{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:31:47Z","timestamp":1787056307260,"version":"build-2736575974"},"reference-count":187,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52175549"],"award-info":[{"award-number":["52175549"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["52175549"],"award-info":[{"award-number":["52175549"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Doctoral Initiation Project of Xichang University","award":["RCZ202606"],"award-info":[{"award-number":["RCZ202606"]}]},{"award":["RCZ202606"],"award-info":[{"award-number":["RCZ202606"]}],"id":[{"id":"https:\/\/ror.org\/02h3fyk31","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Science and Technology Bureau of Liangshan Yi Autonomous Prefecture"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>High-efficiency mixing and precise droplet generation are essential for the broad application of microfluidic chips in biology, chemistry, and medicine, with integrated micromixer and microdroplet generator systems playing an increasingly important role in diagnostics and drug detection. This review offers a comprehensive and systematic overview of micromixers and microdroplet generators, covering their classification, working principles, performance characterization, and integration strategies. Passive and active micromixers, the latter employing pressure, electric, acoustic, magnetic, and thermal fields to enhance mixing, are summarized with a critical discussion of their advantages, limitations, and structural optimization. Various droplet generation methods, including crossflow, flow focusing, coflow, step emulsification, and active techniques, are examined alongside key parameters affecting droplet size, monodispersity, and generation frequency. Performance characterization metrics for both components, including mixing efficiency, mixing time, pressure drop, droplet size distribution, generation frequency, and stability, are also discussed. The review then focuses on integration, emphasizing two primary architectural strategies: mixing reagents before encapsulation and inducing mixing within droplets after formation. Synergistic applications in single cell analysis, materials synthesis, and drug screening are presented. Notably, unlike previous reviews that treat micromixers or droplet generators separately, this review uniquely emphasizes architectural integration and critically evaluates the associated trade-offs. Current challenges and future directions, including material selection, fabrication techniques, and practical application-oriented considerations, are also addressed.<\/jats:p>","DOI":"10.3390\/mi17080963","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:42:17Z","timestamp":1786974137000},"page":"963","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Review on Micromixers, Microdroplet Generators and Their Integration"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3993-8472","authenticated-orcid":false,"given":"Wang","family":"He","sequence":"first","affiliation":[{"name":"College of Mechanical and Electrical Engineering, Xichang University, Xichang 615000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tribology Research Institute, School of Mechanical Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wu","sequence":"additional","affiliation":[{"name":"The 10th Research Institute of China Electronics Technology Corporation, Chengdu 610036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tribology, Tsinghua University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Chen","sequence":"additional","affiliation":[{"name":"Tribology Research Institute, School of Mechanical Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.trac.2020.116118","article-title":"Recent advances in microfluidic technology and applications for anti-cancer drug screening","volume":"134","author":"Shi","year":"2021","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/adfm.202113153","article-title":"Emerging nanoporous materials for biomolecule separation","volume":"32","author":"Song","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_3","first-page":"302","article-title":"Application of microfluidic chips in anticancer drug screening","volume":"22","author":"Fan","year":"2022","journal-title":"Bosn. J. Basic Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.talanta.2019.120477","article-title":"Three-gradient constructions in a flow-rate insensitive microfluidic system for drug screening towards personalized treatment","volume":"208","author":"Shen","year":"2020","journal-title":"Talanta"},{"key":"ref_5","first-page":"10","article-title":"Microfluidic fabrication of biomimetic helical hydrogel microfibers for blood-vessel-on-a-chip applications","volume":"8","author":"Jia","year":"2019","journal-title":"Adv. Healthc. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0925-4005(90)80209-I","article-title":"Miniaturized total chemical-analysis systems\u2014A novel concept for chemical sensing","volume":"1","author":"Manz","year":"1990","journal-title":"Sens. Actuator B Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1039\/D0LC01068E","article-title":"A high-throughput multiplexed microfluidic device for COVID-19 serology assays","volume":"21","year":"2021","journal-title":"Lab Chip"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1039\/D0LC01046D","article-title":"High-resolution integrated piezoresistive sensors for microfluidic monitoring","volume":"21","author":"Zhou","year":"2021","journal-title":"Lab Chip"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4528","DOI":"10.1039\/D0LC00881H","article-title":"Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies","volume":"20","author":"Wan","year":"2020","journal-title":"Lab Chip"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"803","DOI":"10.4252\/wjsc.v11.i10.803","article-title":"Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis","volume":"11","author":"Kim","year":"2019","journal-title":"World J. Stem Cells"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Song, K., Li, G., Zu, X., Du, Z., Liu, L., and Hu, Z. (2020). The fabrication and application mechanism of microfluidic systems for high throughput biomedical screening: A review. Micromachines, 11.","DOI":"10.3390\/mi11030297"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3794","DOI":"10.1039\/D3LC00320E","article-title":"Controlling amorphous silicon in scratching for fabricating high-performance micromixers","volume":"23","author":"Chen","year":"2023","journal-title":"Lab Chip"},{"key":"ref_13","first-page":"8","article-title":"Fabrication of conical microstructure array for stable droplet generation over wide flow rate range","volume":"20","author":"Chen","year":"2024","journal-title":"Small"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Orbay, S., and Sanyal, A. (2023). Molecularly imprinted polymeric particles created using droplet-based microfluidics: Preparation and applications. Micromachines, 14.","DOI":"10.3390\/mi14040763"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1063\/5.0238393","article-title":"A comprehensive review on the fundamental principles, innovative designs, and multidisciplinary applications of micromixers","volume":"36","author":"Han","year":"2024","journal-title":"Phys. Fluids"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cep.2024.109677","article-title":"Optimal design of micromixer for preparation of nanoliposomes","volume":"196","author":"Qi","year":"2024","journal-title":"Chem. Eng. Process."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6077","DOI":"10.1039\/D4RA08321K","article-title":"Microfluidic chip designs and their application for E antigen typing on red blood cells","volume":"15","author":"Maraming","year":"2025","journal-title":"RSC Adv."},{"key":"ref_18","first-page":"12","article-title":"Rapid and low-cost droplet PCR using a novel microfluidic setup with a numerical study on micromixer effects","volume":"9","author":"Pourghasemian","year":"2025","journal-title":"Sens. Actuator Rep."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wu, Y., Liu, Y., Ma, J., Zhu, S., Zhao, X., Mou, H., Ke, X., Wu, Z., Wang, Y., and Lin, S. (2025). A microfluidic biosensor for quantitative detection of salmonella in traditional chinese medicine. Biosensors, 15.","DOI":"10.3390\/bios15010010"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.heliyon.2024.e34790","article-title":"A review on biodiesel micromixers: Types of micromixers, configurations, and flow patterns","volume":"10","author":"Bizualem","year":"2024","journal-title":"Heliyon"},{"key":"ref_21","first-page":"26","article-title":"Micromixer research trend of active and passive designs","volume":"293","author":"Bazaz","year":"2024","journal-title":"Chem. Eng. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1063\/5.0179750","article-title":"Acoustohydrodynamic micromixers: Basic mixing principles, programmable mixing prospectives, and biomedical applications","volume":"18","author":"Bai","year":"2024","journal-title":"Biomicrofluidics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1687814016646264","DOI":"10.1177\/1687814016646264","article-title":"Alternating current electrothermal micromixer with thin film resistive heaters","volume":"8","author":"Zhang","year":"2016","journal-title":"Adv. Mech. Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jnnfm.2019.05.007","article-title":"Influence of varying zeta potential on non-Newtonian flow mixing in a wavy patterned microchannel","volume":"269","author":"Banerjee","year":"2019","journal-title":"J. Non-Newton. Fluid Mech."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Goodarzi, V., Hayati Jafarbeygi, S., Taheri, R.A., Sheremet, M., and Ghalambaz, M. (2021). Numerical investigation of mixing by induced electrokinetic flow in T-micromixer with conductive curved arc plate. Symmetry, 13.","DOI":"10.3390\/sym13060915"},{"key":"ref_26","first-page":"10","article-title":"A bionic microdroplet array chip for particle trapping","volume":"37","author":"Du","year":"2025","journal-title":"Phys. Fluids"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1021\/acsami.4c15647","article-title":"Evaluating the potential of microdroplet flow in two-phase biocatalysis: A systematic study","volume":"17","author":"Xiang","year":"2025","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1080\/10408347.2023.2177097","article-title":"Microfluidic fabrication and applications of microgels and hybrid microgels","volume":"54","author":"Farooqi","year":"2024","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1039\/C6LC01018K","article-title":"Passive and active droplet generation with microfluidics: A review","volume":"17","author":"Zhu","year":"2017","journal-title":"Lab Chip"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ces.2024.121103","article-title":"Fluctuation-resistant microfluidic droplet generator based on multichannel constant flow regulator","volume":"305","author":"Liao","year":"2025","journal-title":"Chem. Eng. Sci."},{"key":"ref_31","first-page":"304","article-title":"Active\/passive control and mechanism of the adhesion behaviors of droplet\/liquid-infused surface","volume":"52","author":"Zhang","year":"2023","journal-title":"Surf. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.snb.2022.132183","article-title":"PZT actuator-controlled high-frequency microdroplet generation: Reducing the restrictions of channel size, fluid viscosity, and flow rate","volume":"368","author":"Zhang","year":"2022","journal-title":"Sens. Actuator B Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bios.2021.113822","article-title":"Advances in droplet microfluidics for SERS and Raman analysis","volume":"198","author":"Yue","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6233","DOI":"10.1021\/acs.langmuir.2c00491","article-title":"Trends in droplet microfluidics: From droplet generation to biomedical applications","volume":"38","author":"Chen","year":"2022","journal-title":"Langmuir"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1039\/D1LC00836F","article-title":"Materials and methods for droplet microfluidic device fabrication","volume":"22","author":"Elvira","year":"2022","journal-title":"Lab Chip"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/ceat.202100368","article-title":"Experimental and numerical investigation of a novel spiral micromixer with Sinusoidal Channel walls","volume":"45","author":"Bahrami","year":"2022","journal-title":"Chem. Eng. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"46044","DOI":"10.1021\/acsami.4c08896","article-title":"Size-controllable and monodispersed lipid nanoparticle production with high mrna delivery efficiency using 3d-printed ring micromixers","volume":"16","author":"Hong","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1088\/1361-6439\/ad809a","article-title":"Micromixing strategies for efficient mixing processes: A comprehensive review","volume":"34","author":"Soltani","year":"2024","journal-title":"J. Micromech. Microeng."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ansari, M.A., Kim, K.Y., and Kim, S.M. (2018). Numerical and experimental study on mixing performances of simple and vortex micro T-mixers. Micromachines, 9.","DOI":"10.3390\/mi9050204"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Javaid, M.U., Cheema, T.A., and Park, C.W. (2018). Analysis of passive mixing in a serpentine microchannel with sinusoidal side walls. Micromachines, 9.","DOI":"10.3390\/mi9010008"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s10404-024-02783-3","article-title":"Performance analysis of T-shaped micromixers using an innovative bend structure of mixing channel","volume":"29","author":"Rasheed","year":"2025","journal-title":"Microfluid. Nanofluid."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cep.2025.110181","article-title":"Design and optimization of Tesla micromixer with asymmetrical arrangement for efficient mixing in microfluidic chip","volume":"209","author":"Li","year":"2025","journal-title":"Chem. Eng. Process."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1063\/5.0228640","article-title":"Effect of induced charge forming active vortex structures in serpentine microchannels on fluid mixing driven by pressure flow","volume":"36","author":"Yuan","year":"2024","journal-title":"Phys. Fluids"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cej.2020.128252","article-title":"Mixing intensification using sound-driven micromixer with sharp edges","volume":"410","author":"Zhang","year":"2021","journal-title":"Chem. Eng. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.cherd.2024.07.015","article-title":"Deformable baffles coupled with pulsatile flow improve mixing in microfluidic devices","volume":"208","author":"Jahangirifard","year":"2024","journal-title":"Chem. Eng. Res. Des."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1063\/5.0211204","article-title":"Microfluidic mixing by magnetic particles: Progress and prospects","volume":"18","author":"Misra","year":"2024","journal-title":"Biomicrofluidics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cej.2024.155348","article-title":"Mass transfer and mixing performance in jet-to-counterflow micromixer","volume":"498","author":"Ruan","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cej.2024.151183","article-title":"Chaotic-flow-driven mixing in T- and V-shaped micromixers","volume":"489","author":"Asano","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1063\/5.0178939","article-title":"Engineering advancements in microfluidic systems for enhanced mixing at low Reynolds numbers","volume":"18","author":"Gande","year":"2024","journal-title":"Biomicrofluidics"},{"key":"ref_50","first-page":"3464","article-title":"Optimizing passive micromixers: Enhancing mixing efficiency through computational fluid dynamics and metaheuristic algorithms","volume":"18","author":"Mustafa","year":"2024","journal-title":"Can. J. Chem. Eng."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Zoupanou, S., Chiriac\u00f2, M.S., Tarantini, I., and Ferrara, F. (2021). Innovative 3D microfluidic tools for on-chip fluids and particles manipulation: From design to experimental validation. Micromachines, 12.","DOI":"10.3390\/mi12020104"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Qin, L., Jiang, Z., Zhou, D., Yue, J., and Cheng, H. (2025). Numerical investigation of vortex-induced enhancement in the mixing characteristics of double-spiral and serpentine microchannels. Micromachines, 16.","DOI":"10.3390\/mi16091016"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Chen, Z., Wang, Y., and Zhou, S. (2022). Numerical analysis of mixing performance in an electroosmotic micromixer with cosine channel walls. Micromachines, 13.","DOI":"10.3390\/mi13111933"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Mahammedi, A., Kouider, R., Tayeb, N.T., Al-Karany, R.K., Cuerda-Correa, E.M., and Al-Kassir, A. (2024). Thermal and hydrodynamic measurements of a novel chaotic micromixer to enhance mixing performance. Energies, 17.","DOI":"10.3390\/en17133248"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.compfluid.2014.09.024","article-title":"Mixing enhancement in a passive micromixer with convergent-divergent sinusoidal microchannels and different ratio of amplitude to wave length","volume":"105","author":"Parsa","year":"2014","journal-title":"Comput. Fluids"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.cep.2018.12.011","article-title":"Numerical study of mixing in wavy micromixers: Comparison between raccoon and serpentine mixer","volume":"136","author":"Mondal","year":"2019","journal-title":"Chem. Eng. Process."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107850","DOI":"10.1016\/j.icheatmasstransfer.2024.107850","article-title":"Non-Newtonian fluid mixing in spiral micromixers: An extensive numerical analysis","volume":"158","author":"Kharaji","year":"2024","journal-title":"Int. Commun. Heat Mass Transf."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.cep.2022.109246","article-title":"Design and analysis of a novel Bi-layer curved serpentine chaotic micromixer for efficient mixing","volume":"183","author":"Raza","year":"2023","journal-title":"Chem. Eng. Process."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.cep.2021.108756","article-title":"Analysis of different shapes of cross-sections and obstacles in variable-radius spiral micromixers on mixing efficiency","volume":"171","author":"Jiang","year":"2022","journal-title":"Chem. Eng. Process."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1007\/s11012-023-01676-z","article-title":"Comparative assessments of mixing and pressure drop characteristics in spiral, serpentine and straight micromixers","volume":"58","author":"Tripathi","year":"2023","journal-title":"Meccanica"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"034120","DOI":"10.1063\/1.4954812","article-title":"A \u201ctwisted\u201d microfluidic mixer suitable for a wide range of flow rate applications","volume":"10","author":"Sivashankar","year":"2016","journal-title":"Biomicrofluidics"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4135","DOI":"10.1021\/acs.iecr.2c04131","article-title":"Mixing enhancement of newtonian liquids in a curvature induced split and recombine micromixer","volume":"16","author":"Ghosh","year":"2023","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1007\/s11696-024-03380-y","article-title":"Optimization of a novel micromixer with fan-shaped obstacles","volume":"78","author":"Ghahfarokhi","year":"2024","journal-title":"Chem. Pap."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e14745","DOI":"10.1016\/j.heliyon.2023.e14745","article-title":"Modeling and simulation of a split and recombination-based passive micromixer with vortex-generating mixing units","volume":"9","author":"Nishu","year":"2023","journal-title":"Heliyon"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.ijheatmasstransfer.2019.05.084","article-title":"Fluid micro-mixing in a passive microchannel: Comparison of 2D and 3D numerical simulations","volume":"139","author":"Haghighinia","year":"2019","journal-title":"Int. J. Heat Mass Transf."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Yuan, B., Yuan, S., and Wang, H. (2026). Numerical and experimental investigation of mixing enhancement in a zigzag passive micromixer with D-shaped obstacles. Micromachines, 17.","DOI":"10.3390\/mi17020190"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"4474","DOI":"10.1039\/D0LC00823K","article-title":"Monolithic 3D micromixer with an impeller for glass microfluidic systems","volume":"20","author":"Kim","year":"2020","journal-title":"Lab Chip"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Chen, L., Deng, Y., Zhou, T., Pan, H., and Liu, Z. (2017). A novel electroosmotic micromixer with asymmetric lateral structures and DC electrode arrays. Micromachines, 8.","DOI":"10.3390\/mi8040105"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Raza, W., Ma, S.B., and Kim, K.Y. (2019). Single and multi-objective optimization of a three-dimensional unbalanced split-and-recombine micromixer. Micromachines, 10.","DOI":"10.3390\/mi10100711"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"8850","DOI":"10.1021\/acs.analchem.3c00335","article-title":"A directly moldable, highly compact, and easy-for-integration 3D micromixer with extraordinary mixing performance","volume":"95","author":"Wang","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"075007","DOI":"10.1088\/1361-6439\/ac7771","article-title":"Performance evaluation of a 3D split-and-recombination micromixer with asymmetric structures","volume":"32","author":"Jiao","year":"2022","journal-title":"J. Micromech. Microeng."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1007\/s11814-024-00161-x","article-title":"Comparison and analysis of mixing efficiency in various micromixer designs","volume":"41","author":"Oh","year":"2024","journal-title":"Korean J. Chem. Eng."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Salieb-Beugelaar, G.B., Gon\u00e7alves, D., Wolf, M.P., and Hunziker, P. (2016). Microfluidic 3D helix mixers. Micromachines, 7.","DOI":"10.3390\/mi7100189"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/j.snb.2016.08.116","article-title":"Effect of channel aspect ratio of 3-D T-mixer on flow patterns and convective mixing for a wide range of Reynolds number","volume":"239","author":"Azarbadegan","year":"2017","journal-title":"Sens. Actuator B Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1039\/D0LC01120G","article-title":"Nonlinear microfluidics: Device physics, functions, and applications","volume":"21","author":"Xia","year":"2021","journal-title":"Lab Chip"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1039\/c2lc90046g","article-title":"Microfluidic synthesis of multifunctional Janus particles for biomedical applications","volume":"12","author":"Yang","year":"2012","journal-title":"Lab Chip"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"6649","DOI":"10.1002\/adma.201601504","article-title":"Simple spinning of heterogeneous hollow microfibers on chip","volume":"28","author":"Yu","year":"2016","journal-title":"Adv. Mater."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1073\/pnas.1617260114","article-title":"Flow-assisted assembly of nanostructured protein microfibers","volume":"114","author":"Kamada","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/smll.201903940","article-title":"Microfluidics for biosynthesizing: From droplets and vesicles to artificial cells","volume":"16","author":"Ai","year":"2020","journal-title":"Small"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/nphys1513","article-title":"Static control logic for microfluidic devices using pressure-gain valves","volume":"6","author":"Weaver","year":"2010","journal-title":"Nat. Phys."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.1002\/smll.201200456","article-title":"Microfluidic automation using elastomeric valves and droplets: Reducing reliance on external controllers","volume":"8","author":"Kim","year":"2012","journal-title":"Small"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/s41586-019-1701-6","article-title":"Braess\u2019s paradox and programmable behaviour in microfluidic networks","volume":"574","author":"Case","year":"2020","journal-title":"Nature"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"128503","DOI":"10.1016\/j.snb.2020.128503","article-title":"Numerical investigation of the secondary flow effect of lateral structure of micromixing channel on laminar flow","volume":"321","author":"Shi","year":"2020","journal-title":"Sens. Actuator B Chem."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Ding, L., Razavi Bazaz, S., Shrestha, J.A., Amiri, H., Mas-Hafi, S., Banerjee, B., Vesey, G., Miansari, M., and Ebrahimi Warkiani, M. (2022). Rapid and continuous cryopreservation of stem cells with a 3D micromixer. Micromachines, 13.","DOI":"10.3390\/mi13091516"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1002\/elps.202200077","article-title":"A novel 3D Tesla valve micromixer for efficient mixing and chitosan nanoparticle production","volume":"43","author":"Guo","year":"2022","journal-title":"Electrophoresis"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cej.2023.146481","article-title":"A 3D homogeneous microreactor with high mixing intensity at wide Re range for MOF preparation and POCT application","volume":"476","author":"Tan","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Mehrdel, P., Karimi, S., Farr\u00e9-Llad\u00f3s, J., and Casals-Terr\u00e9, J. (2018). Novel variable radius spiral-shaped micromixer: From numerical analysis to experimental validation. Micromachines, 9.","DOI":"10.3390\/mi9110552"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jwpe.2024.105163","article-title":"Enhanced fluid mixing using a novel multi-stage static pipeline mixer: Numerical simulation and mechanism analysis","volume":"60","author":"Zhao","year":"2024","journal-title":"J. Water Process. Eng."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Fu, Q., Liu, Z.H., Cao, S.Q., Wang, Z., and Liu, G.J. (2023). Topology-optimized micromixer design with enhanced reverse flow to increase mixing efficiency. Micromachines, 14.","DOI":"10.3390\/mi14081599"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.cherd.2024.12.037","article-title":"Design, evaluation, and optimization of a new micromixer based on three-dimensional multilaminar flow","volume":"214","author":"Chen","year":"2025","journal-title":"Chem. Eng. Res. Des."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1039\/D3LC00647F","article-title":"Artificial intelligence-accelerated high-throughput screening of antibiotic combinations on a microfluidic combinatorial droplet system","volume":"23","author":"Yang","year":"2023","journal-title":"Lab Chip"},{"key":"ref_92","first-page":"10","article-title":"Polymer capsules with tunable shell thickness synthesized via janus-to-core shell transition of biphasic droplets produced in a microfluidic flow-focusing device","volume":"10","author":"Xu","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_93","first-page":"7","article-title":"Experimental investigation of non-Newtonian droplet splitting mechanism in a cross-flow type microfluidic device","volume":"48","author":"Wankawala","year":"2023","journal-title":"Sadhana-Acad. Proc. Eng. Sci."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.colsurfa.2022.128536","article-title":"Interface evolution and pinch-off mechanism of droplet in two-phase liquid flow through T-junction microfluidic system","volume":"642","author":"Venkateshwarlu","year":"2022","journal-title":"Colloid Surf. A Physicochem. Eng. Asp."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Huang, B., Xie, H.Y., and Li, Z.Z. (2023). Microfluidic methods for generation of submicron droplets: A review. Micromachines, 14.","DOI":"10.3390\/mi14030638"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1039\/C5SM02051D","article-title":"Controlling and predicting droplet size of nanoemulsions: Scaling relations with experimental validation","volume":"12","author":"Gupta","year":"2016","journal-title":"Soft Matter"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.colsurfa.2019.02.040","article-title":"Investigation of microfluidic co-flow effects on step emulsification: Interfacial tension and flow velocities","volume":"568","author":"Lian","year":"2019","journal-title":"Colloid Surf. A Physicochem. Eng. Asp."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Venkateshwarlu, A., and Bharti, R.P. (2022). Computational analysis of interface evolution and droplet pinch-off mechanism in two-phase liquid flow through T-junction microfluidic system. arXiv.","DOI":"10.1016\/j.colsurfa.2022.128536"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41467-019-10505-5","article-title":"Accounting for corner flow unifies the understanding of droplet formation in microfluidic channels","volume":"10","author":"Korczyk","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Stevens, M., Balaur, E., and Abbey, B. (2023). Computational simulation of the effects of interfacial tension in microfluidic flow focusing droplet generators. Front. Phys., 11.","DOI":"10.3389\/fphy.2023.1060780"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"051304","DOI":"10.1063\/5.0267868","article-title":"Droplet splitting in microfluidics: A review","volume":"37","author":"Agnihotri","year":"2025","journal-title":"Phys. Fluids"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cis.2020.102208","article-title":"Multiphase flow in microfluidics: From droplets and bubbles to the encapsulated structures","volume":"282","author":"Sattari","year":"2020","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Cho, Y., Kim, J., Park, J., Kim, H.S., and Cho, Y. (2023). Monodisperse micro-droplet generation in microfluidic channel with asymmetric cross-sectional shape. Micromachines, 14.","DOI":"10.3390\/mi14010223"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ces.2022.117947","article-title":"A microfluidic droplet system for ultra-monodisperse droplet generation: A universal approach","volume":"261","author":"Kalantarifard","year":"2022","journal-title":"Chem. Eng. Sci."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s41467-020-20284-z","article-title":"Machine learning enables design automation of microfluidic flow-focusing droplet generation","volume":"12","author":"Lashkaripour","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"344971","DOI":"10.1016\/j.aca.2025.344971","article-title":"Development of 3D printed microchips with encapsulated channels as miniaturized separation devices for bioanalysis","volume":"1384","author":"Desire","year":"2026","journal-title":"Anal. Chim. Acta"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/s43586-023-00212-3","article-title":"Droplet-based microfluidics","volume":"3","author":"Morneau","year":"2023","journal-title":"Nat. Rev. Methods Primers"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1039\/D5LC01055A","article-title":"A microfluidic method for controlled generation and trapping of membraneless water-in-water droplets","volume":"26","author":"Li","year":"2026","journal-title":"Lab Chip"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/aic.16777","article-title":"Mechanism of bubble formation in step-emulsification devices","volume":"66","author":"Mi","year":"2020","journal-title":"AIChE J."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"9056","DOI":"10.1039\/D0CC03628E","article-title":"Step emulsification in microfluidic droplet generation: Mechanisms and structures","volume":"56","author":"Shi","year":"2020","journal-title":"Chem. Commun."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.cherd.2019.02.024","article-title":"Characterizing droplet breakup rates of shear-thinning dispersed phase in microreactors","volume":"144","author":"Wong","year":"2019","journal-title":"Chem. Eng. Res. Des."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jmmm.2024.172645","article-title":"Droplet generation in a co-flowing microchannel under the non-uniform magnetic fields produced by an electric current loop","volume":"612","author":"Fadaei","year":"2024","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s10404-025-02796-6","article-title":"Advancements in microfluidic droplet generation: Methods and insights","volume":"29","author":"Das","year":"2025","journal-title":"Microfluid. Nanofluid."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/apj.2997","article-title":"Numerical study on active droplet generation governed by pulsatile continuous-phase flow","volume":"19","author":"Zhang","year":"2024","journal-title":"Asia-Pac. J. Chem. Eng."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1063\/5.0261531","article-title":"Droplet acoustofluidics: Recent progress and challenges","volume":"19","author":"Ali","year":"2025","journal-title":"Biomicrofluidics"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1039\/D1LC00801C","article-title":"Manipulation of cancer cells in a sessile droplet via travelling surface acoustic waves","volume":"22","author":"Nam","year":"2021","journal-title":"Lab Chip"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Lei, Y.L., Hu, H., Chen, J., and Zhang, P. (2020). Microfluidic Jetting Deformation and Pinching-off Mechanism in Capillary Tubes by Using Traveling Surface Acoustic Waves. Actuators, 9.","DOI":"10.3390\/act9010005"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1039\/C5LC00217F","article-title":"Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures","volume":"15","author":"Moon","year":"2015","journal-title":"Lab Chip"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"3922","DOI":"10.1039\/D0LC00803F","article-title":"Acoustofluidic generation of droplets with tunable chemical concentrations","volume":"20","author":"Park","year":"2020","journal-title":"Lab Chip"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"095310","DOI":"10.1063\/5.0107610","article-title":"Experimental investigation on the performances of a valve-based and on-demand droplet generator producing droplets in a wide size range","volume":"12","author":"Wang","year":"2022","journal-title":"AIP Adv."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"0856","DOI":"10.34133\/research.0856","article-title":"Innovative advances in droplet microfluidics","volume":"8","author":"Zhang","year":"2025","journal-title":"Research"},{"key":"ref_122","unstructured":"Cho, S. (2024). Manipulation of Droplet-Based Microfluidic Systems: Artificial Intelligence, Electrohydrodynamics Approaches, and Engineering Applications. [Doctoral Dissertation, Sungkyunkwan University (SKKU)]."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"2409341","DOI":"10.1002\/adfm.202409341","article-title":"Electrocapillarity-induced hurricane-in-a-tube enables the generation and patterning of liquid metal droplets","volume":"34","author":"Tao","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"12580","DOI":"10.1021\/acs.langmuir.4c00403","article-title":"Drop-on-demand microdroplet generation under charge injection by corona discharge","volume":"40","author":"Tang","year":"2024","journal-title":"Langmuir"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"2500074","DOI":"10.1002\/admi.202500074","article-title":"Effective manipulation of water droplets on open superhydrophobic glass surfaces by using a triboelectrically charged polytetrafluoroethylene rod on the back side of these surfaces","volume":"12","author":"Huang","year":"2025","journal-title":"Adv. Mater. Interfaces"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"1905449","DOI":"10.1002\/adma.201905449","article-title":"Controllable High-Speed Electrostatic Manipulation of Water Droplets on a Superhydrophobic Surface","volume":"31","author":"Dai","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.cjac.2024.100439","article-title":"Numerical simulation of droplet formation in a Co-flow microchannel capillary device","volume":"52","author":"Zhang","year":"2024","journal-title":"Chin. J. Anal. Chem."},{"key":"ref_128","first-page":"1291","article-title":"Numerical Investigation of the Influence of Microchannel Geometry on the Droplet Generation Process","volume":"15","author":"Paramnantham","year":"2022","journal-title":"J. Appl. Fluid Mech."},{"key":"ref_129","first-page":"1","article-title":"Research progress of droplet generation and detection technology of microfluidic chip","volume":"38","author":"Zheng","year":"2021","journal-title":"Chin. J. Appl. Chem."},{"key":"ref_130","first-page":"12","article-title":"Effect of surfactants on droplet generation in a microfluidic T-junction: A lattice Boltzmann study","volume":"34","author":"Zhang","year":"2022","journal-title":"Phys. Fluids"},{"key":"ref_131","first-page":"487","article-title":"Numerical simulation of droplet generation in coaxial microchannels","volume":"20","author":"Yin","year":"2024","journal-title":"Fluid Dyn. Mater. Process."},{"key":"ref_132","first-page":"566","article-title":"Experimental investigation of droplet formation in coaxial microchannels with different geometries of inner channel","volume":"75","author":"Song","year":"2024","journal-title":"CIESC J."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.snb.2024.136617","article-title":"Flexible droplet microfluidic devices for tuneable droplet generation","volume":"422","author":"Roshan","year":"2025","journal-title":"Sens. Actuator B Chem."},{"key":"ref_134","first-page":"14","article-title":"Generation of monodisperse micro-droplets within the stable narrowing jetting regime: Effects of viscosity and interfacial tension","volume":"26","author":"Zhang","year":"2022","journal-title":"Microfluid. Nanofluid."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/micronano.2016.91","article-title":"Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition","volume":"3","author":"Trantidou","year":"2017","journal-title":"Microsyst. Nanoeng."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/smll.202412099","article-title":"Artificial intelligence-empowered automated double emulsion droplet library generation","volume":"21","author":"Shin","year":"2025","journal-title":"Small"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1038\/s41467-025-58139-0","article-title":"AI-driven high-throughput droplet screening of cell-free gene expression","volume":"16","author":"Zhu","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_138","doi-asserted-by":"crossref","unstructured":"Xiao, J.F., Chen, Z.Y., Gao, Y., and Wu, X.B. (2019, January 8\u201311). The Doppler Frequency of Moving Object by FDTD. Proceedings of the International Applied-Computational-Electromagnetics-Society Symposium\u2014China (ACES), Nanjing, China.","DOI":"10.23919\/ACES48530.2019.9060791"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.flowmeasinst.2016.08.005","article-title":"A new visual tracking method for the analysis and characterization of jet flow","volume":"51","author":"Alcan","year":"2016","journal-title":"Flow Meas. Instrum."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ces.2020.116093","article-title":"Theoretical and experimental limits of monodisperse droplet generation","volume":"229","author":"Kalantarifard","year":"2021","journal-title":"Chem. Eng. Sci."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1063\/1.4963666","article-title":"An automated system for high-throughput generation and optimization of microdroplets","volume":"10","author":"Wang","year":"2016","journal-title":"Biomicrofluidics"},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Isa, N.S.M., El Kadri, H., Vigolo, D., and Gkatzionis, K. (2022). The effect of bacteria on the stability of microfluidic-generated water-in-oil droplet. Micromachines, 13.","DOI":"10.3390\/mi13122067"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.1088\/0951-7715\/28\/9\/3175","article-title":"Equilibria and their stability for a viscous droplet model","volume":"28","author":"Guidotti","year":"2015","journal-title":"Nonlinearity"},{"key":"ref_144","first-page":"11","article-title":"Comparative experimental and numerical study of mixing efficiency in 3D-printed microfluidic droplet generators: T junction, cross junction, and asymmetric junctions with varying angles","volume":"205","author":"Barzoki","year":"2024","journal-title":"Chem. Eng. Process."},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Chung, C.H.Y., Cui, B., Song, R., Liu, X., Xu, X., and Yao, S. (2019). Scalable production of monodisperse functional microspheres by multilayer parallelization of high aspect ratio microfluidic channels. Micromachines, 10.","DOI":"10.3390\/mi10090592"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41378-026-01175-7","article-title":"Droplet microfluidics for biomedical applications: Emerging trends and future developments","volume":"12","author":"Ma","year":"2026","journal-title":"Microsyst. Nanoeng."},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"Shen, C., Liu, F., Wu, L., Yu, C., and Yu, W. (2020). Dripping, jetting and regime transition of droplet formation in a buoyancy-assisted microfluidic device. Micromachines, 11.","DOI":"10.3390\/mi11110962"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"101262","DOI":"10.1016\/j.coche.2026.101262","article-title":"Droplet microfluidics for single-microorganism detection and analysis","volume":"52","author":"Nan","year":"2026","journal-title":"Curr. Opin. Chem. Eng."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s00414-024-03306-z","article-title":"Discovery unbalanced DNA mixtures and evaluation mixing ratio via a droplet digital PCR method","volume":"139","author":"Fu","year":"2025","journal-title":"Int. J. Leg. Med."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1002\/smtd.202300730","article-title":"Creation of a high-throughput microfluidic platform for single-cell transcriptome sequencing of cell-cell interactions","volume":"7","author":"Qi","year":"2023","journal-title":"Small Methods"},{"key":"ref_151","doi-asserted-by":"crossref","unstructured":"Tony, A., Badea, I., Yang, C., Liu, Y., Wells, G., Wang, K., Yin, R., Zhang, H., and Zhang, W. (2023). The additive manufacturing approach to polydimethylsiloxane (pdms) microfluidic devices: Review and future directions. Polymers, 15.","DOI":"10.3390\/polym15081926"},{"key":"ref_152","doi-asserted-by":"crossref","unstructured":"Tony, A., Badea, I., Yang, C., Liu, Y., Wang, K., Yang, S.M., and Zhang, W. (2023). A preliminary experimental study of polydimethylsiloxane (PDMS)-to-PDMS bonding using oxygen plasma treatment incorporating isopropyl alcohol. Polymers, 15.","DOI":"10.3390\/polym15041006"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1063\/5.0122055","article-title":"A simple and accessible approach for processing photopolymer master molds for the fabrication of microfluidic polydimethylsiloxane devices","volume":"34","author":"Otroshchenko","year":"2022","journal-title":"Phys. Fluids"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s10404-023-02698-5","article-title":"Superhydrophobic treatment of PDMS-based microfluidic devices using CO2 laser ablation","volume":"28","author":"Yajun","year":"2024","journal-title":"Microfluid. Nanofluid."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"4296","DOI":"10.1039\/C6LC01058J","article-title":"Unconventional micro-\/nanofabrication technologies for hybrid-scale lab-on-a-chip","volume":"16","author":"Ha","year":"2016","journal-title":"Lab Chip"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s42242-020-00112-5","article-title":"3D printed microfluidic devices: A review focused on four fundamental manufacturing approaches and implications on the field of healthcare","volume":"4","author":"Mehta","year":"2021","journal-title":"Bio-Des. Manuf."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.jiec.2021.03.033","article-title":"CO2 laser machining for microfluidics mold fabrication from PMMA with applications on viscoelastic focusing, electrospun nanofiber production, and droplet generation","volume":"98","author":"Guler","year":"2021","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1108\/RPJ-06-2024-0236","article-title":"3D printed microfluidic devices produced by material extrusion: Effect of ironing parameters on mixing efficiency and gradient generation","volume":"31","author":"Pricci","year":"2025","journal-title":"Rapid Prototyp. J."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1039\/D4LC00009A","article-title":"Versatile hybrid technique for passive straight micromixer manufacturing by combining pulsed laser ablation, stereolithographic 3D printing and computational fluid dynamics","volume":"24","author":"Carnero","year":"2024","journal-title":"Lab Chip"},{"key":"ref_160","doi-asserted-by":"crossref","unstructured":"Sasaki, N., and Sugenami, E. (2021). Fabrication of a t-shaped microfluidic channel using a consumer laser cutter and application to monodisperse microdroplet formation. Micromachines, 12.","DOI":"10.3390\/mi12020160"},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1039\/D2LC01177H","article-title":"3D printed microfluidics: Advances in strategies, integration, and applications","volume":"23","author":"Su","year":"2023","journal-title":"Lab Chip"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.snb.2022.132642","article-title":"Template-free lithography for cross-scale channels towards enhancing nanofluidic devices","volume":"372","author":"Wu","year":"2022","journal-title":"Sens. Actuator B Chem."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jcis.2019.01.119","article-title":"Versatile reconfigurable glass capillary microfluidic devices with Lego inspired blocks for drop generation and micromixing","volume":"542","author":"Bandulasena","year":"2019","journal-title":"J. Colloid Interface Sci."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"18230","DOI":"10.1021\/acs.langmuir.4c02316","article-title":"Concentric capillary microfluidic devices designed for robust production of multiple-emulsion droplets","volume":"40","author":"Oh","year":"2024","journal-title":"Langmuir"},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1246\/cl.210144","article-title":"Glass capillary microfluidic device utilizing pipette tips for droplet formation","volume":"50","author":"Komazaki","year":"2021","journal-title":"Chem. Lett."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1039\/D3RA08561A","article-title":"Effective colloidal emulsion droplet regulation in flow-focusing glass capillary microfluidic device via collection tube variation","volume":"14","author":"Jiang","year":"2024","journal-title":"RSC Adv."},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"4410","DOI":"10.1039\/D5LC00415B","article-title":"Recovery of phenotypically sorted cells using droplet-digital microfluidics","volume":"25","author":"Deng","year":"2025","journal-title":"Lab Chip"},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.trac.2023.116932","article-title":"Recent advances in droplet microfluidics for single-cell analysis","volume":"159","author":"Jiang","year":"2023","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1088\/0960-1317\/17\/4\/011","article-title":"Surface microfluidics-high-speed DEP liquid actuation on planar substrates and critical factors in reliable","volume":"17","author":"Kanagasabapathi","year":"2007","journal-title":"J. Micromech. Microeng."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1039\/D2LC00756H","article-title":"Digital microfluidics for biological analysis and applications","volume":"23","author":"Xu","year":"2023","journal-title":"Lab Chip"},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41598-021-88174-y","article-title":"An asymmetric flow-focusing droplet generator promotes rapid mixing of reagents","volume":"11","author":"Belousov","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_172","doi-asserted-by":"crossref","unstructured":"Huang, C., Jiang, Y.Q., Li, Y.W., and Zhang, H. (2023). Droplet detection and sorting system in microfluidics: A review. Micromachines, 14.","DOI":"10.3390\/mi14010103"},{"key":"ref_173","first-page":"17","article-title":"Microfluidic single-cell omics analysis","volume":"16","author":"Xu","year":"2020","journal-title":"Small"},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/adma.202406333","article-title":"Strong elastic protein nanosheets enable the culture and differentiation of induced pluripotent stem cells on microdroplets","volume":"36","author":"Mojares","year":"2024","journal-title":"Adv. Mater."},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"2294","DOI":"10.1039\/D1AN02321G","article-title":"Single-cell droplet microfluidics for biomedical applications","volume":"147","author":"Liu","year":"2022","journal-title":"Analyst"},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1007\/s13206-016-0407-1","article-title":"Microdroplet-based cell culture models and their application","volume":"10","author":"Jang","year":"2016","journal-title":"BioChip J."},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"8544","DOI":"10.1021\/acs.langmuir.1c01005","article-title":"Parametric Study of the factors influencing liposome physicochemical characteristics in a periodic disturbance mixer","volume":"37","author":"Ocampo","year":"2021","journal-title":"Langmuir"},{"key":"ref_178","doi-asserted-by":"crossref","unstructured":"Damiati, S., Kompella, U.B., Damiati, S.A., and Kodzius, R. (2018). Microfluidic devices for drug delivery systems and drug screening. Genes, 9.","DOI":"10.3390\/genes9020103"},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1039\/C8LC01178H","article-title":"Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing","volume":"19","author":"Babahosseini","year":"2019","journal-title":"Lab Chip"},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"6544","DOI":"10.1021\/ac0346712","article-title":"Solvent compatibility of poly(dimethylsiloxane)-based microfluidic devices","volume":"75","author":"Lee","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"8085","DOI":"10.1021\/acs.analchem.2c00615","article-title":"Droplet microfluidics for high-resolution virology","volume":"94","author":"Jing","year":"2022","journal-title":"Anal. Chem."},{"key":"ref_182","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1039\/D1LC01097B","article-title":"Microfluidics and surface-enhanced Raman spectroscopy, a win-win combination?","volume":"22","author":"Panneerselvam","year":"2022","journal-title":"Lab Chip"},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfb.2016.07.041","article-title":"Enhancing the biocompatibility of microfluidics-assisted fabrication of cell-laden microgels with channel geometry","volume":"147","author":"Kim","year":"2016","journal-title":"Colloid Surf. B Biointerfaces"},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.actbio.2020.05.024","article-title":"Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration","volume":"111","author":"An","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1002\/term.2818","article-title":"Strategies for controlling egress of therapeutic cells from hydrogel microcapsules","volume":"13","author":"Haase","year":"2019","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.21037\/tlcr-20-1010","article-title":"Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR","volume":"10","author":"Silveira","year":"2021","journal-title":"Transl. Lung Cancer Res."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00604-023-05827-7","article-title":"Hydrogel capsule-based digital quantitative polymerase chain reaction","volume":"190","author":"Tan","year":"2023","journal-title":"Microchim. Acta"}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/17\/8\/963\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:16:00Z","timestamp":1787055360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/17\/8\/963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,15]]},"references-count":187,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["mi17080963"],"URL":"https:\/\/doi.org\/10.3390\/mi17080963","relation":{},"ISSN":["2072-666X"],"issn-type":[{"value":"2072-666X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,15]]}}}