{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:16:56Z","timestamp":1788535016072,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Bloodstream infection with Candida fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to Candida albicans, the rising incidence of infections caused by other Candida strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of Candida. To this end, here we present the characterization of the dielectrophoresis (DEP) response of Candida albicans, Candida tropicalis and Candida parapsilosis. We complement such characterization with a study of the Candida cells morphology. The Candida strains exhibited subtle differences in their morphology and dimensions. All the Candida strains exhibited positive DEP in the range 10\u2013500 kHz, although the strength of the DEP response was different for each Candida strain at different frequencies. Only Candida tropicalis showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific Candida strain at specific DEP conditions towards aiding in the rapid identification of Candida strains to enable the effective and timely treatment of Candida infections.<\/jats:p>","DOI":"10.3390\/mi11030255","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T06:34:23Z","timestamp":1583130863000},"page":"255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4847-3996","authenticated-orcid":false,"given":"Monsur","family":"Islam","sequence":"first","affiliation":[{"name":"Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USA"},{"name":"Institute for Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devin","family":"Keck","sequence":"additional","affiliation":[{"name":"Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordon","family":"Gilmore","sequence":"additional","affiliation":[{"name":"Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USA"},{"name":"Department of Bioengineering, 301 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8023-1161","authenticated-orcid":false,"given":"Rodrigo","family":"Martinez-Duarte","sequence":"additional","affiliation":[{"name":"Multiscale Manufacturing Laboratory, Mechanical Engineering Department, Clemson University, Clemson, SC 29634, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.7883\/yoken.JJID.2005.344","article-title":"Emergence of non-albicans Candida species and antifungal resistance in a tertiary care hospital","volume":"58","author":"Capoor","year":"2005","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.4103\/2394-2010.208115","article-title":"Increased role of nonalbicans Candida, potential risk factors, and attributable mortality in hospitalized patients","volume":"4","author":"Sandhu","year":"2017","journal-title":"J. Health Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3522","DOI":"10.1128\/JCM.00403-07","article-title":"Multicenter comparison of the VITEK 2 antifungal susceptibility test with the CLSI broth microdilution reference method for testing amphotericin B, flucytosine, and voriconazole against Candida spp.","volume":"45","author":"Pfaller","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3923\/mj.2011.1.7","article-title":"A comprehensive review of the occurance and management of systematic candidiasis as an opportunistic infection","volume":"1","author":"Chakravarthi","year":"2011","journal-title":"Microbiol. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.ijantimicag.2006.01.002","article-title":"Candidaemia in Europe: Epidemiology and resistance","volume":"27","author":"Tortorano","year":"2006","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1099\/jmm.0.045054-0","article-title":"Candida species: Current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options","volume":"62","author":"Sardi","year":"2013","journal-title":"J. Med. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"839","DOI":"10.3109\/13693780903580121","article-title":"Five-year evaluation of bloodstream yeast infections in a tertiary hospital: The predominance of non-C. albicans Candida species","volume":"48","author":"Pereira","year":"2010","journal-title":"Med. Mycol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Islam, M., Natu, R., Larraga-Martinez, M.F., and Martinez-Duarte, R. (2016). Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis. Biomicrofluidics, 10.","DOI":"10.1063\/1.4954310"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yildizhan, Y., Erdem, N., Islam, M., Martinez-Duarte, R., and Elitas, M. (2017). Dielectrophoretic separation of live and dead monocytes using 3D carbon-electrodes. Sensors, 17.","DOI":"10.3390\/s17112691"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.1002\/elps.201600551","article-title":"Assessing the importance of the root mean square (RMS) value of different waveforms to determine the strength of a dielectrophoresis trapping force","volume":"38","author":"Gilmore","year":"2017","journal-title":"Electrophoresis"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Natu, R., Islam, M., and Martinez-duarte, R. (2019). Nondimensional Streaming Dielectrophoresis Number for a System of Continuous Particle Separation. Anal. Chem.","DOI":"10.1021\/acs.analchem.8b04599"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Elitas, M., Yildizhan, Y., Islam, M., Martinez-Duarte, R., and Ozkazanc, D. (2018). Dielectrophoretic characterization and separation of monocytes and macrophages using 3D carbon-electrodes. Electrophoresis.","DOI":"10.1002\/elps.201800324"},{"key":"ref_13","unstructured":"Martinez-Duarte, R. (2010). Label-Free Cell Sorting Using Carbon-Electrode Dielectrophoresis and Centrifugal Microfluidics, University of California."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.anchem.111808.073610","article-title":"Microsystems for the capture of low-abundance cells","volume":"3","author":"Dharmasiri","year":"2010","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1002\/elps.200800373","article-title":"Isolation of rare cells from cell mixtures by dielectrophoresis","volume":"30","author":"Gascoyne","year":"2009","journal-title":"Electrophoresis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.mimet.2005.04.027","article-title":"An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water","volume":"62","author":"Davalos","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1039\/b110990c","article-title":"Microsample preparation by dielectrophoresis: Isolation of malaria","volume":"2","author":"Gascoyne","year":"2002","journal-title":"Lab Chip"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1016\/S0006-3495(02)73977-5","article-title":"Electrodeless dielectrophoresis of single- and double-stranded DNA","volume":"83","author":"Chou","year":"2002","journal-title":"Biophys. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3725","DOI":"10.1002\/elps.200500129","article-title":"Aligning fast alternating current electroosmotic flow fields and characteristic frequencies with dielectrophoretic traps to achieve rapid bacteria detection","volume":"26","author":"Gagnon","year":"2005","journal-title":"Electrophoresis"},{"key":"ref_20","first-page":"18","article-title":"Trapping single molecules by dielectrophoresis","volume":"95","author":"Calander","year":"2005","journal-title":"Phys. Rev. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1063\/1.2723669","article-title":"An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting","volume":"1","author":"Cheng","year":"2007","journal-title":"Biomicrofluidics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3917","DOI":"10.1039\/C4LC00669K","article-title":"An integrated microfluidic device utilizing dielectrophoresis and multiplex array PCR for point-of-care detection of pathogens","volume":"14","author":"Cai","year":"2014","journal-title":"Lab Chip"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bios.2011.12.005","article-title":"Dielectrophoresis and shear-enhanced sensitivity and selectivity of DNA hybridization for the rapid discrimination of Candida species","volume":"33","author":"Cheng","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1002\/elps.201100107","article-title":"Dielectrophoretic monitoring of microorganisms in environmental applications","volume":"32","year":"2011","journal-title":"Electrophoresis"},{"key":"ref_25","unstructured":"Cheng, I.-F., Chang, H.-C., and Chung, C.C. (2014). Microbial Identification and Manipulation of Nanoscale Biomolecules. (U.S. Patent 8808518B2)."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1039\/c2lc41128h","article-title":"Micro-organism extraction from biological samples using DEP forces enhanced by osmotic shock","volume":"13","author":"Bisceglia","year":"2013","journal-title":"Lab Chip"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s10404-011-0852-z","article-title":"Stepwise gray-scale light-induced electric field gradient for passive and continuous separation of microparticles","volume":"12","author":"Cheng","year":"2012","journal-title":"Microfluid. Nanofluid."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Low, W.S., and Wan Abas, W.A.B. (2015). Benchtop technologies for circulating tumor cells separation based on biophysical properties. Biomed Res. Int., 2015.","DOI":"10.1155\/2015\/239362"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1002\/elps.200800614","article-title":"Bioanalytical separations using electric field gradient techniques","volume":"30","author":"Meighan","year":"2009","journal-title":"Electrophoresis"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Natu, R., and Martinez-Duarte, R. (2016). Numerical Model of Streaming DEP for Stem Cell Sorting. Micromachines, 7.","DOI":"10.3390\/mi7120217"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.bios.2012.12.049","article-title":"Increasing PCR sensitivity by removal of polymerase inhibitors in environmental samples by using dielectrophoresis","volume":"43","author":"Jaramillo","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1002\/elps.201100059","article-title":"A novel approach to dielectrophoresis using carbon electrodes","volume":"32","author":"Renaud","year":"2011","journal-title":"Electrophoresis"},{"key":"ref_33","first-page":"25","article-title":"Fluido-Dynamic and Electromagnetic Characterization of 3D Carbon Dielectrophoresis with Finite Element Analysis","volume":"3","author":"Cito","year":"2008","journal-title":"Sens. Transducers J."},{"key":"ref_34","unstructured":"Madou, M.J., Perez-Gonzalez, V.H., and Pramanick, B. (2015). Carbon MEMS for Selected Lab-on-a-Chip Applications. Carbon: The Next Silicon? Book-2 Applications, Momentum Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1039\/b925456k","article-title":"The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform","volume":"10","author":"Gorkin","year":"2010","journal-title":"Lab Chip"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1002\/elps.201200447","article-title":"Dielectrophoresis of lambda-DNA using 3D carbon electrodes","volume":"34","author":"Renaud","year":"2013","journal-title":"Electrophoresis"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1039\/C4LC00109E","article-title":"Dielectrophoresis-based purification of antibiotic-treated bacterial subpopulations","volume":"14","author":"Elitas","year":"2014","journal-title":"Lab Chip"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Islam, M., Natu, R., and Martinez-Duarte, R. (2015). A study on the limits and advantages of using a desktop cutter plotter to fabricate microfluidic networks. Microfluid. Nanofluid.","DOI":"10.1007\/s10404-015-1626-9"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/j.1574-6976.2011.00278.x","article-title":"Candida glabrata, Candida parapsilosis and Candida tropicalis: Biology, epidemiology, pathogenicity and antifungal resistance","volume":"36","author":"Silva","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1590\/S1517-83822013005000056","article-title":"Candida albicans morphologies revealed by scanning electron microscopy analysis","volume":"44","author":"Staniszewska","year":"2013","journal-title":"Braz. J. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1128\/jcm.35.1.313-316.1997","article-title":"Candida and Torulopsis: A blinded evaluation of use of pseudohypha formation as basis for identification of medically important yeasts","volume":"35","author":"Odds","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1099\/00221287-144-2-403","article-title":"Depolarized cell growth precedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis","volume":"144","author":"Suzuki","year":"1998","journal-title":"Microbiology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/j.1462-5822.2005.00659.x","article-title":"Neutrophil extracellular traps capture and kill Candida albicans and hyphal forms","volume":"8","author":"Urban","year":"2006","journal-title":"Cell. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Desai, J.V. (2018). Candida albicans hyphae: From growth initiation to invasion. J. Fungi, 4.","DOI":"10.3390\/jof4010010"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1002\/pmic.200300608","article-title":"Two-dimensional reference map of Candida albicans hyphal forms","volume":"4","author":"Nombela","year":"2004","journal-title":"Proteomics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1063\/1.868626","article-title":"Stokes drag on conglomerates of spheres","volume":"7","author":"Cichocki","year":"1995","journal-title":"Phys. Fluids"},{"key":"ref_47","unstructured":"Martinez-Duarte, R., Andrade-Roman, J., Martinez, S.O., and Madou, M.J. (2008, January 1\u20135). A High Throughput Multi-stage, Multi-frequency Filter and Separation Device based on Carbon Dielectrophoresis. Proceedings of the 11th Annual NSTI Nanotechnology Conference and Trade Show, Boston, MA, USA."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e0187509","DOI":"10.1371\/journal.pone.0187509","article-title":"Theoretical prediction and validation of cell recovery rates in preparing platelet-rich plasma through a centrifugation","volume":"12","author":"Piao","year":"2017","journal-title":"PLoS ONE"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-39175-5","article-title":"Viscoelastic Separation and Concentration of Fungi from Blood for Highly Sensitive Molecular Diagnostics","volume":"9","author":"Nam","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"182581","DOI":"10.1155\/2010\/182581","article-title":"Dielectrophoresis: A review of applications for stem cell research","volume":"2010","author":"Pethig","year":"2010","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_51","first-page":"913","article-title":"Separation of white blood cells from erythrocytes on a dielectrophoresis (DEP) based \u201cLab-on-a-chip\u201d device","volume":"15","author":"Borgatti","year":"2005","journal-title":"Int. J. Mol. Med."}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/11\/3\/255\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:02:32Z","timestamp":1760173352000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/11\/3\/255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,28]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["mi11030255"],"URL":"https:\/\/doi.org\/10.3390\/mi11030255","relation":{},"ISSN":["2072-666X"],"issn-type":[{"value":"2072-666X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,28]]}}}