{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:26:09Z","timestamp":1780449969061,"version":"3.54.1"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Users of prosthetic devices face the accumulation of potentially drug-resistant pathogenic bacteria on the skin\/prosthesis interface. In this study, we took surface swabs of the skin\/prosthesis interface of eleven disabled athletes to identify microorganisms present. In addition to determining their antimicrobial resistance profile, we assessed their sensitivity to Manuka honey and Garlic extract (allicin). Eleven volunteers were directed to swab the skin at the skin\/prosthesis interface. After initial isolation of microorganisms, we employed the following general microbiological methods: Gram stain, Catalase test, Oxidase test, lactose fermenting capability, haemolytic capability, Staphaurex, mannitol fermenting capability, Streptex; API Staph, 20E, Candida, and BBL crystal identification system tests. Once identified, isolates were analysed for their sensitivity to penicillin, erythromycin, ampicillin, vancomycin, ceftazidime, ciprofloxacin, gentamicin, and colistin-sulphate. Isolates were also analysed for their sensitivity to allicin (Garlic Extract (GE)) and Manuka honey (Medihoney\u2122) (MH). Eleven isolates were identified: Bacillus cereus, Staphylococcus haemolyticus, Staphylococcus aureus, Micrococcus luteus, Pseudomonas oryzihabitans, Micrococcus spp., Bacillus subtilis, Group D Streptococcus, Pantoea spp., Enterobacter cloacae, and Bergeyella zoohelcum. All isolates were resistant to 1 unit of penicillin and 10 \u03bcg of ampicillin. Bergeyella zoohelcum was observed to have the widest range of resistance with observed resistance against five of the eight antimicrobials employed in this study. This study highlights the prevalence of uncommon drug-resistant microorganisms on the skin within a vulnerable population, highlighting the potential for MH or GE intervention.<\/jats:p>","DOI":"10.3390\/microorganisms10020299","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T04:49:51Z","timestamp":1643258991000},"page":"299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Identification of Multidrug-Resistant Microorganisms including Bergeyella zoohelcum Acquired from the Skin\/Prosthetic Interface of Amputees and Their Susceptibility to Medihoney\u2122 and Garlic Extract (Allicin)"],"prefix":"10.3390","volume":"10","author":[{"given":"Ruby","family":"Harsent","sequence":"first","affiliation":[{"name":"Llandaff Campus, School of Sport and Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Macleod","sequence":"additional","affiliation":[{"name":"Zero2Five Food Industry Centre, Llandaff Campus, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard S.","family":"Rowlands","sequence":"additional","affiliation":[{"name":"Llandaff Campus, School of Sport and Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Cyncoed Campus, School of Sport and Health Sciences, Cardiff Metropolitan University, Cyncoed Road, Cardiff CF23 6XD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Rushmere","sequence":"additional","affiliation":[{"name":"Llandaff Campus, School of Sport and Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-5563","authenticated-orcid":false,"given":"James","family":"Blaxland","sequence":"additional","affiliation":[{"name":"Zero2Five Food Industry Centre, Llandaff Campus, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,26]]},"reference":[{"key":"ref_1","unstructured":"Diabetes UK (2021, August 09). 26,378 Diabetes-Related Lower Limb Amputations in the Last Three Years. Available online: https:\/\/www.diabetes.org.uk\/about_us\/news\/lower-limb-amputations."},{"key":"ref_2","unstructured":"World Health Organization (WHO) (2020, September 07). Guidelines for Training Personnel in Developing countries for Prosthetics and Orthotics Services. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/43127."},{"key":"ref_3","first-page":"A2010","article-title":"The endo-exo prosthesis for patients with a problematic amputation stump","volume":"154","year":"2010","journal-title":"Ned. Tijdschr. Geneeskd."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1016\/j.apmr.2012.12.007","article-title":"Satisfaction and Problems Experienced with Transfemoral Suspension Systems: A Comparison Between Common Suction Socket and Seal-In Liner","volume":"94","author":"Gholizadeh","year":"2013","journal-title":"Arch. Phys. Med. Rehabil."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.aott.2017.07.001","article-title":"Factors affecting lifespan following below-knee amputation in diabetic patients","volume":"51","author":"Beyaz","year":"2017","journal-title":"Acta Orthop. Traumatol. Turc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1177\/0141076816688346","article-title":"Prevention and treatment of diabetic foot ulcers","volume":"110","author":"Lim","year":"2017","journal-title":"J. R. Soc. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Weledji, E.P., and Fokam, P. (2014). Treatment of the diabetic foot\u2014To amputate or not?. BMC Surg., 14.","DOI":"10.1186\/1471-2482-14-83"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1080\/10400435.2016.1225850","article-title":"Temperature measurement and control system for transtibial prostheses: Functional evaluation","volume":"30","author":"Ghoseiri","year":"2018","journal-title":"Assist. Technol."},{"key":"ref_9","unstructured":"Cooper, S. (2012). Bacterial Growth and Division: Biochemistry and Regulation of Prokaryotic and Eukaryotic Division Cycles, Elsevier."},{"key":"ref_10","unstructured":"OPEDGE.COM (2020, June 25). Responses to Amputee Odor Question. Available online: https:\/\/opedge.com\/OANDPL\/ViewMessage\/6539CC54-6905-428D-9A4B-982C9E674B37."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"204","DOI":"10.2310\/6620.2006.06014","article-title":"Triclosan","volume":"17","author":"Campbell","year":"2006","journal-title":"Dermatitis"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e02312","DOI":"10.1128\/AAC.02312-18","article-title":"The Widely Used Antimicrobial Triclosan Induces High Levels of Antibiotic Tolerance In Vitro and Reduces Antibiotic Efficacy up to 100-Fold In Vivo","volume":"63","author":"Westfall","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"780","DOI":"10.3389\/fmicb.2014.00780","article-title":"The impact of triclosan on the spread of antibiotic resistance in the environment","volume":"5","author":"Carey","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"M2228-40","DOI":"10.1111\/1750-3841.12993","article-title":"Biomedical Activity and Related Volatile Compounds of Thai Honeys from 3 Different Honeybee Species","volume":"80","author":"Pattamayutanon","year":"2015","journal-title":"J. Food Sci."},{"key":"ref_15","unstructured":"Comvita\u00ae UK (2020, October 26). The Immense Benefits of Medihoney. Available online: https:\/\/www.comvita.co.uk\/ingredients\/manuka-honey\/the-science-behind-manuka-honey.list?gclid=EAIaIQobChMI4peeyabP9QIVFIfVCh2JywcBEAAYASACEgIUwPD_BwE&gclsrc=aw.ds."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/j.1365-2648.2008.04923.x","article-title":"Standardized antibacterial honey (MedihoneyTM) with standard therapy in wound care: Randomized clinical trial","volume":"65","author":"Robson","year":"2009","journal-title":"J. Adv. Nurs."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/sc.2011.87","article-title":"Use of Medihoney as a non-surgical therapy for chronic pressure ulcers in patients with spinal cord injury","volume":"50","author":"Biglari","year":"2012","journal-title":"Spinal Cord."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1097\/DSS.0000000000001382","article-title":"Mechanism of Action of Topical Garlic on Wound Healing","volume":"44","author":"Alhashim","year":"2018","journal-title":"Dermatol. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Reiter, J., Levina, N., van der Linden, M., Gruhlke, M., Martin, C., and Slusarenko, A.J. (2017). Diallylthiosulfinate (Allicin), a Volatile Antimicrobial from Garlic (Allium sativum), Kills Human Lung Pathogenic Bacteria, Including MDR Strains, as a Vapor. Molecules, 22.","DOI":"10.3390\/molecules22101711"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.3389\/fimmu.2017.01678","article-title":"Protective Microbiota: From Localized to Long-Reaching Co-Immunity","volume":"8","author":"Chiu","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1111\/j.1365-2133.2008.08437.x","article-title":"Skin microbiota: A source of disease or defence?","volume":"158","author":"Cogen","year":"2008","journal-title":"Br. J. Dermatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/CMR.00134-14","article-title":"Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management","volume":"28","author":"Tong","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_23","unstructured":"Ferretti, J.J., Stevens, D.L., and Fischetti, V.A. (2016). Impetigo, Erysipelas and Cellulitis. Streptococcus pyogenes: Basic Biology to Clinical Manifestations, University of Oklahoma Health Sciences Center."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"K\u00fchbacher, A., Burger-Kentischer, A., and Rupp, S. (2017). Interaction of Candida Species with the Skin. Microorganisms, 5.","DOI":"10.3390\/microorganisms5020032"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4103\/0974-777X.145253","article-title":"Soft Tissue and Wound Infections Due to Enterococcus spp. Among Hospitalized Trauma Patients in a Developing Country","volume":"6","author":"Rajkumari","year":"2014","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_26","unstructured":"George, E.K., De Jesus, O., and Vivekanandan, R. (2020). Clostridium Tetani, StatPearls Publishing."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/CMR.00073-09","article-title":"Bacillus cereus, a Volatile Human Pathogen","volume":"23","author":"Bottone","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4161\/viru.22913","article-title":"Candida albicans pathogenicity mechanisms","volume":"4","author":"Mayer","year":"2013","journal-title":"Virulence"},{"key":"ref_29","unstructured":"Public Health England (2019, May 30). UK Standards for Microbiology Investigations: Introduction to the Preliminary Identification of Med-ically Important Bacteria and Fungi from Culture Standards Unit, Available online: https:\/\/assets.publishing.service.gov.uk\/government\/uploads\/system\/uploads\/attachment_data\/file\/1020471\/ID_1i3.pdf."},{"key":"ref_30","unstructured":"Public Health England (2019, May 30). UK Standards for Microbiology Investigations: Staining Procedures, Available online: https:\/\/assets.publishing.service.gov.uk\/government\/uploads\/system\/uploads\/attachment_data\/file\/802769\/TP_39i3.pdf."},{"key":"ref_31","unstructured":"Public Health England (2020, May 30). UK Standards for Microbiology Investigations: Catalase Test 2019, Available online: https:\/\/www.gov.uk\/government\/publications\/smi-tp-8-catalase-test."},{"key":"ref_32","unstructured":"Public Health England (2020, July 20). UK Standards for Microbiology Investigations: Oxidase Test, Available online: https:\/\/www.gov.uk\/government\/publications\/smi-tp-26-oxidase-test."},{"key":"ref_33","unstructured":"Public Health England (2020, July 20). UK Standards for Microbiology Investigations: Inoculation of Culture Media for Bacteriology, Available online: https:\/\/www.gov.uk\/government\/publications\/smi-q-5-inoculation-of-culture-media-for-bacteriology."},{"key":"ref_34","unstructured":"Jung, B., and Hoilat, G.J. (2020). MacConkey Medium, StatPearls Publishing."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1128\/aem.60.4.1179-1183.1994","article-title":"Blood agar to detect virulence factors in tap water heterotrophic bacteria","volume":"60","author":"Payment","year":"1994","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/JCM.39.1.86-89.2001","article-title":"International Multicenter Evaluation of Latex Agglutination Tests for Identification of Staphylococcus aureus","volume":"39","author":"Bes","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","unstructured":"Thermo Fisher Scientific (2021, August 20). Remel. Staphaurex. Available online: https:\/\/www.fishersci.co.uk\/shop\/products\/remel-staphaurex-diagnostic-tests\/p-4529287."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.micpath.2017.02.034","article-title":"Comparison of growth on mannitol salt agar, matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry, VITEK\u00ae 2 with partial sequencing of 16S rRNA gene for identification of coagulase-negative staphylococci","volume":"105","author":"Ayeni","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0732-8893(84)90006-3","article-title":"Clinical evaluation of the improved streptex method for grouping streptococci","volume":"2","author":"Martin","year":"1984","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_40","unstructured":"Thermo Fisher Scientific (2020, July 12). Remel. Streptex Rapid Remel Europe. Available online: https:\/\/www.thermofisher.com\/order\/catalog\/product\/R30950555."},{"key":"ref_41","unstructured":"BioM\u00e9rieux (2020, July 12). API Staph 2013. Available online: https:\/\/www.mediray.co.nz\/media\/15784\/om_biomerieux_test-kits_ot-20500_package_insert-20500.pdf."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1128\/jcm.16.3.509-516.1982","article-title":"Identification of Staphylococcus species with the API STAPH-IDENT system","volume":"16","author":"Kloos","year":"1982","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","unstructured":"BioM\u00e9rieux (2020, July 14). API 20 E 2002. Available online: http:\/\/biomanufacturing.org\/uploads\/files\/587872707301898351-api20einstructions.pdf."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1136\/jcp.31.1.22","article-title":"Identification of Enterobacteriaceae by the API 20E system","volume":"31","author":"Holmes","year":"1978","journal-title":"J. Clin. Pathol."},{"key":"ref_45","unstructured":"BioM\u00e9rieux (2021, August 03). API Candida 2011. Available online: https:\/\/www.mediray.co.nz\/media\/15780\/om_biomerieux_test-kits_ot-10500_package_insert_-10500.pdf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1128\/JCM.37.3.821-823.1999","article-title":"Comparison of the API Candida System with the AUXACOLOR System for Identification of Common Yeast Pathogens","volume":"37","author":"Campbell","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_47","unstructured":"BD Advancing the World of Health (2020, July 20). BBL CrystalTM Identification Systems Gram-Positive ID Kit 2002. Available online: https:\/\/legacy.bd.com\/ds\/technicalCenter\/clsi\/clsi-Crysgp.pdf."},{"key":"ref_48","unstructured":"BD Advancing the World of Health (2020, July 20). BBL CrystalTM Identification Systems Enteric\/Nonfermenter ID Kit 2001. Available online: https:\/\/legacy.bd.com\/ds\/technicalCenter\/clsi\/clsi-CrysE_nf.pdf."},{"key":"ref_49","unstructured":"Hudzicki, J. (2009). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol, American Society for Microbiology."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"197","DOI":"10.5604\/12321966.1203878","article-title":"Pantoea agglomerans: A mysterious bacterium of evil and good. Part III. Deleterious effects: Infections of humans, animals and plants","volume":"23","author":"Dutkiewicz","year":"2016","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"991","DOI":"10.3892\/etm.2016.3353","article-title":"Risk factors and the clinical and surgical features of fungal prosthetic joint infections: A retrospective analysis of eight cases","volume":"12","author":"Geng","year":"2016","journal-title":"Exp. Ther. Med."},{"key":"ref_52","unstructured":"O&P Virtual Library (2020, November 04). 26: Skin Problems of the Amputee. Available online: http:\/\/www.oandplibrary.org\/alp\/chap26-01.asp."},{"key":"ref_53","first-page":"6","article-title":"Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment","volume":"18","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4103\/jdds.jdds_43_18","article-title":"Pantoea agglomerans cutaneous infection","volume":"23","author":"Okwundu","year":"2019","journal-title":"J. Dermatol. Dermatol. Surg."},{"key":"ref_55","first-page":"820","article-title":"Pseudomonas oryzihabitans: An unusual cause of skin and soft tissue infection","volume":"47","author":"Tena","year":"2015","journal-title":"Infect. Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1128\/JCM.05563-11","article-title":"Staphylococcus haemolyticus as an Important Hospital Pathogen and Carrier of Methicillin Resistance Genes","volume":"50","author":"Barros","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_57","unstructured":"Turnbull, P. (1996). Medical Microbiology, University of Texas Medical Branch at Galveston. [4th ed.]. NCBI Bookshelf."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1128\/CMR.14.2.244-269.2001","article-title":"Wound Microbiology and Associated Approaches to Wound Management","volume":"14","author":"Bowler","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_59","unstructured":"Ellis, G.P., Luscombe, D.K., and Oxford, A.W. (1998). 4-Mechanisms of bacterial resistance to antibiotics and biocides. Progress in Medicinal Chemistry, Elsevier."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0929-6646(07)60008-4","article-title":"Cellulitis and Bacteremia Caused by Bergeyella zoohelicum","volume":"106","author":"Lin","year":"2007","journal-title":"J. Formos. Med. Assoc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1111\/zph.12644","article-title":"Bergeyella zoohelicum isolated from oral cavities of therapy dogs","volume":"66","author":"Muramatsu","year":"2019","journal-title":"Zoonoses Public Health"},{"key":"ref_62","first-page":"e4494","article-title":"Bergeyella zoohelicum Bacteremia from Therapy Dog Kisses","volume":"11","author":"Sharma","year":"2019","journal-title":"Cureus"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1128\/AAC.44.10.2747-2751.2000","article-title":"Comparative In Vitro Activities of GAR-936 against Aerobic and Anaerobic Animal and Human Bite Wound Pathogens","volume":"44","author":"Goldstein","year":"2000","journal-title":"Anitmicrobial. Agents Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"569","DOI":"10.3389\/fmicb.2016.00569","article-title":"Therapeutic Manuka Honey: No Longer So Alternative","volume":"7","author":"Carter","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1128\/JCM.42.1.290-293.2004","article-title":"Isolation of a Fastidious bergeyella Species Associated with Cellulitis after a Cat Bite and a Phylogenetic Comparison with Bergeyella zoohelicum Strains","volume":"42","author":"Shukla","year":"2004","journal-title":"J. Clin. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/2\/299\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:08:33Z","timestamp":1760134113000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/2\/299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,26]]},"references-count":65,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["microorganisms10020299"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10020299","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,26]]}}}