{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:27:49Z","timestamp":1791494869931,"version":"4.3.4"},"reference-count":167,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T00:00:00Z","timestamp":1716768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62273289"],"award-info":[{"award-number":["62273289"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022KJ274"],"award-info":[{"award-number":["2022KJ274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Youth Innovation Science and Technology Support Program of Shandong Province","award":["62273289"],"award-info":[{"award-number":["62273289"]}]},{"name":"The Youth Innovation Science and Technology Support Program of Shandong Province","award":["2022KJ274"],"award-info":[{"award-number":["2022KJ274"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Circulating tumor cells (CTCs), derived from the primary tumor and carrying genetic information, contribute significantly to the process of tumor metastasis. The analysis and detection of CTCs can be used to assess the prognosis and treatment response in patients with tumors, as well as to help study the metastatic mechanisms of tumors and the development of new drugs. Since CTCs are very rare in the blood, it is a challenging problem to enrich CTCs efficiently. In this paper, we provide a comprehensive overview of microfluidics-based enrichment devices for CTCs in recent years. We explore in detail the methods of enrichment based on the physical or biological properties of CTCs; among them, physical properties cover factors such as size, density, and dielectric properties, while biological properties are mainly related to tumor-specific markers on the surface of CTCs. In addition, we provide an in-depth description of the methods for enrichment of single CTCs and illustrate the importance of single CTCs for performing tumor analyses. Future research will focus on aspects such as improving the separation efficiency, reducing costs, and increasing the detection sensitivity and accuracy.<\/jats:p>","DOI":"10.3390\/mi15060706","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T10:14:07Z","timestamp":1716804847000},"page":"706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review"],"prefix":"10.3390","volume":"15","author":[{"given":"Zezheng","family":"Qiao","sequence":"first","affiliation":[{"name":"School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Teng","sequence":"additional","affiliation":[{"name":"School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Mechanical and Electrical Engineering, Yantai Institute of Technology, Yantai 264005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1560-665X","authenticated-orcid":false,"given":"Wenguang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1038\/onc.2015.192","article-title":"The biology of circulating tumor cells","volume":"35","author":"Pantel","year":"2016","journal-title":"Oncogene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1039\/c2lc21012f","article-title":"Electrical fingerprinting, 3D profiling and detection of tumor cells with solid-state micropores","volume":"12","author":"Asghar","year":"2012","journal-title":"Lab A Chip"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1373\/clinchem.2013.206805","article-title":"Flexible Micro Spring Array Device for High-Throughput Enrichment of Viable Circulating Tumor Cells","volume":"60","author":"Harouaka","year":"2014","journal-title":"Clin. 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