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Here, we developed an Open-access Dialysis Membrane-integrated Microfluidic Device (O-DMiMD) that uses molecular weight-dependent transport across a dialysis membrane to decouple nutrient supply from drug exposure control. The device comprises a cell culture compartment (CCC) and a donor compartment (DC) separated by a dialysis membrane. Transport functions were evaluated using Lucifer Yellow, FITC-dextran, and glucose, followed by drug-response studies using SN-38 and T-DM1 under different medium change conditions. Lucifer Yellow and glucose permeated through the dialysis membrane, whereas FITC-dextran was retained. DC medium change supplied glucose to the CCC and maintained A549\/HepG2 co-culture proliferation comparably to direct CCC medium replacement. For SN-38, partial transport to the DC and retention in the CCC generated time-dependent exposure profiles; in A549\/HepaRG co-culture, medium change conditions altered A549 viability. For T-DM1, conditions with or without re-addition to the CCC produced different SK-BR-3 responses, suggesting exposure-dependent effects for high-molecular-weight drugs. The O-DMiMD provides an open-access in vitro platform for evaluating drug responses under exposure profiles governed by molecular weights, protein binding, medium changes, and metabolic cell contexts.<\/jats:p>","DOI":"10.3390\/mi17070835","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T08:04:31Z","timestamp":1784016271000},"page":"835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Open-Access Dialysis Membrane-Integrated Microfluidic Device for Generating Drug Exposure Profiles Through Molecular-Weight-Dependent Transport"],"prefix":"10.3390","volume":"17","author":[{"given":"Hajime","family":"Miyashita","sequence":"first","affiliation":[{"name":"Graduate School of Science and Technology, Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3146-667X","authenticated-orcid":false,"given":"Kenta","family":"Shinha","sequence":"additional","affiliation":[{"name":"Micro\/Nano Technology Center (MNTC), Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Micro\/Nano Technology Center (MNTC), Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moeno","family":"Kadoguchi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakumamachi, Kanazawa 920-1192, Ishikawa, Japan"},{"name":"Department of Regulatory Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakumamachi, Kanazawa 920-1192, Ishikawa, Japan"},{"name":"Department of Regulatory Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0623-0248","authenticated-orcid":false,"given":"Hiroshi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology, Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan"},{"name":"Micro\/Nano Technology Center (MNTC), Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tuntland, T., Ethell, B., Kosaka, T., Blasco, F., Zang, R.X., Jain, M., Gould, T., and Hoffmaster, K. 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