{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T08:10:14Z","timestamp":1773475814739,"version":"3.50.1"},"reference-count":79,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2017-0784"],"award-info":[{"award-number":["PICT-2017-0784"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2016-1993"],"award-info":[{"award-number":["PICT-2016-1993"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Rhomboids are intramembrane serine proteases highly conserved in the three domains of life. Their key roles in eukaryotes are well understood but their contribution to bacterial physiology is still poorly characterized. Here we demonstrate that Brucella abortus, the etiological agent of the zoonosis called brucellosis, encodes an active rhomboid protease capable of cleaving model heterologous substrates like Drosophila melanogaster Gurken and Providencia stuartii TatA. To address the impact of rhomboid deletion on B. abortus physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. About 50% of the B. abortus predicted proteome was identified by quantitative proteomics under two experimental conditions and 108 differentially represented proteins were detected. Membrane associated proteins that showed variations in concentration in the mutant were considered as potential rhomboid targets. This class included nitric oxide reductase subunit C NorC (Q2YJT6) and periplasmic protein LptC involved in LPS transport to the outer membrane (Q2YP16). Differences in secretory proteins were also addressed. Differentially represented proteins included a putative lytic murein transglycosylase (Q2YIT4), nitrous-oxide reductase NosZ (Q2YJW2) and high oxygen affinity Cbb3-type cytochrome c oxidase subunit (Q2YM85). Deletion of rhomboid had no obvious effect in B. abortus virulence. However, rhomboid overexpression had a negative impact on growth under static conditions, suggesting an effect on denitrification enzymes and\/or high oxygen affinity cytochrome c oxidase required for growth in low oxygen tension conditions.<\/jats:p>","DOI":"10.3390\/microorganisms10010114","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T03:46:27Z","timestamp":1641527187000},"page":"114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Brucella abortus Encodes an Active Rhomboid Protease: Proteome Response after Rhomboid Gene Deletion"],"prefix":"10.3390","volume":"10","author":[{"given":"Mar\u00eda In\u00e9s","family":"Marchesini","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas \u201cDr. Rodolfo A. Ugalde\u201d, IIB-UNSAM-CONICET, Universidad Nacional de San Mart\u00edn, San Mart\u00edn, Buenos Aires 1650, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7540-3475","authenticated-orcid":false,"given":"Ansgar","family":"Poetsch","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China"},{"name":"Center for Marine and Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology (QNLM), Qingdao 266237, China"},{"name":"Department of Plant Biochemistry, Ruhr University Bochum, 44803 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7233-5043","authenticated-orcid":false,"given":"Leticia Soledad","family":"Guidol\u00edn","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas \u201cDr. Rodolfo A. Ugalde\u201d, IIB-UNSAM-CONICET, Universidad Nacional de San Mart\u00edn, San Mart\u00edn, Buenos Aires 1650, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-4226","authenticated-orcid":false,"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas \u201cDr. Rodolfo A. Ugalde\u201d, IIB-UNSAM-CONICET, Universidad Nacional de San Mart\u00edn, San Mart\u00edn, Buenos Aires 1650, Argentina"},{"name":"Centro At\u00f3mico Ezeiza, Grupo Pecuario, Comisi\u00f3n Nacional de Energ\u00eda At\u00f3mica, Buenos Aires 1804, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0092-8674(01)00525-6","article-title":"Drosophila Rhomboid-1 defines a family of putative intramembrane serine proteases","volume":"107","author":"Urban","year":"2001","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e97909","DOI":"10.15252\/embj.201797909","article-title":"PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria","volume":"37","author":"Saita","year":"2018","journal-title":"EMBO J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1073\/pnas.1811938116","article-title":"PARL deficiency in mouse causes Complex III defects, coenzyme Q depletion, and Leigh-like syndrome","volume":"116","author":"Spinazzi","year":"2019","journal-title":"Proc. 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