{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T22:55:56Z","timestamp":1778194556000,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T00:00:00Z","timestamp":1620345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["62073208, 61933008, 61803250"],"award-info":[{"award-number":["62073208, 61933008, 61803250"]}]},{"name":"Joint Fund of Science and Technology Department of Liaoning Province and State Key Laboratory of Robotics","award":["Grant 2020-KF-22-07"],"award-info":[{"award-number":["Grant 2020-KF-22-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>The identification and treatment of androgen-independent prostate cancer are both challenging and significant. In this work, high-throughput deformability cytometry was employed to assess the effects of two anti-cancer drugs, docetaxel and enzalutamide, on androgen-sensitive prostate cancer cells (LNCaP) and androgen-independent prostate cancer cells (PC-3), respectively. The quantified results show that PC-3 and LNCaP present not only different intrinsic physical properties but also different physical responses to the same anti-cancer drug. PC-3 cells possess greater stiffness and a smaller size than LNCaP cells. As the docetaxel concentration increases, PC-3 cells present an increase in stiffness and size, but LNCaP cells only present an increase in stiffness. As the enzalutamide concentration increases, PC-3 cells present no physical changes but LNCaP cells present changes in both cell size and deformation. These results demonstrated that cellular physical properties quantified by the deformability cytometry are effective indicators for identifying the androgen-independent prostate cancer cells from androgen-sensitive prostate cancer cells and evaluating drug effects on these two types of prostate cancer.<\/jats:p>","DOI":"10.3390\/mi12050532","type":"journal-article","created":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T10:06:12Z","timestamp":1620381972000},"page":"532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Microfluidic Assessment of Drug Effects on Physical Properties of Androgen Sensitive and Non-Sensitive Prostate Cancer Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Da","family":"Luo","sequence":"first","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1312-9527","authenticated-orcid":false,"given":"Yan","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8321-1898","authenticated-orcid":false,"given":"Tao","family":"Yue","sequence":"additional","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Cao","sequence":"additional","affiliation":[{"name":"Material Genome Institute, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1016\/S0140-6736(08)60729-1","article-title":"Prostate cancer","volume":"371","author":"Damber","year":"2008","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3251","DOI":"10.1158\/1078-0432.CCR-08-1171","article-title":"Castration-resistant prostate cancer: Locking up the molecular escape routes","volume":"15","author":"Attar","year":"2009","journal-title":"Clin. Cancer Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"901","DOI":"10.4161\/cbt.5.8.2949","article-title":"Stem cells in prostate cancer: Resolving the castrate-resistant conundrum and implications for hormonal therapy","volume":"5","author":"Sharifi","year":"2006","journal-title":"Cancer Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1158\/0008-5472.CAN-11-3004","article-title":"Androgen deprivation causes epithelial-mesenchymal transition in the prostate: Implications for androgen-deprivation therapy","volume":"72","author":"Sun","year":"2012","journal-title":"Cancer Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1146\/annurev-bioeng-071114-040545","article-title":"High-Throughput Assessment of Cellular Mechanical Properties","volume":"17","author":"Darling","year":"2015","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/978-1-4939-7346-0_15","article-title":"Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells","volume":"1678","author":"Herbig","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"212ra163","DOI":"10.1126\/scitranslmed.3006559","article-title":"Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping","volume":"5","author":"Tse","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7580","DOI":"10.1073\/pnas.1218806110","article-title":"Characterizing deformability and surface friction of cancer cells","volume":"110","author":"Byun","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.3390\/mi6111457","article-title":"Constriction Channel Based Single-Cell Mechanical Property Characterization","volume":"6","author":"Xue","year":"2015","journal-title":"Micromachines"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1039\/b803355b","article-title":"Measurement of elastic properties of prostate cancer cells using AFM","volume":"133","author":"Faria","year":"2008","journal-title":"Analyst"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1529\/biophysj.104.045476","article-title":"Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence","volume":"88","author":"Guck","year":"2005","journal-title":"Biophys. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/S0006-3495(00)76614-8","article-title":"Drug-Induced Changes of Cytoskeletal Structure and Mechanics in Fibroblasts: An Atomic Force Microscopy Study","volume":"78","author":"Rotsch","year":"2000","journal-title":"Biophys. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1038\/s41598-018-38199-7","article-title":"Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation","volume":"9","author":"Raudenska","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1700705","DOI":"10.1002\/smll.201700705","article-title":"Inertial Microfluidic Cell Stretcher (iMCS): Fully Automated, High-Throughput, and Near Real-Time Cell Mechanotyping","volume":"13","author":"Deng","year":"2017","journal-title":"Small"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.abb.2011.12.013","article-title":"Cancer cell detection in tissue sections using AFM","volume":"518","author":"Lekka","year":"2012","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/S0304-8853(98)00564-2","article-title":"Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry","volume":"194","author":"Fabry","year":"1999","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"0408011","DOI":"10.1115\/1.4029936","article-title":"The Application of Micropipette Aspiration in Molecular Mechanics of Single Cells","volume":"5","author":"Lee","year":"2014","journal-title":"J. Nanotechnol. Eng. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4865","DOI":"10.1039\/C5AN00250H","article-title":"Use of solid-state nanopores for sensing co-translocational deformation of nano-liposomes","volume":"140","author":"Goyal","year":"2015","journal-title":"Analyst"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1088\/1361-6528\/aa5e66","article-title":"Rapid structural analysis of nanomaterials in aqueous solutions","volume":"28","author":"Ryuzaki","year":"2017","journal-title":"Nanotechnology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1038\/nature08012","article-title":"Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis","volume":"459","author":"Spencer","year":"2009","journal-title":"Nature"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1109\/JMEMS.2020.3026726","article-title":"A Microfluidic Device With Optically-Controlled Electrodes for On-Demand Electrical Impedance Measurement of Targeted Single Cells","volume":"29","author":"Liu","year":"2020","journal-title":"J. Microelectromech. Syst."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1109\/TASE.2019.2963044","article-title":"Automated Parallel Electrical Characterization of Cells Using Optically-Induced Dielectrophoresis","volume":"17","author":"Liu","year":"2020","journal-title":"IEEE Trans. Autom. Sci. Eng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1021\/acs.analchem.9b03939","article-title":"Mechanical Low-Pass Filtering of Cells for Detection of Circulating Tumor Cells in Whole Blood","volume":"92","author":"Suzuki","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7630","DOI":"10.1073\/pnas.1200107109","article-title":"Hydrodynamic stretching of single cells for large population mechanical phenotyping","volume":"109","author":"Gossett","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/nmeth.3281","article-title":"Real-time deformability cytometry: On-the-fly cell mechanical phenotyping","volume":"12","author":"Otto","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2306","DOI":"10.1039\/D0LC00258E","article-title":"Label-free on chip quality assessment of cellular blood products using real-time deformability cytometry","volume":"20","author":"Aurich","year":"2020","journal-title":"Lab Chip"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41467-020-15813-9","article-title":"High-throughput cell and spheroid mechanics in virtual fluidic channels","volume":"11","author":"Panhwar","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Liu, N., Du, P., Xiao, X., Liu, Y., Peng, Y., Yang, C., and Yue, T. (2019). Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines. Micromachines, 10.","DOI":"10.3390\/mi10090602"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.bios.2019.111338","article-title":"Cell-line characterization by infrared-induced pyroelectric effect","volume":"140","author":"Pullano","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1021\/acsbiomaterials.6b00558","article-title":"Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties","volume":"3","author":"Mokbel","year":"2017","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1016\/j.bbagen.2016.06.010","article-title":"Cell stiffness determined by atomic force microscopy and its correlation with cell motility","volume":"1860","author":"Luo","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"43764","DOI":"10.1039\/C7RA06233H","article-title":"Interrogation of drug effects on HeLa cells by exploiting new AFM mechanical biomarkers","volume":"7","author":"Yun","year":"2017","journal-title":"RSC Adv."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.bpj.2019.01.034","article-title":"Cells Under Stress: An Inertial-Shear Microfluidic Determination of Cell Behavior","volume":"116","author":"Armistead","year":"2019","journal-title":"Biophys. J."}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/12\/5\/532\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:58:03Z","timestamp":1760162283000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/12\/5\/532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,7]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["mi12050532"],"URL":"https:\/\/doi.org\/10.3390\/mi12050532","relation":{},"ISSN":["2072-666X"],"issn-type":[{"value":"2072-666X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,7]]}}}