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Moreover, the flow of blood through splenic red pulp and through artificial organs is associated with brief exposure to even higher levels of shear stress, reaching up to hundreds of Pa. As a result of this exposure, some properties of the cytosol, the cytoskeleton, and the cell membrane may be significantly affected. In this review, we aim to systematize the available information on RBC response to shear stress by focusing on reported changes in various red cell properties. We pay special attention to the results obtained using microfluidics, since these devices allow the researcher to accurately simulate blood flow conditions in the capillaries and spleen.<\/jats:p>","DOI":"10.3390\/mi16030259","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T12:01:17Z","timestamp":1740484877000},"page":"259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mechanical Stimulation of Red Blood Cells Aging: Focusing on the Microfluidics Application"],"prefix":"10.3390","volume":"16","author":[{"given":"Alexander","family":"Gural","sequence":"first","affiliation":[{"name":"Blood Bank, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9663-6916","authenticated-orcid":false,"given":"Ivana","family":"Paji\u0107-Lijakovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6588-7229","authenticated-orcid":false,"given":"Gregory","family":"Barshtein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The Faculty of Medicine, Hebrew University, Jerusalem 91120, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1016\/j.bpj.2017.05.052","article-title":"Prospects for Human Erythrocyte Skeleton-Bilayer Dissociation during Splenic Flow","volume":"113","author":"Zhu","year":"2017","journal-title":"Biophys. 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