{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:34:30Z","timestamp":1792010070610,"version":"4.4.0"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2017\/04249-4"],"award-info":[{"award-number":["2017\/04249-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["305872\/2013-3"],"award-info":[{"award-number":["305872\/2013-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Borrelia burgdorferi sensu lato (Bbsl) spirochetes thrive in sylvatic transmission cycles infecting vertebrates and their ticks. Rodents and ticks of the genus Ixodes are important hosts of these spirochetes globally. Although evidence suggests that Borrelia burgdorferi sensu stricto does not exist in South America, genospecies of the group (Bbsl) can be found in this region but have been poorly characterized from a genetic viewpoint, and data on their ecoepidemiology are still incipient. Aiming to detect the natural foci of Borrelia in Brazil, we targeted small mammals inhabiting seven forests fragments during a period of three years (2015\u20132018). Organs (lung) from two Oligoryzomys rodents over a total of 382 sampled mammals were positive, and we performed a molecular characterization of 10 borrelial genes to achieve a robust analysis. Phylogenetic trees inferred from 16S rRNA, flaB, ospC, and seven MLST loci (clpA, nifS, pepX, pyrG, recG, rlpB, and uvrA) support the characterization of a novel genospecies of Bbsl that we herein name \u201cCandidatus Borrelia paulista\u201d Rp42. Remarkably, \u201cCa. B. paulista\u201d is phylogenetically related to Borrelia carolinensis, a genospecies that infects Ixodes ticks and cricetid rodents in North America. A previous study performed in the same area identified Ixodes schulzei feeding on Oligoryzomys rodents. Although this tick species could be considered a probable host for this novel Borrelia sp., further research is needed to confirm this hypothesis.<\/jats:p>","DOI":"10.3390\/microorganisms10020204","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T21:01:51Z","timestamp":1642626111000},"page":"204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A Novel Genospecies of Borrelia burgdorferi Sensu Lato Associated with Cricetid Rodents in Brazil"],"prefix":"10.3390","volume":"10","author":[{"given":"B\u00e1rbara C.","family":"Weck","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carolina A.","family":"Serpa","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9675-3132","authenticated-orcid":false,"given":"Marcelo B.","family":"Labruna","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3547-6466","authenticated-orcid":false,"given":"Sebasti\u00e1n","family":"Mu\u00f1oz-Leal","sequence":"additional","affiliation":[{"name":"Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepci\u00f3n, Av. Vicente M\u00e9ndez 595, Casilla 537, Chill\u00e1n 3780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.ttbdis.2011.04.002","article-title":"Borrelia burgdorferi sensu lato complex with respect to public health","volume":"2","author":"Rudenko","year":"2011","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3836","DOI":"10.1099\/ijsem.0.000491","article-title":"Borrelia yangtzensis sp. nov., a rodent-associated species in Asia, is related to Borrelia valaisiana","volume":"65","author":"Margos","year":"2015","journal-title":"Int. J. Syst. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1007\/s00248-020-01512-x","article-title":"\u201cCandidatus Borrelia ibitipoquensis,\u201d a Borrelia valaisiana\u2013related genospecies characterized from Ixodes paranaensis in Brazil","volume":"80","author":"Ramirez","year":"2020","journal-title":"Microb. Ecol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16090","DOI":"10.1038\/nrdp.2016.90","article-title":"Lyme borreliosis","volume":"2","author":"Steere","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4878","DOI":"10.1099\/ijsem.0.001445","article-title":"Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States","volume":"66","author":"Pritt","year":"2016","journal-title":"Int. J. Syst. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Margos, G., Gofton, A., Wibberg, D., Dangel, A., Marosevic, D., Loh, S.-M., Oskam, C., and Fingerle, V. (2018). The genus Borrelia reloaded. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0208432"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.6061\/clinics\/2018\/e394","article-title":"Passage of Borrelia burgdorferi through diverse Ixodid hard ticks causes distinct diseases: Lyme borreliosis and Baggio-Yoshinari syndrome","volume":"73","author":"Miziara","year":"2018","journal-title":"Clinics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.tmaid.2018.04.005","article-title":"Lack of serological evidence for Lyme-like borreliosis in Brazil","volume":"26","author":"Oliveira","year":"2018","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Morand, S., Krasnov, B.R., and Poulin, R. (2006). Taxonomy, hosts associations, life cycles and vectorial importance of ticks parasitizing small mammals. Micromammals and Macroparasites: From Evolutionary Ecology to Management, Springer.","DOI":"10.1007\/978-4-431-36025-4"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1099\/ijs.0.021436-0","article-title":"Borrelia carolinensis sp. nov., a novel species of the Borrelia burgdorferi sensu lato complex isolated from rodents and a tick from the south-eastern USA","volume":"61","author":"Rudenko","year":"2011","journal-title":"Int. J. Syst. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1099\/ijsem.0.000897","article-title":"Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles","volume":"66","author":"Margos","year":"2016","journal-title":"Int. J. Syst. Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.1128\/IAI.01950-05","article-title":"Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection","volume":"74","author":"Tilly","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00153-10","DOI":"10.1128\/mBio.00153-10","article-title":"Evolution and distribution of the ospC gene, a transferable serotype determinant of Borrelia burgdorferi","volume":"1","author":"Barbour","year":"2010","journal-title":"mBio"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.actatropica.2016.06.030","article-title":"Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region of Argentina","volume":"162","author":"Sebastian","year":"2016","journal-title":"Acta Trop."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/1462-2920.12310","article-title":"Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the Southern Hemisphere","volume":"16","author":"Ivanova","year":"2014","journal-title":"Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.actatropica.2019.02.002","article-title":"Anaplasmataceae, Borrelia and Hepatozoon agents in ticks (Acari: Argasidae, Ixodidae) from Chile","volume":"192","author":"Lopes","year":"2019","journal-title":"Acta Trop."},{"key":"ref_17","first-page":"1","article-title":"Rodents as potential reservoirs for Borrelia spp. in northern Chile","volume":"29","author":"Thomas","year":"2020","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1016\/j.ttbdis.2017.08.003","article-title":"Borrelia burgdorferi sensu lato in Ixodes longiscutatus ticks from Brazilian Pampa","volume":"8","author":"Michel","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101805","DOI":"10.1016\/j.ttbdis.2021.101805","article-title":"Small mammals, ticks and rickettsiae in natural and human-modified landscapes: Diversity and occurrence of Brazilian spotted fever in Brazil","volume":"12","author":"Serpa","year":"2021","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_20","unstructured":"Reis, N.R., Peracchi, A.L., Pedro, W.A., and Lima, I.P. (2006). Mam\u00edferos do Brasil, SEMA."},{"key":"ref_21","unstructured":"Bonvicino, C.R. (2008). Guia dos Roedores do Brasil, com Chaves para G\u00eaneros Baseados em Caracter\u00edsticas Externas, Centro Pan-Americano de Febre Aftosa\u2014OPAS\/OMS."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00436-005-1410-y","article-title":"PCR-RFLP analysis: A promising technique for host species identification of blood meals from tsetse flies (Diptera: Glossinidae)","volume":"97","author":"Steuber","year":"2005","journal-title":"Parasitol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"883","DOI":"10.3201\/eid1705.100573","article-title":"Tick-Borne Relapsing Fever Borreliosis, Rural Senegal","volume":"17","author":"Parola","year":"2011","journal-title":"Emerg Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3851","DOI":"10.1128\/JCM.43.8.3851-3859.2005","article-title":"Phylogenetic Analysis of the Spirochetes Borrelia parkeri and Borrelia turicatae and the Potential for Tick-Borne Relapsing Fever in Florida","volume":"43","author":"Schwan","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5557","DOI":"10.1128\/JCM.41.12.5557-5562.2003","article-title":"DNA evidence of Borrelia lonestari in Amblyomma americanum (Acari: Ixodidae) removed from humans","volume":"41","author":"Stromdahl","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1099\/mic.0.26944-0","article-title":"Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe","volume":"150","author":"Bunikis","year":"2004","journal-title":"Microbiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8730","DOI":"10.1073\/pnas.0800323105","article-title":"MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi","volume":"105","author":"Margos","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.ttbdis.2018.03.008","article-title":"Isolation and molecular characterization of a relapsing fever Borrelia recovered from Ornithodoros rudis in Brazil","volume":"9","author":"Costa","year":"2018","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res. Spec. Publ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1093\/sysbio\/sys029","article-title":"MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice across a Large Model Space","volume":"61","author":"Ronquist","year":"2012","journal-title":"Syst. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Patton, J.L., Pardi\u00f1as, U.F.J., and D\u2019El\u00eda, G. (2015). Mammals of South America, University of Chicago Press.","DOI":"10.7208\/chicago\/9780226169606.001.0001"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1002\/ece3.957","article-title":"An Ixodes minor and Borrelia carolinensis enzootic cycle involving a critically endangered Mojave Desert rodent","volume":"4","author":"Foley","year":"2014","journal-title":"Ecol. Evol."},{"key":"ref_34","first-page":"109","article-title":"Prevalencia de Borrelia burgdorferi sensu lato en roedores sinantr\u00f3picos de dos comunidades rurales de Yucat\u00e1n, M\u00e9xico","volume":"36","year":"2016","journal-title":"Biomedica"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wolcott, K.A., Margos, G., Fingerle, V., and Becker, N. (2021). Host association of Borrelia burgdorferi sensu lato: A review. Ticks Tick Borne Dis., 12.","DOI":"10.1016\/j.ttbdis.2021.101766"},{"key":"ref_36","first-page":"113","article-title":"Taxonomy of pigmy rice rats genus Oligoryzomys Bangs, 1900 (Rodentia, Sigmodontinae) of the Brazilian Cerrado, with the description of two new species","volume":"63","author":"Weksler","year":"2005","journal-title":"Arquivos do Museu Nacional"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1206\/3880.1","article-title":"The Taxonomic Status of Oligoryzomys mattogrossae (Allen 1916) (Rodentia: Cricetidae: Sigmodontinae), Reservoir of Anajatuba Hantavirus","volume":"3880","author":"Weksler","year":"2017","journal-title":"Am. Mus. Novit."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1089\/vbz.2012.1102","article-title":"Borrelia burgdorferi sensu lato Infecting Ticks of the Ixodes ricinus Complex in Uruguay: First Report for the Southern Hemisphere","volume":"13","author":"Barbieri","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101349","DOI":"10.1016\/j.ttbdis.2019.101349","article-title":"Synonymy of Ixodes aragaoi with Ixodes fuscipes, and reinstatement of Ixodes spinosus (Acari: Ixodidae)","volume":"11","author":"Labruna","year":"2020","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_40","unstructured":"Nava, S., Venzal, J.M., Gonz\u00e1lez-Acu\u00f1a, D., Martins, T.F., and Guglielmone, A.A. (2017). Ticks of the Southern Cone of America: Diagnosis, Distribution and Hosts with Taxonomy, Ecology and Sanitary Importance, Elsevier."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Guglielmone, A., and Robbins, R. (2018). Tick Species Found Feeding on Humans: A Global Overview. Hard Ticks (Acari: Ixodida: Ixodidae) Parasitizing Humans, Springer.","DOI":"10.1007\/978-3-319-95552-0"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/2\/204\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:03:43Z","timestamp":1760133823000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/2\/204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,19]]},"references-count":41,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["microorganisms10020204"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10020204","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,19]]}}}