{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:27:29Z","timestamp":1760243249260,"version":"build-2065373602"},"reference-count":17,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T00:00:00Z","timestamp":1398124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Xanthomonas is a genus of phytopathogenic bacteria, which produces a slimy, polysaccharide matrix known as xanthan gum, which involves, protects and helps the bacteria during host colonization. Although broadly used as a stabilizer and thickener in the cosmetic and food industries, xanthan gum can be a troubling artifact in molecular investigations due to its rheological properties. In particular, a cross-reaction between reference compounds and the xanthan gum could compromise metabolic quantification by NMR spectroscopy. Aiming at an efficient gum extraction protocol, for a 1H-NMR-based metabolic profiling study of Xanthomonas, we tested four different interventions on the broadly used methanol-chloroform extraction protocol for the intracellular metabolic contents observation. Lower limits for bacterial pellet volumes for extraction were also probed, and a strategy is illustrated with an initial analysis of X. citri\u2019s metabolism by  1H-NMR spectroscopy.<\/jats:p>","DOI":"10.3390\/metabo4020218","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T12:18:37Z","timestamp":1398169117000},"page":"218-231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Xanthan Gum Removal for 1H-NMR Analysis of the Intracellular Metabolome of the Bacteria  Xanthomonas axonopodis pv. citri 306"],"prefix":"10.3390","volume":"4","author":[{"given":"Vanessa","family":"Pegos","sequence":"first","affiliation":[{"name":"Brazilian Biosciences National Laboratory, Rua Giuseppe M\u00e1ximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Canevarolo","sequence":"additional","affiliation":[{"name":"Brazilian Biosciences National Laboratory, Rua Giuseppe M\u00e1ximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas\/SP, Brazil"},{"name":"Molecular Biology Laboratory, Boldrini Children Hospital, Rua Dr. Gabriel Porto, 1270 Cidade Universit\u00e1ria, CEP 13083-210, Campinas\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Brazilian Biosciences National Laboratory, Rua Giuseppe M\u00e1ximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Balan","sequence":"additional","affiliation":[{"name":"Brazilian Biosciences National Laboratory, Rua Giuseppe M\u00e1ximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Zeri","sequence":"additional","affiliation":[{"name":"Brazilian Biosciences National Laboratory, Rua Giuseppe M\u00e1ximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nrmicro2558","article-title":"Pathogenomics of xanthomonas: Understanding bacterium-plant interactions","volume":"9","author":"Ryan","year":"2011","journal-title":"Nat. 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A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia Coli and Related Bacteria, Cold Spring Harbor Laboratory Press.","key":"ref_17"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/4\/2\/218\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:10:36Z","timestamp":1760217036000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/4\/2\/218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,22]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,6]]}},"alternative-id":["metabo4020218"],"URL":"https:\/\/doi.org\/10.3390\/metabo4020218","relation":{},"ISSN":["2218-1989"],"issn-type":[{"type":"electronic","value":"2218-1989"}],"subject":[],"published":{"date-parts":[[2014,4,22]]}}}