{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:19:47Z","timestamp":1774484387398,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T00:00:00Z","timestamp":1482278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL126711"],"award-info":[{"award-number":["HL126711"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Pittsburgh Physicians Foundation Award","award":["4-29433"],"award-info":[{"award-number":["4-29433"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide important insights to the pathophysiology of sepsis recovery. In this study, we explored metabolomics in a novel Drosophila melanogaster model of surviving sepsis using Nuclear Magnetic Resonance (NMR), to determine metabolite profiles. We used a model of percutaneous infection in Drosophila melanogaster to mimic sepsis. We had three experimental groups: sepsis survivors (infected with Staphylococcus aureus and treated with oral linezolid), sham (pricked with an aseptic needle), and unmanipulated (positive control). We performed metabolic measurements seven days after sepsis. We then implemented metabolites detected in NMR spectra into the MetExplore web server in order to identify the metabolic pathway alterations in sepsis surviving Drosophila. Our NMR metabolomic approach in a Drosophila model of recovery from sepsis clearly distinguished between all three groups and showed two different metabolomic signatures of inflammation. Sham flies had decreased levels of maltose, alanine, and glutamine, while their level of choline was increased. Sepsis survivors had a metabolic signature characterized by decreased glucose, maltose, tyrosine, beta-alanine, acetate, glutamine, and succinate.<\/jats:p>","DOI":"10.3390\/metabo6040047","type":"journal-article","created":{"date-parts":[[2016,12,23]],"date-time":"2016-12-23T04:09:09Z","timestamp":1482466149000},"page":"47","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis"],"prefix":"10.3390","volume":"6","author":[{"given":"Veli","family":"Bakalov","sequence":"first","affiliation":[{"name":"Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0024-6134","authenticated-orcid":false,"given":"Roland","family":"Amathieu","sequence":"additional","affiliation":[{"name":"Laboratoire Chimie, Structures, Propri\u00e9t\u00e9s de Biomat\u00e9riaux et d\u2019Agents Th\u00e9rapeutiques (CSPBAT), UMR 7244, Universit\u00e9 Paris 13, 93000 Bobigny, France"},{"name":"Intensive Care Unit, Jean Verdier University Hospital, Paris 13 University, 93140 Bondy, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Triba","sequence":"additional","affiliation":[{"name":"Laboratoire Chimie, Structures, Propri\u00e9t\u00e9s de Biomat\u00e9riaux et d\u2019Agents Th\u00e9rapeutiques (CSPBAT), UMR 7244, Universit\u00e9 Paris 13, 93000 Bobigny, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Jeanne","family":"Cl\u00e9ment","sequence":"additional","affiliation":[{"name":"Laboratoire Structure\u2014Activit\u00e9 des biomol\u00e9cules Normales et Pathologiques (SABNP), U1204, Universit\u00e9 d\u2019Evry Val d\u2019Essonne, 91025 Evry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Reyes Uribe","sequence":"additional","affiliation":[{"name":"Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6791-7935","authenticated-orcid":false,"given":"Laurence","family":"Le Moyec","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie Int\u00e9grative des Adaptations \u00e0 l\u2019Exercice (UBIAE), EA 7362, Universit\u00e9 d\u2019Evry Val d\u2019Essonne, 91025 Evry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ata","family":"Kaynar","sequence":"additional","affiliation":[{"name":"Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA"},{"name":"Department of Anesthesiology, University of Pittsburgh, Pittsburgh, 15261 PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yende, S., D\u2019Angelo, G., Mayr, F., Kellum, J.A., Weissfeld, L., Kaynar, A.M., Young, T., Irani, K., and Angus, D.C. 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