{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:28:04Z","timestamp":1790681284945,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002738","name":"Assistance Publique - H\u00f4pitaux de Paris","doi-asserted-by":"publisher","award":["Bic\u00eatre"],"award-info":[{"award-number":["Bic\u00eatre"]}],"id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007241","name":"University of Paris-Saclay","doi-asserted-by":"publisher","award":["1184"],"award-info":[{"award-number":["1184"]}],"id":[{"id":"10.13039\/501100007241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-17-ASTR-0018-03"],"award-info":[{"award-number":["ANR-17-ASTR-0018-03"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DIM maladie infectieuse","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Since the first description of OXA-48, more than forty variants have been recovered from Enterobacterales isolates. Whereas some OXA-48-related enzymes have been reported as conferring similar resistance patterns, namely, the hydrolysis of carbapenems and penicillins with very weak or almost no activity against expanded-spectrum cephalosporins, some have reduced carbapenem and temocillin hydrolysis, and others hydrolyze expanded-spectrum cephalosporins and carbapenems only marginally. With such drastic differences in the hydrolytic profile, especially of carbapenems, it becomes urgent to establish hydrolytic cutoffs in order to determine when an OXA-48-like enzyme may be considered as a carbapenemase or not. With this aim, the coefficient of activity for imipenem (kcat\/Km) was determined for a total of 30 enzymes, including OXA-48, OXA-48-like natural variants, and OXA-48 synthetic mutants. In addition, six different methods for the detection of carbapenemase-producers were performed. The coefficients of activity for imipenem for all the different enzymes went from 550 mM\u22121\u00b7s\u22121 to 0.02 mM\u22121\u00b7s\u22121. In order to match the coefficient of activity results with the biochemical confirmatory tests, we suggest the value of 0.27 mM\u22121\u00b7s\u22121 as the cutoff above which an OXA-48 variant may be considered a carbapenem-hydrolyzing enzyme.<\/jats:p>","DOI":"10.3390\/microorganisms10020258","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T20:40:18Z","timestamp":1643143218000},"page":"258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["To Be or Not to Be an OXA-48 Carbapenemase"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6740-5043","authenticated-orcid":false,"given":"Laura","family":"Dabos","sequence":"first","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5766-3831","authenticated-orcid":false,"given":"Saoussen","family":"Oueslati","sequence":"additional","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique-H\u00f4pitaux de Paris, AP-HP Paris-Saclay, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bernabeu","sequence":"additional","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique-H\u00f4pitaux de Paris, AP-HP Paris-Saclay, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2307-3232","authenticated-orcid":false,"given":"R\u00e9my A.","family":"Bonnin","sequence":"additional","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique-H\u00f4pitaux de Paris, AP-HP Paris-Saclay, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique-H\u00f4pitaux de Paris, AP-HP Paris-Saclay, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9937-9572","authenticated-orcid":false,"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Team \u201cResist\u201d UMR1184 \u201cImmunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB)\u201d, INSERM, Universit\u00e9 Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique-H\u00f4pitaux de Paris, AP-HP Paris-Saclay, Bic\u00eatre Hospital, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ramirez, M.S., Bonomo, R.A., and Tolmasky, M.E. 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