{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T13:30:45Z","timestamp":1774877445826,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T00:00:00Z","timestamp":1629417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"chungnam national sejong hospital","award":["2021"],"award-info":[{"award-number":["2021"]}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1A2C4001856"],"award-info":[{"award-number":["NRF-2020R1A2C4001856"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HR20C0025"],"award-info":[{"award-number":["HR20C0025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Background: Circulating tumor DNA (ctDNA) and exosome have been widely researched in the field of medical technology and diagnosis platforms. The purpose of our study was to improve the capturing properties of ctDNA and exosome, which involved combining two beads using approaches that may provide a new method for cancer diagnoses. Methods: We present a dual isolation system including a polydopamine (PDA)\u2013silica-coated alginate bead for circulating tumor DNA (ctDNA) capture and an anti-CD63 immobilized bead for exosome capture. We examined the ctDNA mutation in pre-operative plasma samples obtained from 91 colorectal cancer (CRC) patients using a droplet digital PCR (ddPCR). Results: The area under the curve (AUROC) of ctKRAS G12D mutation in the buffy coat was 0.718 (95% CI: 0.598\u22120.838; p = 0.001). Patients with CRC that had unmethylation of MLH1 and MSH2 showed significantly higher buffy coat ctKRAS G12D mutations, ascites ctKRAS G12D mutations, miR-31-5, and mixed scores than the patients with a methylation of MLH1 and MSH2. Conclusion: Our proposed alginate bead using the specific gravity-free method suggests that the screening of mutated ctKRAS DNA and miR-31-5 by liquid biopsy aids in identifying the patients, predicting a primary tumor, and monitoring in the early detection of a tumor.<\/jats:p>","DOI":"10.3390\/mi12080987","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T08:44:45Z","timestamp":1629449085000},"page":"987","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Specific Gravity-Free Method for the Isolation of Circulating Tumor KRAS Mutant DNA and Exosome in Colorectal Cancer"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1975-2961","authenticated-orcid":false,"given":"Tae Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Research Institute for Future Medical Science, Chungnam National University Sejong Hospital (CNUSH), Sejong 30099, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunsook","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro, 291, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-gon","family":"Goh","sequence":"additional","affiliation":[{"name":"Research Institute for Future Medical Science, Chungnam National University Sejong Hospital (CNUSH), Sejong 30099, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han Byul","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Institute for Future Medical Science, Chungnam National University Sejong Hospital (CNUSH), Sejong 30099, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1619-2660","authenticated-orcid":false,"given":"Woo Sun","family":"Rou","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chungnam National University Sejong Hospital, Sejong 30099, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0485-0072","authenticated-orcid":false,"given":"Hyuk Soo","family":"Eun","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chungnam National University Hospital, 282, Munwha-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Internal Medicine, College of Medicine, Chungnam National University, 266, Mun-wha-ro, Jung-gu, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1055\/s-2005-916277","article-title":"Preoperative evaluation and oncologic principles of colon cancer surgery","volume":"18","author":"Lynch","year":"2005","journal-title":"Clin. 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