{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:29:13Z","timestamp":1776054553226,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&amp;D Program of China","award":["Grants 2023YFF0721500"],"award-info":[{"award-number":["Grants 2023YFF0721500"]}]},{"name":"the National Key R&amp;D Program of China","award":["YN202514"],"award-info":[{"award-number":["YN202514"]}]},{"name":"the National Key R&amp;D Program of China","award":["Grants 62105177"],"award-info":[{"award-number":["Grants 62105177"]}]},{"name":"the National Key R&amp;D Program of China","award":["Grant 2023QNRC001"],"award-info":[{"award-number":["Grant 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Scientists Sponsorship Program of CAST","award":["Grants 2023YFF0721500"],"award-info":[{"award-number":["Grants 2023YFF0721500"]}]},{"name":"the Young Elite Scientists Sponsorship Program of CAST","award":["YN202514"],"award-info":[{"award-number":["YN202514"]}]},{"name":"the Young Elite Scientists Sponsorship Program of CAST","award":["Grants 62105177"],"award-info":[{"award-number":["Grants 62105177"]}]},{"name":"the Young Elite Scientists Sponsorship Program of CAST","award":["Grant 2023QNRC001"],"award-info":[{"award-number":["Grant 2023QNRC001"]}]},{"name":"the Young Elite Scientists Sponsorship Program of CAST","award":["Grant 2024NSCQMSX3784"],"award-info":[{"award-number":["Grant 2024NSCQMSX3784"]}]},{"name":"the Young Elite Scientists Sponsorship Program of CAST","award":["OEMT-2024-KF-03"],"award-info":[{"award-number":["OEMT-2024-KF-03"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["Grants 2023YFF0721500"],"award-info":[{"award-number":["Grants 2023YFF0721500"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["YN202514"],"award-info":[{"award-number":["YN202514"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["Grants 62105177"],"award-info":[{"award-number":["Grants 62105177"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["Grant 2023QNRC001"],"award-info":[{"award-number":["Grant 2023QNRC001"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["Grant 2024NSCQMSX3784"],"award-info":[{"award-number":["Grant 2024NSCQMSX3784"]}]},{"name":"the Natural Science Foundation of Chongqing","award":["OEMT-2024-KF-03"],"award-info":[{"award-number":["OEMT-2024-KF-03"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["Grants 2023YFF0721500"],"award-info":[{"award-number":["Grants 2023YFF0721500"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["YN202514"],"award-info":[{"award-number":["YN202514"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["Grants 62105177"],"award-info":[{"award-number":["Grants 62105177"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["Grant 2023QNRC001"],"award-info":[{"award-number":["Grant 2023QNRC001"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["Grant 2024NSCQMSX3784"],"award-info":[{"award-number":["Grant 2024NSCQMSX3784"]}]},{"name":"the State Key Laboratory of Optoelectronic Materials and Technologies","award":["OEMT-2024-KF-03"],"award-info":[{"award-number":["OEMT-2024-KF-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.<\/jats:p>","DOI":"10.3390\/mi16070785","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T10:45:08Z","timestamp":1751366708000},"page":"785","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform"],"prefix":"10.3390","volume":"16","author":[{"given":"Dongshuo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanqi","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanzhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingchen","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fucun","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9772-7286","authenticated-orcid":false,"given":"Guowei","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongxin","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuekai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.jss.2020.07.010","article-title":"AT deficiency in trauma and surgical critical care","volume":"256","author":"Ehrhardt","year":"2020","journal-title":"J. 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