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Considerable efforts have been directed toward developing plant-on-chip platforms to investigate cellular responses to external stimuli, including chemical, mechanical, and electrical cues. In this study, we present a fluidic platform using polydimethylsiloxane (PDMS) and a printed circuit board (PCB), integrated with electrochemical impedance spectroscopy (EIS) detection. Various experimental conditions were examined, including ionic and pH stimulation, as well as membrane dimensions, with the onion inner membrane treated as a black-box system. The measurement results are presented as Nyquist plots, and a resistance model incorporating multifactorial influences is proposed. Impedance variations in plant cells serve as a basis for electrical modulation. To explore these properties, we converted acoustic signals into electrical inputs and recorded the outputs after being modulated by onion inner epidermal cells. A transfer function analysis was subsequently performed. Our results indicate that the plant cell-on-chip (PCOC) platform holds promise for further investigations into plant cell properties. The impedance results suggest that plant cells can respond to different external stimuli, enabling modulation of the electrical properties. These findings lay the groundwork for future studies on cellular electrical characteristics and the development of preliminary bioelectrical circuits.<\/jats:p>","DOI":"10.3390\/mi17091015","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T12:03:43Z","timestamp":1787832223000},"page":"1015","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plant Cell-on-Chip (PCOC): Exploring the Electrical Modulation Capability of Plant Cells"],"prefix":"10.3390","volume":"17","author":[{"given":"Jiayu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Microelectronics, Tianjin University, Tianjin 300072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3586-0935","authenticated-orcid":false,"given":"Ruyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, Notre Dame, IN 46556, USA"},{"name":"Department of Computer Science & Statistics, University of Rhode Island, Kingston, RI 02881, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Microelectronics, Tianjin University, Tianjin 300072, China"},{"name":"Tianjin Key Laboratory of Imaging and Sensing Microelectronic Technology, Tianjin University, Tianjin 300072, China"},{"name":"State Key Laboratory of Advanced Materials for Intelligent Sensing, Tianjin University, Tianjin 300072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical School, Tianjin University, Tianjin 300072, China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices, Tianjin 300072, China"},{"name":"School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewei","family":"Liu","sequence":"additional","affiliation":[{"name":"The Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Stedical Scientific, Carlsbad, CA 92010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8882-6362","authenticated-orcid":false,"given":"Xiang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Microelectronics, Tianjin University, Tianjin 300072, China"},{"name":"Key Laboratory of Organic Integrated Circuit, Ministry of Education, Tianjin University, Tianjin 300072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00425-023-04261-6","article-title":"Deciphering the role of mechanosensitive channels in plant root biology: Perception, signaling, and adaptive responses","volume":"258","author":"Tyagi","year":"2023","journal-title":"Planta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1093\/plcell\/koab230","article-title":"Plant cell mechanobiology: Greater than the sum of its parts","volume":"34","author":"Codjoe","year":"2022","journal-title":"Plant Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e20596","DOI":"10.7717\/peerj.20596","article-title":"Electrical impedance spectroscopy in plant cold resistance: A review","volume":"14","author":"Wang","year":"2026","journal-title":"PeerJ"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/plphys\/kiad387","article-title":"Forces on and in the cell walls of living plants","volume":"194","author":"Jarvis","year":"2023","journal-title":"Plant Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e1600035","DOI":"10.1002\/adbi.201600035","article-title":"Muscle-Cell-Based \u201cLiving Diodes\u201d","volume":"1","author":"Can","year":"2017","journal-title":"Adv. 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