{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:59:09Z","timestamp":1791579549541,"version":"4.3.4"},"reference-count":25,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T00:00:00Z","timestamp":1434499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Lipidomic approaches are now widely used to investigate the relationship between lipid metabolism, health and disease. Large-scale lipidomics studies typically aim to quantify hundreds to thousands of lipid molecular species in a large number of samples. Consequently, high throughput methodology that can efficiently extract a wide range of lipids from biological samples is required. Current methods often rely on extraction in chloroform:methanol with or without two phase partitioning or other solvents, which are often incompatible with liquid chromatography electrospray ionization-tandem mass spectrometry (LC ESI-MS\/MS). Here, we present a fast, simple extraction method that is suitable for high throughput LC ESI-MS\/MS. Plasma (10 \u03bcL) was mixed with 100 \u03bcL  1-butanol:methanol (1:1 v\/v) containing internal standards resulting in efficient extraction of all major lipid classes (including sterols, glycerolipids, glycerophospholipids and sphingolipids). Lipids were quantified using positive-ion mode LC ESI-MS\/MS. The method showed high recovery (&gt;90%) and reproducibility (%CV &lt; 20%). It showed a strong correlation of all lipid measures with an established chloroform:methanol extraction method (R2 = 0.976). This method uses non-halogenated solvents, requires no drying or reconstitution steps and is suitable for large-scale LC ESI-MS\/MS-based lipidomic analyses in research and clinical laboratories.<\/jats:p>","DOI":"10.3390\/metabo5020389","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T11:19:34Z","timestamp":1434539974000},"page":"389-403","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":206,"title":["An Efficient Single Phase Method for the Extraction of  Plasma Lipids"],"prefix":"10.3390","volume":"5","author":[{"given":"Zahir","family":"Alshehry","sequence":"first","affiliation":[{"name":"Baker IDI, Heart and Diabetes Institute, Melbourne, VIC 3004, Australia"},{"name":"King Fahad Medical City, Riyadh 11525, Saudi Arabia"},{"name":"The Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Melbourne, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Barlow","sequence":"additional","affiliation":[{"name":"Baker IDI, Heart and Diabetes Institute, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquelyn","family":"Weir","sequence":"additional","affiliation":[{"name":"Baker IDI, Heart and Diabetes Institute, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Baker IDI, Heart and Diabetes Institute, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm","family":"McConville","sequence":"additional","affiliation":[{"name":"The Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Melbourne, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Meikle","sequence":"additional","affiliation":[{"name":"Baker IDI, Heart and Diabetes Institute, Melbourne, VIC 3004, Australia"},{"name":"King Fahad Medical City, Riyadh 11525, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.amjcard.2007.07.037","article-title":"Relation of baseline plasma phospholipid levels to cardiovascular outcomes at two years in men with acute coronary syndrome referred for coronary angiography","volume":"100","author":"Cavusoglu","year":"2007","journal-title":"Am. J. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1161\/ATVBAHA.108.164723","article-title":"Transient increase in plasma oxidized ldl during the progression of atherosclerosis in apolipoprotein e knockout mice","volume":"29","author":"Kato","year":"2009","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/S0140-6736(12)60312-2","article-title":"Plasma hdl cholesterol and risk of myocardial infarction: A mendelian randomisation study","volume":"380","author":"Voight","year":"2012","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1007\/s11745-010-3453-9","article-title":"Increased lipid peroxidation in ldl from type-2 diabetic patients","volume":"45","author":"Colas","year":"2010","journal-title":"Lipids"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1242\/dmm.001180","article-title":"A comprehensive definition for metabolic syndrome","volume":"2","author":"Huang","year":"2009","journal-title":"Dis. Model. Mech."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1161\/ATVBAHA.111.234096","article-title":"Plasma lipidomic analysis of stable and unstable coronary artery disease","volume":"31","author":"Meikle","year":"2011","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1194\/jlr.P035808","article-title":"Plasma lipid profiling in a large population-based cohort","volume":"54","author":"Weir","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pharmthera.2014.02.001","article-title":"Lipidomics: Potential role in risk prediction and therapeutic monitoring for diabetes and cardiovascular disease","volume":"143","author":"Meikle","year":"2014","journal-title":"Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.2337\/db09-0206","article-title":"Apociii-enriched ldl in type 2 diabetes displays altered lipid composition, increased susceptibility for sphingomyelinase, and increased binding to biglycan","volume":"58","author":"Hiukka","year":"2009","journal-title":"Diabetes"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1039\/c1mb05342f","article-title":"Application of plasma lipidomics in studying the response of patients with essential hypertension to antihypertensive drug therapy","volume":"7","author":"Hu","year":"2011","journal-title":"Mol. Biosyst."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1016\/j.bbalip.2013.04.011","article-title":"Molecular lipidomics of exosomes released by pc-3 prostate cancer cells","volume":"1831","author":"Llorente","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.biocel.2014.01.026","article-title":"Localized lipidomics in cystic fibrosis: Tof-sims imaging of lungs from pseudomonas aeruginosa-infected mice","volume":"52","author":"Desbenoit","year":"2014","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e3863","DOI":"10.1371\/journal.pone.0003863","article-title":"Metabolic profiling reveals distinct variations linked to nicotine consumption in humans\u2013\u2013First results from the kora study","volume":"3","author":"Yu","year":"2008","journal-title":"PLoS ONE"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.1371\/journal.pone.0000097","article-title":"A systems biology strategy reveals biological pathways and plasma biomarker candidates for potentially toxic statin-induced changes in muscle","volume":"1","author":"Laaksonen","year":"2006","journal-title":"PLoS ONE"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s11883-010-0110-y","article-title":"Lipidomics: A tool for studies of atherosclerosis","volume":"12","author":"Ekroos","year":"2010","journal-title":"Curr. Atheroscler. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1038\/sj.mp.4002000","article-title":"Metabolomic mapping of atypical antipsychotic effects in schizophrenia","volume":"12","author":"McEvoy","year":"2007","journal-title":"Mol. Psychiatry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1097\/MOL.0b013e3283453dbe","article-title":"Lipidomics is providing new insight into the metabolic syndrome and its sequelae","volume":"22","author":"Meikle","year":"2011","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.1016\/j.jchromb.2009.02.037","article-title":"High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry","volume":"877","author":"Stahlman","year":"2009","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0009-3084(91)90018-7","article-title":"An improved synthesis of 7-nitrobenz-2-oxa-1,3-diazole analogs of cdp-diacylglycerol and phosphatidylinositol","volume":"60","author":"Ting","year":"1991","journal-title":"Chem. Phys. Lipids"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1194\/jlr.D023036","article-title":"The bume method: A novel automated chloroform-free 96-well total lipid extraction method for blood plasma","volume":"53","author":"Lofgren","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1194\/jlr.M034330","article-title":"A comparison of five lipid extraction solvent systems for lipidomic studies of human ldl","volume":"54","author":"Reis","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1194\/jlr.D050302","article-title":"A monophasic extraction strategy for the simultaneous lipidome analysis of polar and nonpolar retina lipids","volume":"55","author":"Lydic","year":"2014","journal-title":"J. Lipid Res."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/5\/2\/389\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:48:03Z","timestamp":1760215683000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/5\/2\/389"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,17]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,6]]}},"alternative-id":["metabo5020389"],"URL":"https:\/\/doi.org\/10.3390\/metabo5020389","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,17]]}}}