{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T19:38:23Z","timestamp":1778701103123,"version":"3.51.4"},"reference-count":80,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Canada First Research Excellence Fund (CFREF)","award":["RGPIN-2019-03999"],"award-info":[{"award-number":["RGPIN-2019-03999"]}]},{"name":"Canada First Research Excellence Fund (CFREF)","award":["40161"],"award-info":[{"award-number":["40161"]}]},{"name":"Canadian Foundation for Innovation","award":["RGPIN-2019-03999"],"award-info":[{"award-number":["RGPIN-2019-03999"]}]},{"name":"Canadian Foundation for Innovation","award":["40161"],"award-info":[{"award-number":["40161"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>A continuous flow with reagent injection method on a novel inlaid microfluidic platform for nitrite determination has been successfully developed. The significance of the high-frequency monitoring of nutrient fluctuations in marine environments is crucial for understanding our impacts on the ecosystem. Many in-situ systems face limitations in high-frequency data collection and have restricted deployment times due to high reagent consumption. The proposed microfluidic device employs automatic colorimetric absorbance spectrophotometry, using the Griess assay for nitrite determination, with minimal reagent usage. The sensor incorporates 10 solenoid valves, four syringes, two LEDs, four photodiodes, and an inlaid microfluidic technique to facilitate optical measurements of fluid volumes. In this flow system, Taylor\u2013Aris dispersion was simulated for different injection volumes at a constant flow rate, and the results have been experimentally confirmed using red food dye injection into a carrier stream. A series of tests were conducted to determine a suitable injection frequency for the reagent. Following the initial system characterization, seven different standard concentrations ranging from 0.125 to 10 \u00b5M nitrite were run through the microfluidic device to acquire a calibration curve. Three different calibrations were performed to optimize plug length, with reagent injection volumes of 4, 20, and 50 \u00b5L. A straightforward signal processing method was implemented to mitigate the Schlieren effect caused by differences in refractive indexes between the reagent and standards. The results demonstrate that a sampling frequency of at least 10 samples per hour is achievable using this system. The obtained attenuation coefficients exhibited good agreement with the literature, while the reagent consumption was significantly reduced. The limit of detection for a 20 \u00b5L injection volume was determined to be 94 nM from the sample intake, and the limit of quantification was 312 nM. Going forward, the demonstrated system will be packaged in a submersible enclosure to facilitate in-situ colorimetric measurements in marine environments.<\/jats:p>","DOI":"10.3390\/mi15040519","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T09:28:06Z","timestamp":1712914086000},"page":"519","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Continuous Flow with Reagent Injection on an Inlaid Microfluidic Platform Applied to Nitrite Determination"],"prefix":"10.3390","volume":"15","author":[{"given":"Shahrooz","family":"Motahari","sequence":"first","affiliation":[{"name":"Department of Electrical & Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2449-7731","authenticated-orcid":false,"given":"Sean","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Oceanography, Dalhousie University, 1355 Oxford Street, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Hendricks","sequence":"additional","affiliation":[{"name":"Department of Electrical & Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Sonnichsen","sequence":"additional","affiliation":[{"name":"Department of Electrical & Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6289-9440","authenticated-orcid":false,"given":"Vincent","family":"Sieben","sequence":"additional","affiliation":[{"name":"Department of Electrical & Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1039\/c002672g","article-title":"Microfluidic colourimetric chemical analysis system: Application to nitrite detection","volume":"2","author":"Sieben","year":"2010","journal-title":"Anal. 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