{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:19:18Z","timestamp":1790417958134,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Towards chlorinated solvents, the effectiveness of the remediation strategy can be improved by combining a biological approach (e.g., anaerobic reductive dechlorination) with chemical\/physical treatments (e.g., adsorption). A coupled adsorption and biodegradation (CAB) process for trichloroethylene (TCE) removal is proposed in a biofilm\u2013biochar reactor (BBR) to assess whether biochar from pine wood (PWB) can support a dechlorinating biofilm by combining the TCE (100 \u00b5M) adsorption. The BBR operated for eight months in parallel with a biofilm reactor (BR)\u2014no PWB (biological process alone), and with an abiotic biochar reactor (ABR)\u2014no dechlorinating biofilm (only an adsorption mechanism). Two flow rates were investigated. Compared to the BR, which resulted in a TCE removal of 86.9 \u00b1 11.9% and 78.73 \u00b1 19.79%, the BBR demonstrated that PWB effectively adsorbs TCE and slows down the release of its intermediates. The elimination of TCE was quantitative, with 99.61 \u00b1 0.79% and 99.87 \u00b1 0.51% TCE removal. Interestingly, the biomarker of the reductive dechlorination process, Dehalococcoides mccartyi, was found in the BRR (9.2 \u00d7 105 16S rRNA gene copies\/g), together with the specific genes tceA, bvcA, and vcrA (8.16 \u00d7 106, 1.28 \u00d7 105, and 8.01 \u00d7 103 gene copies\/g, respectively). This study suggests the feasibility of biochar to support the reductive dechlorination of D. mccartyi, opening new frontiers for field-scale applications.<\/jats:p>","DOI":"10.3390\/microorganisms10010101","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T03:46:27Z","timestamp":1641527187000},"page":"101","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5027-0506","authenticated-orcid":false,"given":"Marta M.","family":"Rossi","sequence":"first","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9250-5633","authenticated-orcid":false,"given":"Bruna","family":"Matturro","sequence":"additional","affiliation":[{"name":"Water Research Institute (IRSA\u2014CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4290-6735","authenticated-orcid":false,"given":"Neda","family":"Amanat","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Water Research Institute (IRSA\u2014CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8698-675X","authenticated-orcid":false,"given":"Marco","family":"Petrangeli Papini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9877","DOI":"10.1007\/s11356-015-4156-9","article-title":"Postawa, Identification of TCE and PCE sorption and biodegradation parameters in a sandy aquifer for fate and transport modelling: Batch and column studies","volume":"22","author":"Kret","year":"2015","journal-title":"Environ. 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