{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:54:35Z","timestamp":1790517275746,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T00:00:00Z","timestamp":1640476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Despite the advances in surgical techniques and perioperative care, the complication rates after colorectal cancer surgery have remained stable. Recently, it has been suggested that colon microbiota may be implicated in several pathways that can lead to impaired colonic homeostasis and, thereby, to the development of complications after colorectal surgery. The aim of this study was to evaluate the potential impact of colonic dysbiosis on postoperative course. This prospective human clinical study recruited patients operated on for left colon, sigmoid colon or rectal cancer. Colon mucosa and fecal samples were collected to study mucosa associated microbiota (MAM) and luminal microbiota (LM), accordingly. Preliminary analysis for the first 25 consecutive patients with V3\u2013V4 16S rRNA metagenomic analysis was performed. Bacterial composition and abundance in patients who developed postoperative complications over a 90-day follow-up period were compared to those without postoperative complications. Abundance and distribution of genera in MAM differed significantly when compared to LM with a significant impact on neoadjuvant therapy on bacterial composition. Preliminary analysis revealed no statistically significant differences in LM nor in MAM composition when individuals with and without postoperative surgical complications were compared. In cases of postoperative complications, LM and MAM showed significantly decreased diversity. Composition of the colonic microbiota is altered by neoadjuvant therapy. Results on the impact of colonic dysbiosis on postoperative complications are pending the end of the present study, with 50 patients enrolled.<\/jats:p>","DOI":"10.3390\/microorganisms10010041","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T01:06:54Z","timestamp":1640567214000},"page":"41","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Human Colonic Microbiota and Short-Term Postoperative Outcomes in Colorectal Cancer Patients: A Pilot Study"],"prefix":"10.3390","volume":"10","author":[{"given":"Lelde","family":"Lauka","sequence":"first","affiliation":[{"name":"Department of Digestive and Hepatobiliary Surgery, APHP-Henri Mondor University Hospital, 94000 Creteil, France"},{"name":"EC2M-EA7375 Research Team, Henri Modor Campus, Paris East University, 94000 Creteil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5113-0660","authenticated-orcid":false,"given":"Iradj","family":"Sobhani","sequence":"additional","affiliation":[{"name":"EC2M-EA7375 Research Team, Henri Modor Campus, Paris East University, 94000 Creteil, France"},{"name":"Department of Gastroenterology, APHP-Henri Mondor University Hospital, 94000 Creteil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Department of Digestive and Hepatobiliary Surgery, APHP-Henri Mondor University Hospital, 94000 Creteil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6327-7857","authenticated-orcid":false,"given":"Denis","family":"Mestivier","sequence":"additional","affiliation":[{"name":"EC2M-EA7375 Research Team, Henri Modor Campus, Paris East University, 94000 Creteil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1211-4916","authenticated-orcid":false,"given":"Nicola","family":"de\u2019Angelis","sequence":"additional","affiliation":[{"name":"Department of Digestive and Hepatobiliary Surgery, APHP-Henri Mondor University Hospital, 94000 Creteil, France"},{"name":"EC2M-EA7375 Research Team, Henri Modor Campus, Paris East University, 94000 Creteil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA A Cancer J. 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