectrometry-Based Metabolic Profiling. Molecules, 24.","DOI":"10.3390\/molecules24061058"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Su, Y., Qin, G., Zhou, C., Guan, D., and Song, J. (2026). Untargeted LC\u2013MS\/MS Metabolite Profiling of Wild, Field-Domesticated and Tissue-Cultured Rhizomes of the Endangered Medicinal Fern Cibotium barometz. Life, 16.","DOI":"10.3390\/life16091435"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/978-1-4939-6990-6_22","article-title":"Metabolomic Bioinformatic Analysis","volume":"Volume 1606","author":"Dailey","year":"2017","journal-title":"Methods in Molecular Biology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1021\/acs.jproteome.5b00354","article-title":"Analysis of the Human Adult Urinary Metabolome Variations with Age, Body Mass Index, and Gender by Implementing a Comprehensive Workflow for Univariate and OPLS Statistical Analyses","volume":"14","author":"Roux","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1093\/bioinformatics\/bti042","article-title":"Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification","volume":"21","author":"Yanai","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kanehisa, M. (2002). The KEGG database. Novartis Found. Symp., 247, 91\u2013101; 101\u2013103, 119\u2013128, 244\u2013252.","DOI":"10.1002\/0470857897.ch8"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"20230362","DOI":"10.1098\/rstb.2023.0362","article-title":"Biotic interactions, evolutionary forces and the pan-plant specialized metabolism","volume":"379","author":"Feussner","year":"2024","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e202401676","DOI":"10.1002\/cbdv.202401676","article-title":"Metabolomic Diversity and Defensive Phenolic Compounds in Cloud Forest Ferns","volume":"22","author":"Mehltreter","year":"2025","journal-title":"Chem. Biodivers."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.21105\/joss.02017","article-title":"ggalluvial: Layered Grammar for Alluvial Plots","volume":"5","author":"Brunson","year":"2020","journal-title":"J. Open Source Softw."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1093\/biomet\/38.3-4.330","article-title":"On the comparison of several mean values: An alternative approach","volume":"38","author":"Welch","year":"1951","journal-title":"Biometrika"},{"key":"ref_32","first-page":"113","article-title":"Pairwise multiple comparison procedures with unequal n\u2019s and\/or variances: A Monte Carlo study","volume":"1","author":"Games","year":"1976","journal-title":"J. Educ. Stat."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"116546","DOI":"10.1016\/j.jep.2023.116546","article-title":"Spatially resolved metabolomics combined with bioactivity analyses to evaluate the pharmacological properties of two Radix Puerariae species","volume":"313","author":"Guo","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1038\/s41467-017-02168-x","article-title":"Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance","volume":"8","author":"Peng","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1104\/pp.17.00842","article-title":"Flavan-3-ols Are an Effective Chemical Defense against Rust Infection","volume":"175","author":"Ullah","year":"2017","journal-title":"Plant Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1104\/pp.113.232389","article-title":"Flavan-3-ols in Norway spruce: Biosynthesis, accumulation, and function in response to attack by the bark bee-tle-associated fungus Ceratocystis polonica","volume":"164","author":"Hammerbacher","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"115732","DOI":"10.1016\/j.carbpol.2019.115732","article-title":"Two novel polysaccharides from rhizomes of Cibotium barometz promote bone formation via activating the BMP2\/SMAD1 signaling pathway in MC3T3-E1 cells","volume":"231","author":"Huang","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e34883","DOI":"10.1097\/MD.0000000000034883","article-title":"Network pharmacology analysis and clinical verification of Jishe Qushi capsules in rheumatoid arthritis treatment","volume":"102","author":"Li","year":"2023","journal-title":"Medicine"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Guo, Z., Duan, Y., Zhao, Z., Yang, D., and Xu, X. (2024). HPLC Fingerprint Analysis of Cibotii rhizoma from Different Regions and Identification of Common Peaks by LC-MS. Pharmaceuticals, 17.","DOI":"10.3390\/ph17030313"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11306-007-0082-2","article-title":"Proposed minimum reporting standards for chemical analysis Chemical Analysis Working Group (CAWG) Metabolomics Standards Initiative (MSI)","volume":"3","author":"Sumner","year":"2007","journal-title":"Metabolomics"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/9\/693\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:41:42Z","timestamp":1790077302000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/16\/9\/693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":40,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["metabo16090693"],"URL":"https:\/\/doi.org\/10.3390\/metabo16090693","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}