{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:20:54Z","timestamp":1790418054803,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Foundation","award":["1849206"],"award-info":[{"award-number":["1849206"]}]},{"name":"National Science Foundation","award":["1736255"],"award-info":[{"award-number":["1736255"]}]},{"name":"National Science Foundation","award":["1920954"],"award-info":[{"award-number":["1920954"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Bacteria are capable of producing a specific type of biopolymer, termed exopolysaccharides (EPSs). EPSs from thermophile Geobacillus sp. strain WSUCF1 specifically can be assembled using cost-effective lignocellulosic biomass as the primary carbon substrate in lieu of traditional sugars. 5-fluorouracil (5-FU) is an FDA-approved, versatile chemotherapeutic that has yielded high efficacy against colon, rectum, and breast cancers. The present study investigates the feasibility of a 5% 5-fluorouracil film using thermophilic exopolysaccharides as the foundation in conjunction with a simple self-forming method. The drug-loaded film formulation was seen to be highly effective against A375 human malignant melanoma at its current concentration with viability of A375 dropping to 12% after six hours of treatment. A drug release profile revealed a slight burst release before it settled into an extended and maintained release of 5-FU. These initial findings provide evidence for the versatility of thermophilic exopolysaccharides produced from lignocellulosic biomass to act as a chemotherapeutic-delivering device and expand the overall applications of extremophilic EPSs.<\/jats:p>","DOI":"10.3390\/mi14051092","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T01:36:48Z","timestamp":1684805808000},"page":"1092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["5-Fluorouracil-Encapsulated Films Using Exopolysaccharides from a Thermophilic Bacterium Geobacillus sp. WSUCF1 for Topical Drug Delivery"],"prefix":"10.3390","volume":"14","author":[{"given":"Joseph M.","family":"Laubach","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA"},{"name":"BuG ReMeDEE Consortium, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5493-252X","authenticated-orcid":false,"given":"Rajesh K.","family":"Sani","sequence":"additional","affiliation":[{"name":"BuG ReMeDEE Consortium, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA"},{"name":"Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1038\/s41598-018-36983-z","article-title":"Genome analysis of a thermophilic exopolysaccharide-producing bacterium-Geobacillus sp. 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