{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:11:45Z","timestamp":1785291105822,"version":"3.55.0"},"reference-count":65,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402737"],"award-info":[{"award-number":["82402737"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"2024 High-quality Development Research Project of Shenzhen Bao\u2019an Public Hospital","award":["BAGZL2024134"],"award-info":[{"award-number":["BAGZL2024134"]}]},{"name":"2024 High-quality Development Research Project of Shenzhen Bao\u2019an Public Hospital","award":["BAGZL2024135"],"award-info":[{"award-number":["BAGZL2024135"]}]},{"name":"International Cooperation Base of Infrared Reflection Liquid Crystal Polymers and Device","award":["2015B050501010"],"award-info":[{"award-number":["2015B050501010"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZZYSM202206005"],"award-info":[{"award-number":["SZZYSM202206005"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Micromachines"],"abstract":"<jats:p>Although significant progress has been made in the global medical level, cardiovascular diseases still pose a serious threat to human life and health. Among many cardiovascular diseases, acute myocardial infarction (AMI) is particularly severe. If not treated in a timely manner, it may lead to serious consequences such as cardiac arrest and sudden death. Early diagnosis of myocardial infarction (MI) is an important means of preventing and controlling the mortality rate of AMI. Creatine kinase isoenzyme (CK-MB) is a key biomarker of MI. It rises rapidly within 2 h after myocardial injury, reaches its peak at 24 h, and returns to normal at 72 h. Furthermore, CK-MB has a high specificity in monitoring secondary MI. Therefore, the early, real-time, and accurate detection of CK-MB is of great significance for the prevention, diagnosis, and prognosis of AMI. Conventional CK-MB detection methods have problems such as false positive elevation, large blood sampling volume, long time consumption, and complex operation, making it difficult to meet the needs of point-of-care testing (POCT). Biosensor technology, with its low cost, high sensitivity, and portability, offers a promising solution for point-of-care CK-MB testing, thereby greatly aiding AMI diagnosis.<\/jats:p>","DOI":"10.3390\/mi16101111","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T10:49:34Z","timestamp":1759142974000},"page":"1111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Research Progress of Biosensing Technology in the Detection of Creatine Kinase Isoenzyme MB"],"prefix":"10.3390","volume":"16","author":[{"given":"Qixing","family":"Pan","sequence":"first","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingliang","family":"Jin","sequence":"additional","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liang","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"},{"name":"Department of Pharmacy, Shenzhen Bao\u2019an Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxia","family":"Lin","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"},{"name":"Department of Pharmacy, Shenzhen Bao\u2019an Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yechu","family":"Dai","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"},{"name":"Department of Pharmacy, Shenzhen Bao\u2019an Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4509-0201","authenticated-orcid":false,"given":"Zhenping","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Shui","sequence":"additional","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamei","family":"Chen","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"},{"name":"Department of Pharmacy, Shenzhen Bao\u2019an Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/1475-6773.13805","article-title":"Providers\u2019 knowledge of diagnosis and treatment best practices for acute myocardial infarction (AMI): Evidence from India using clinical vignettes","volume":"56","author":"Kalita","year":"2021","journal-title":"Health Serv. 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