{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:23:21Z","timestamp":1790148201307,"version":"4.0.1"},"reference-count":122,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402737"],"award-info":[{"award-number":["82402737"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2023A1515110376"],"award-info":[{"award-number":["2023A1515110376"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"2024 High-quality Development Research Project of Shenzhen Bao\u2019an Public Hospital","award":["BAGZL2024134"],"award-info":[{"award-number":["BAGZL2024134"]}]},{"name":"2024 High-quality Development Research Project of Shenzhen Bao\u2019an Public Hospital","award":["BAGZL2024135"],"award-info":[{"award-number":["BAGZL2024135"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZZYSM202206005"],"award-info":[{"award-number":["SZZYSM202206005"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>As a class of self-organized soft matter systems merging fluidic mobility with long-range molecular order, cholesteric liquid crystals (CLCs) possess immense potential for the development of high-sensitivity, visually tractable flexible sensors. Leveraging their unique helical superstructures and stimuli-responsive photonic bandgaps, CLCs can transduce subtle physical or chemical perturbations into discernible optical signatures, such as Bragg reflection shifts or mesomorphic textural transitions. Nonetheless, the intrinsic fluidity of CLCs often compromises their structural integrity, while conventional one-dimensional (1D) or two-dimensional (2D) confinement geometries exhibit pronounced angular dependence, significantly constraining their detection precision in complex environments. Recently, microfluidic technology has emerged as a pivotal paradigm for achieving sophisticated three-dimensional (3D) spatial confinement of CLCs through the precise manipulation of microscale fluid volumes. This review systematically delineates recent advancements in microfluidics-enabled CLC sensors. Initially, the fundamental self-assembly principles and optical properties of CLCs are introduced, emphasizing the unique advantages of 3D spherical confinement in mitigating angular sensitivity and intensifying interfacial interactions. Subsequently, the primary sensing mechanisms are bifurcated into bulk-driven sensing via pitch modulation and interface-driven sensing via topological configuration transitions. We then detail the microfluidic-based fabrication strategies and engineering protocols for diverse 3D architectures, including monodisperse\/multiphase droplets, microcapsules, shells, and Janus structures. Building upon these structural frameworks, current sensing applications in physical (temperature, strain\/stress), chemical (volatile organic compounds, ions, pH), and biological (biomarkers, pathogens) detection are evaluated. Lastly, in light of persistent challenges, such as intricate signal interpretation and limited robustness in complex matrices, we propose future research trajectories, encompassing the co-optimization of geometric parameters (size and curvature), artificial intelligence-enhanced automated diagnostics, and multi-field-coupled intelligent integration. This work seeks to provide a comprehensive roadmap for the design of next-generation, high-performance, and portable liquid-state photonic sensing platforms.<\/jats:p>","DOI":"10.3390\/mi17020244","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:09:32Z","timestamp":1770998972000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microfluidics-Assisted Three-Dimensional Confinement of Cholesteric Liquid Crystals for Sensing Applications"],"prefix":"10.3390","volume":"17","author":[{"given":"Jiamei","family":"Chen","sequence":"first","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Feng","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Huang","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijian","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongbing","family":"Wu","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lvqin","family":"Qiu","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Cao","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liang","sequence":"additional","affiliation":[{"name":"The Seventh Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Shenzhen 518133, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2310505","DOI":"10.1002\/adma.202310505","article-title":"Age of flexible electronics: Emerging trends in soft multifunctional sensors","volume":"36","author":"Lee","year":"2024","journal-title":"Adv. Mater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2401503","DOI":"10.1002\/adhm.202401503","article-title":"A review of conductive hydrogel-based wearable temperature sensors","volume":"13","author":"Mo","year":"2024","journal-title":"Adv. Healthc. Mater."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2303471","DOI":"10.1002\/adfm.202303471","article-title":"Metal-organic framework reinforced highly stretchable and durable conductive hydrogel-based triboelectric nanogenerator for biomotion sensing and wearable human-machine interfaces","volume":"33","author":"Rahman","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2212202","DOI":"10.1002\/adma.202212202","article-title":"Soft multimaterial magnetic fibers and textiles","volume":"35","author":"Banerjee","year":"2023","journal-title":"Adv. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1021\/acsnano.3c09307","article-title":"Soft fiber\/textile actuators: From design strategies to diverse applications","volume":"18","author":"Xue","year":"2023","journal-title":"ACS Nano"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Patern\u00f2, L., and Lorenzon, L. (2023). Soft robotics in wearable and implantable medical applications: Translational challenges and future outlooks. Front. Robot. AI, 10.","DOI":"10.3389\/frobt.2023.1075634"},{"key":"ref_7","first-page":"102506","article-title":"4D printing of patterned multimaterial magnetic hydrogel actuators","volume":"49","author":"Junka","year":"2022","journal-title":"Addit. Manuf."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/anie.199208421","article-title":"Soft matter (Nobel lecture)","volume":"31","year":"1992","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"032003","DOI":"10.1088\/2631-7990\/ada857","article-title":"Manufacturing high-performance flexible sensors via advanced patterning techniques","volume":"7","author":"Qin","year":"2025","journal-title":"Int. J. Extrem. Manuf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41377-022-00930-5","article-title":"Self-assembled liquid crystal architectures for soft matter photonics","volume":"11","author":"Qin","year":"2022","journal-title":"Light Sci. Appl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1800335","DOI":"10.1002\/adom.201800335","article-title":"Cholesteric liquid crystal materials for tunable diffractive optics","volume":"6","author":"Ryabchun","year":"2018","journal-title":"Adv. Opt. Mater."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.jcis.2024.08.036","article-title":"Unveiling the structural influence of nematic mesogens on customizable temperature and spectral responses","volume":"677","author":"Oh","year":"2025","journal-title":"J. Colloid Interface Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yi, H., Lee, S.H., Kim, D., Jeong, H.E., and Jeong, C. (2022). Colorimetric sensor based on hydroxypropyl cellulose for wide temperature sensing range. Sensors, 22.","DOI":"10.3390\/s22030886"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2412298","DOI":"10.1002\/adfm.202412298","article-title":"Cholesteric liquid crystal elastomer coatings with brilliant structural colors and mechanochromic response fabricated by spray deposition","volume":"35","author":"Li","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2798","DOI":"10.1039\/D3LC00223C","article-title":"Strain-induced recognition of molecular and chirality in cholesteric liquid crystal droplets for distance and curvature sensing","volume":"23","author":"Xie","year":"2023","journal-title":"Lab Chip"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2107205","DOI":"10.1002\/adma.202107205","article-title":"Tackling humidity with designer ionic liquid-based gas sensing soft materials","volume":"34","author":"Esteves","year":"2022","journal-title":"Adv. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"16764","DOI":"10.1021\/acsami.2c03420","article-title":"Humidity-responsive photonic crystals with pH and SO2 gas detection ability based on cholesteric liquid crystalline networks","volume":"14","author":"Shen","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.jcis.2024.05.194","article-title":"Optically responsive dry cholesteric liquid crystal marbles","volume":"671","author":"Kocaman","year":"2024","journal-title":"J. Colloid Interface Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1706512","DOI":"10.1002\/adma.201706512","article-title":"Stimuli-driven control of the helical axis of self-organized soft helical superstructures","volume":"30","author":"Bisoyi","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1039\/D1TC04395A","article-title":"Development in liquid crystal microcapsules: Fabrication, optimization and applications","volume":"10","author":"Qu","year":"2022","journal-title":"J. Mater. Chem. C"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4887","DOI":"10.1021\/acs.chemrev.1c00761","article-title":"Liquid crystals: Versatile self-organized smart soft materials","volume":"122","author":"Bisoyi","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1007\/s13233-018-6148-3","article-title":"Controlled encapsulation of cholesteric liquid crystals using emulsion templates","volume":"26","author":"Lee","year":"2018","journal-title":"Macromol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2100016","DOI":"10.1002\/adpr.202100016","article-title":"Temperature-responsive photonic devices based on cholesteric liquid crystals","volume":"2","author":"Zhang","year":"2021","journal-title":"Adv. Photonics Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6260","DOI":"10.1002\/adma.201202913","article-title":"Cholesteric liquid crystals with a broad light reflection band","volume":"24","author":"Mitov","year":"2012","journal-title":"Adv. Mater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"133003","DOI":"10.1088\/1361-648X\/aa5706","article-title":"Liquid crystals in micron-scale droplets, shells and fibers","volume":"29","author":"Urbanski","year":"2017","journal-title":"J. Phys. Condens. Matter"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"11982","DOI":"10.1039\/c2sm27048j","article-title":"Geometrical frustration of chiral ordering in cholesteric droplets","volume":"8","author":"Porenta","year":"2012","journal-title":"Soft Matter"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2305018","DOI":"10.3866\/PKU.WHXB202305018","article-title":"Dynamic manipulation of photonic bandgaps in cholesteric liquid crystal microdroplets for applications","volume":"40","author":"Liu","year":"2024","journal-title":"Acta Phys. Chim. Sin."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.1021\/ar500249k","article-title":"Light-directing chiral liquid crystal nanostructures: From 1D to 3D","volume":"47","author":"Bisoyi","year":"2014","journal-title":"Acc. Chem. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"14376","DOI":"10.1021\/acsami.9b02680","article-title":"Patterned full-color reflective coatings based on photonic cholesteric liquid-crystalline particles","volume":"11","author":"Belmonte","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.jiec.2018.08.014","article-title":"2-Dimensional colloidal micropatterning of cholesteric liquid crystal microcapsules for temperature-responsive color displays","volume":"68","author":"Lee","year":"2018","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2507000","DOI":"10.1002\/adma.202507000","article-title":"Electro-thermo cooperative responsiveness of cholesteric heliconical photonics architectures featuring adaptative sensitivity","volume":"37","author":"Yuan","year":"2025","journal-title":"Adv. Mater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.molliq.2016.11.077","article-title":"Electric behaviour of a Schiff\u2019s base liquid crystal compound doped with a low concentration of BaTiO3 nanoparticles","volume":"225","author":"Dubey","year":"2017","journal-title":"J. Mol. Liq."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1907626","DOI":"10.1002\/smll.201907626","article-title":"Structural color materials for optical anticounterfeiting","volume":"16","author":"Hong","year":"2020","journal-title":"Small"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"25512","DOI":"10.1021\/acsnano.4c05320","article-title":"Magneto-responsive chiral optical materials: Flow-induced twisting of cellulose nanocrystals in patterned magnetic fields","volume":"18","author":"Kim","year":"2024","journal-title":"ACS Nano"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5362","DOI":"10.1039\/D4TC00726C","article-title":"Phototuning structural color and optical switching cholesteric textures in azobenzene-doped cholesteric liquid crystals","volume":"12","author":"Lu","year":"2024","journal-title":"J. Mater. Chem. C"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.1021\/acs.langmuir.1c00256","article-title":"Liquid crystals in curved confined geometries: Microfluidics bring new capabilities for photonic applications and beyond","volume":"37","author":"Chen","year":"2021","journal-title":"Langmuir"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2204275","DOI":"10.1002\/adma.202204275","article-title":"Liquid Crystalline systems from nature and interaction of living organisms with liquid crystals","volume":"35","author":"Kim","year":"2023","journal-title":"Adv. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1801335","DOI":"10.1002\/adma.201801335","article-title":"Nature-inspired emerging chiral liquid crystal nanostructures: From molecular self-assembly to DNA mesophase and nanocolloids","volume":"32","author":"Wang","year":"2020","journal-title":"Adv. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Melton, C., Riahinasab, S., Keshavarz, A., Stokes, B., and Hirst, L. (2018). Phase transition-driven nanoparticle assembly in liquid crystal droplets. Nanomaterials, 8.","DOI":"10.3390\/nano8030146"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2111280","DOI":"10.1002\/adfm.202111280","article-title":"Liquid crystal templated chiral plasmonic films with dynamic tunability and moldability","volume":"32","author":"Grzelak","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Wu, P.C., Pai, C.P., Lee, M.J., and Lee, W. (2021). A single-substrate biosensor with spin-coated liquid crystal film for simple, sensitive and label-free protein detection. Biosensors, 11.","DOI":"10.3390\/bios11100374"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7184","DOI":"10.1039\/C8TC02252F","article-title":"An easily coatable temperature responsive cholesteric liquid crystal oligomer for making structural colour patterns","volume":"6","author":"Zhang","year":"2018","journal-title":"J. Mater. Chem. C"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zhang, L., Cui, Y., Wang, Q., Zhou, H., Wang, H., Li, Y., Yang, Z., Cao, H., Wang, D., and He, W. (2022). Spatial patterning of fluorescent liquid crystal ink based on inkjet printing. Molecules, 27.","DOI":"10.3390\/molecules27175536"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"40801","DOI":"10.1021\/acsomega.4c05664","article-title":"3D Printing of Thermally Responsive Shape Memory Liquid Crystalline Epoxy Networks","volume":"9","author":"Pekol","year":"2024","journal-title":"ACS Omega"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"31919","DOI":"10.1039\/C6RA05024G","article-title":"Wavelength-tuneable laser emission from stretchable chiral nematic liquid crystal gels via in situ photopolymerization","volume":"6","author":"Wood","year":"2016","journal-title":"RSC Adv."},{"key":"ref_46","first-page":"14800","article-title":"Electrospun core-sheath fibers with a uniformly aligned polymer network liquid crystal (PNLC)","volume":"15","author":"Zhang","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"8879","DOI":"10.1039\/D5SM00591D","article-title":"Metastable structures in electrospun microfibers with a long-pitch chiral nematic liquid crystal core","volume":"21","author":"Thum","year":"2025","journal-title":"Soft Matter"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2422176","DOI":"10.1002\/adfm.202422176","article-title":"High-speed and scalable wet spinning of graphene\/liquid crystalline elastomer composite filaments","volume":"35","author":"Martinez","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1021\/acsomega.8b03189","article-title":"Effect of liquid crystalline texture of mesophase pitches on the structure and property of large-diameter carbon fibers","volume":"4","author":"Yuan","year":"2019","journal-title":"ACS Omega"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2001828","DOI":"10.1002\/adom.202001828","article-title":"Photonic NO2 gas sensing with binaphthyl-Based dopants","volume":"9","author":"Spengler","year":"2021","journal-title":"Adv. Opt. Mater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"35302","DOI":"10.1021\/acsami.3c07287","article-title":"Recyclable cholesteric phase liquid crystal device for detecting storage temperature failure","volume":"15","author":"Spengler","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3926","DOI":"10.1021\/acs.analchem.4c05166","article-title":"Colorimetric, quantitative, and portable liquid crystal elastomer biosensing of cholesterol and malathion","volume":"97","author":"Mu","year":"2025","journal-title":"Anal. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"126894","DOI":"10.1016\/j.snb.2019.126894","article-title":"Photonic calcium and humidity array sensor prepared with reactive cholesteric liquid crystal mesogens","volume":"298","author":"Myung","year":"2019","journal-title":"Sens. Actuators B"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Yeh, T.Y., Liu, M.F., Lin, R.D., and Hwang, S.J. (2022). Alcohol selective optical sensor based on porous cholesteric liquid crystal polymer networks. Molecules, 27.","DOI":"10.3390\/molecules27030773"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"123574","DOI":"10.1016\/j.molliq.2023.123574","article-title":"Transparent core-sheath composite fibers as flexible temperature sensor based on liquid crystal color change for smart sportswear","volume":"393","author":"Fu","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Zhao, H., Li, R., Li, H., Jin, L., Lan, Y., Jiang, S., and Liu, M. (2025). Reprogrammable thermochromic fiber actuators for spatial temperature indication devices. Adv. Funct. Mater., e16121.","DOI":"10.1002\/adfm.202516121"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Wang, I.T., Lee, Y.H., Chuang, E.Y., and Hsiao, Y.C. (2021). Sensitive, color-indicating and labeling-free multi-detection cholesteric liquid crystal biosensing chips for detecting albumin. Polymers, 13.","DOI":"10.3390\/polym13091463"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"16830","DOI":"10.1021\/acsami.4c00920","article-title":"Photonic interpenetrating polymer network fibers comprising intertwined solid-state cholesteric liquid crystal and polyelectrolyte networks for sensor applications","volume":"16","author":"Adane","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nature05058","article-title":"The origins and the future of microfluidics","volume":"442","author":"Whitesides","year":"2006","journal-title":"Nature"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"7964","DOI":"10.1021\/acs.chemrev.6b00848","article-title":"Emerging droplet microfluidics","volume":"117","author":"Shang","year":"2017","journal-title":"Chem. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0925-4005(90)80209-I","article-title":"Miniaturized total chemical analysis systems: A novel concept for chemical sensing","volume":"1","author":"Manz","year":"1990","journal-title":"Sens. Actuators B"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1126\/science.288.5463.113","article-title":"Monolithic microfabricated valves and pumps by multilayer soft lithography","volume":"288","author":"Unger","year":"2000","journal-title":"Science"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1126\/science.1076996","article-title":"Microfluidic large-scale integration","volume":"298","author":"Thorsen","year":"2002","journal-title":"Science"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"0856","DOI":"10.34133\/research.0856","article-title":"Innovative advances in droplet microfluidics","volume":"8","author":"Zhang","year":"2025","journal-title":"Research"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Song, C., and Tan, S.H. (2017). A perspective on the rise of optofluidics and the future. Micromachines, 8.","DOI":"10.3390\/mi8050152"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/nphoton.2011.163","article-title":"The photonic integration of non-solid media using optofluidics","volume":"5","author":"Schmidt","year":"2011","journal-title":"Nat. Photonics"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/nature05060","article-title":"Developing optofluidic technology through the fusion of microfluidics and optics","volume":"442","author":"Psaltis","year":"2006","journal-title":"Nature"},{"key":"ref_68","first-page":"10001","article-title":"Light confinement in liquid crystals for optofluidic integrated microsystems-INVITED[C]\/\/EPJ Web of Conferences","volume":"255","author":"Kumaran","year":"2021","journal-title":"EDP Sci."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Deng, J., Han, D., and Yang, J. (2021). Applications of microfluidics in liquid crystal-based biosensors. Biosensors, 11.","DOI":"10.3390\/bios11100385"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"5305","DOI":"10.1021\/ja1095254","article-title":"Microfluidic synthesis of highly shape-anisotropic particles from liquid crystalline elastomers with defined director field configurations","volume":"133","author":"Ohm","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/21680396.2014.963716","article-title":"Liquid crystal microfluidics: Surface, elastic and viscous interactions at microscales","volume":"2","author":"Sengupta","year":"2014","journal-title":"Liq. Cryst. Rev."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1039\/c3lc50501d","article-title":"Optofluidic-tunable color filters and spectroscopy based on liquid-crystal microflows","volume":"13","author":"Cuennet","year":"2013","journal-title":"Lab Chip"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s43074-021-00041-1","article-title":"Liquid crystal-amplified optofluidic biosensor for ultra-highly sensitive and stable protein assay","volume":"2","author":"Wang","year":"2021","journal-title":"PhotoniX"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s00604-024-06654-0","article-title":"Dimension compensation of printed master molds by a desktop LCD 3D printer for high-precision microfluidic applications","volume":"191","author":"Zhang","year":"2024","journal-title":"Microchim. Acta"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"113926","DOI":"10.1016\/j.sna.2022.113926","article-title":"Microfluidic devices for the detection of contamination in water samples: A review","volume":"347","author":"Rai","year":"2022","journal-title":"Sens. Actuators A"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1039\/C8LC01291A","article-title":"Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides","volume":"19","author":"Bao","year":"2019","journal-title":"Lab Chip"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"eabp9245","DOI":"10.1126\/sciadv.abp9245","article-title":"High-efficiency quantitative control of mitochondrial transfer based on droplet microfluidics and its application on muscle regeneration","volume":"8","author":"Sun","year":"2022","journal-title":"Sci. Adv."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.1039\/C4LC00698D","article-title":"Double emulsions from a capillary array injection microfluidic device","volume":"14","author":"Shang","year":"2014","journal-title":"Lab Chip"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1039\/C6LC00809G","article-title":"Microfluidic production of multiple emulsions and functional microcapsules","volume":"16","author":"Lee","year":"2016","journal-title":"Lab Chip"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.1039\/D2LC00196A","article-title":"Recent advances in the microfluidic production of functional microcapsules by multiple-emulsion templating","volume":"22","author":"Kim","year":"2022","journal-title":"Lab Chip"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2301406","DOI":"10.1002\/smtd.202301406","article-title":"Droplet microfluidic devices: Working principles, fabrication methods, and scale-up applications","volume":"8","author":"Ma","year":"2024","journal-title":"Small Methods"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1805033","DOI":"10.1002\/adma.201805033","article-title":"Microfluidics-based biomaterials and biodevices","volume":"31","author":"Dong","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Niculescu, A.G., Chircov, C., B\u00eerc\u0103, A.C., and Grumezescu, A.M. (2021). Fabrication and applications of microfluidic devices: A review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22042011"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.snb.2013.03.117","article-title":"Deformable mold based on-demand microchannel fabrication technology","volume":"183","author":"Yu","year":"2013","journal-title":"Sens. Actuators B"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1126\/science.1109164","article-title":"Monodisperse double emulsions generated from a microcapillary device","volume":"308","author":"Utada","year":"2005","journal-title":"Science"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1801084","DOI":"10.1002\/adhm.201801084","article-title":"Paper microfluidics for cell analysis","volume":"8","author":"Ma","year":"2019","journal-title":"Adv. Healthc. Mater."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1039\/C6LC00163G","article-title":"The upcoming 3D-printing revolution in microfluidics","volume":"16","author":"Bhattacharjee","year":"2016","journal-title":"Lab Chip"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1900275","DOI":"10.1002\/admt.201900275","article-title":"Functional 3D printing for microfluidic chips","volume":"4","author":"Weisgrab","year":"2019","journal-title":"Adv. Mater. Technol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1903940","DOI":"10.1002\/smll.201903940","article-title":"Microfluidics for biosynthesizing: From droplets and vesicles to artificial cells","volume":"16","author":"Ai","year":"2020","journal-title":"Small"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"4163","DOI":"10.1103\/PhysRevLett.86.4163","article-title":"Dynamic pattern formation in a vesicle-generating microfluidic device","volume":"86","author":"Thorsen","year":"2001","journal-title":"Phys. Rev. Lett."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"5646","DOI":"10.1039\/C7CS00263G","article-title":"Microfluidic fabrication of microparticles for biomedical applications","volume":"47","author":"Li","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1021\/la990101e","article-title":"Monodisperse emulsion generation via drop break off in a coflowing stream","volume":"16","author":"Umbanhowar","year":"2000","journal-title":"Langmuir"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.snb.2017.08.152","article-title":"Monodisperse water-in-oil-in-water emulsions generation for synthesising alginate hydrogel microspheres via locally hydrophobic modification to PMMA microchannels","volume":"255","author":"Li","year":"2018","journal-title":"Sens. Actuators B"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"9905","DOI":"10.1021\/la0480336","article-title":"Controlled production of monodisperse double emulsions by two-step droplet breakup in microfluidic devices","volume":"20","author":"Okushima","year":"2004","journal-title":"Langmuir"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"8890","DOI":"10.1002\/ange.201102946","article-title":"One-step emulsification of multiple concentric shells with capillary microfluidic devices","volume":"123","author":"Kim","year":"2011","journal-title":"Angew. Chem."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"129165","DOI":"10.1016\/j.snb.2020.129165","article-title":"Functional solid-state photonic droplets with interpenetrating polymer network and their applications to biosensors","volume":"329","author":"Lim","year":"2021","journal-title":"Sens. Actuators B"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2107275","DOI":"10.1002\/adfm.202107275","article-title":"Thermochromic microcapsules containing chiral mesogens enclosed by hydrogel shell for colorimetric temperature reporters","volume":"32","author":"Kim","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"20350","DOI":"10.1021\/acsami.9b04105","article-title":"Optical properties and applications of photonic shells","volume":"11","author":"Myung","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"2002166","DOI":"10.1002\/adma.202002166","article-title":"Photonic multishells composed of cholesteric liquid crystals designed by controlled phase separation in emulsion drops","volume":"32","author":"Park","year":"2020","journal-title":"Adv. Mater."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"2207985","DOI":"10.1002\/adma.202207985","article-title":"Janus microdroplets with tunable self-recoverable and switchable reflective structural colors","volume":"35","author":"Liu","year":"2023","journal-title":"Adv. Mater."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.bios.2016.04.069","article-title":"Liquid crystals based sensing platform-technological aspects","volume":"85","author":"Hussain","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"8679","DOI":"10.1039\/c2sm25383f","article-title":"Morphological transitions in liquid crystal nanodroplets","volume":"8","author":"Tomar","year":"2012","journal-title":"Soft Matter"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1039\/D5ME00070J","article-title":"Curved confinement directs anchoring-mediated structural transitions in highly chiral liquid crystal shells","volume":"10","author":"Norouzi","year":"2025","journal-title":"Mol. Syst. Des. Eng."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"26407","DOI":"10.1021\/acsami.6b09624","article-title":"Cholesteric liquid crystal droplets for biosensors","volume":"8","author":"Lee","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1039\/C6LC01018K","article-title":"Passive and active droplet generation with microfluidics: A review","volume":"17","author":"Zhu","year":"2017","journal-title":"Lab Chip"},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Wang, H., Xu, T., Fu, Y., Wang, Z., Leeson, M.S., Jiang, J., and Liu, T. (2022). Liquid crystal biosensors: Principles, structure and applications. Biosensors, 12.","DOI":"10.3390\/bios12080639"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"47342","DOI":"10.1021\/acsami.0c15718","article-title":"Optical multisensor array with functionalized photonic droplets by an interpenetrating polymer network for human blood analysis","volume":"12","author":"Kim","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"124776","DOI":"10.1016\/j.talanta.2023.124776","article-title":"Microfluidic based single cell or droplet manipulation: Methods and applications","volume":"265","author":"Lan","year":"2023","journal-title":"Talanta"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1039\/b708091c","article-title":"Surface-induced droplet fusion in microfluidic devices","volume":"7","author":"Fidalgo","year":"2007","journal-title":"Lab Chip"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"128728","DOI":"10.1016\/j.colsurfa.2022.128728","article-title":"Label-free optical sensor based on liquid crystal sessile droplet array for penicillin G determination","volume":"644","author":"Xie","year":"2022","journal-title":"Colloids Surf. A"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"10757","DOI":"10.1021\/acs.analchem.9b02288","article-title":"Forming a large-scale droplet array in a microcage array chip for high-throughput screening","volume":"91","author":"Xu","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Luan, C., Luan, H., and Luo, D. (2020). Application and technique of liquid crystal-based biosensors. Micromachines, 11.","DOI":"10.3390\/mi11020176"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"2202141","DOI":"10.1002\/adom.202202141","article-title":"Microfluidic construction of responsive photonic microcapsules of cholesteric liquid crystal for colorimetric temperature microsensors","volume":"11","author":"Pan","year":"2023","journal-title":"Adv. Opt. Mater."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"13991","DOI":"10.1039\/D1TC02801D","article-title":"Detection of bile acids using optical biosensors based on cholesteric liquid crystal droplets","volume":"9","author":"Gollapelli","year":"2021","journal-title":"J. Mater. Chem. C"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"9177","DOI":"10.1021\/jacs.1c04115","article-title":"Complex liquid crystal emulsions for biosensing","volume":"143","author":"Fong","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1007\/s40843-021-2014-0","article-title":"Integrated sensing from the synergetic color change of the center\/brush of cholesteric liquid crystal particles","volume":"65","author":"Shang","year":"2022","journal-title":"Sci. China Mater."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1002\/adma.201403271","article-title":"Robust microfluidic encapsulation of cholesteric liquid crystals toward photonic ink capsules","volume":"27","author":"Lee","year":"2014","journal-title":"Adv. Mater."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.1039\/C4TC00699B","article-title":"Chemiluminescence emission in cholesteric liquid crystalline core\u2013shell microcapsules","volume":"2","author":"Iwai","year":"2014","journal-title":"J. Mater. Chem. C"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"122447","DOI":"10.1016\/j.molliq.2023.122447","article-title":"Optical detection of bovine serum albumin using charged cholesteric liquid crystal droplets functionalized with surfactant","volume":"386","author":"Pradhan","year":"2023","journal-title":"J. Mol. Liq."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Qu, X., Ma, J., Zeng, D., Luo, J., Wu, J., Liu, C., Deng, Z., Chen, L., Han, R., and Qiao, Y. (2026). Microcavity-assisted microfluidic physical sensors: Materials, structures, and multifunctional applications. Lab Chip.","DOI":"10.1039\/D5LC00822K"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"6847","DOI":"10.1021\/acs.analchem.4c06803","article-title":"Acoustofluidics: Technology Advances and Applications from 2022 to 2024","volume":"97","author":"Godary","year":"2025","journal-title":"Anal. Chem."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.1039\/c3lc50361e","article-title":"Surface acoustic wave microfluidics","volume":"13","author":"Ding","year":"2013","journal-title":"Lab Chip"}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/17\/2\/244\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T05:29:31Z","timestamp":1771133371000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/17\/2\/244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,13]]},"references-count":122,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["mi17020244"],"URL":"https:\/\/doi.org\/10.3390\/mi17020244","relation":{},"ISSN":["2072-666X"],"issn-type":[{"value":"2072-666X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,13]]}}}