{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:23:49Z","timestamp":1789043029724,"version":"build-2803163510"},"reference-count":101,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010751","name":"Fondecyt","doi-asserted-by":"publisher","award":["1200756, 11130117"],"award-info":[{"award-number":["1200756, 11130117"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ANID PIA Ring","award":["ACT172128"],"award-info":[{"award-number":["ACT172128"]}]},{"DOI":"10.13039\/100010066","name":"USM","doi-asserted-by":"publisher","award":["PI_M_2020_43"],"award-info":[{"award-number":["PI_M_2020_43"]}],"id":[{"id":"10.13039\/100010066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Heavy metal co-contamination in crude oil-polluted environments may inhibit microbial bioremediation of hydrocarbons. The model heavy metal-resistant bacterium Cupriavidus metallidurans CH34 possesses cadmium and mercury resistance, as well as genes related to the catabolism of hazardous BTEX aromatic hydrocarbons. The aims of this study were to analyze the aromatic catabolic potential of C. metallidurans CH34 and to determine the functionality of the predicted benzene catabolic pathway and the influence of cadmium and mercury on benzene degradation. Three chromosome-encoded bacterial multicomponent monooxygenases (BMMs) are involved in benzene catabolic pathways. Growth assessment, intermediates identification, and gene expression analysis indicate the functionality of the benzene catabolic pathway. Strain CH34 degraded benzene via phenol and 2-hydroxymuconic semialdehyde. Transcriptional analyses revealed a transition from the expression of catechol 2,3-dioxygenase (tomB) in the early exponential phase to catechol 1,2-dioxygenase (catA1 and catA2) in the late exponential phase. The minimum inhibitory concentration to Hg (II) and Cd (II) was significantly lower in the presence of benzene, demonstrating the effect of co-contamination on bacterial growth. Notably, this study showed that C. metallidurans CH34 degraded benzene in the presence of Hg (II) or Cd (II).<\/jats:p>","DOI":"10.3390\/microorganisms10020484","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:47:45Z","timestamp":1645476465000},"page":"484","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Cupriavidus metallidurans CH34 Possesses Aromatic Catabolic Versatility and Degrades Benzene in the Presence of Mercury and Cadmium"],"prefix":"10.3390","volume":"10","author":[{"given":"Pablo","family":"Alviz-Gazitua","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Biotecnolog\u00eda Ambiental, Departamento de Qu\u00edmica & Centro de Biotecnolog\u00eda, Universidad T\u00e9cnica Federico Santa Mar\u00eda, Avenida Espa\u00f1a 1680, Valpara\u00edso 2390123, Chile"},{"name":"Departamento de Ciencias Biol\u00f3gicas y Biodiversidad, Universidad de los Lagos, Osorno 5311890, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0077-9483","authenticated-orcid":false,"given":"Roberto E.","family":"Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Biotecnolog\u00eda Ambiental, Departamento de Qu\u00edmica & Centro de Biotecnolog\u00eda, Universidad T\u00e9cnica Federico Santa Mar\u00eda, Avenida Espa\u00f1a 1680, Valpara\u00edso 2390123, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8937-4778","authenticated-orcid":false,"given":"Felipe A.","family":"Millacura","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Biotecnolog\u00eda Ambiental, Departamento de Qu\u00edmica & Centro de Biotecnolog\u00eda, Universidad T\u00e9cnica Federico Santa Mar\u00eda, Avenida Espa\u00f1a 1680, Valpara\u00edso 2390123, Chile"},{"name":"School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Biotecnolog\u00eda Ambiental, Departamento de Qu\u00edmica & Centro de Biotecnolog\u00eda, Universidad T\u00e9cnica Federico Santa Mar\u00eda, Avenida Espa\u00f1a 1680, Valpara\u00edso 2390123, Chile"},{"name":"Centro Regional de Estudios en Alimentos Saludables (CREAS), Avenida Universidad 330, Curauma, Valpara\u00edso 2373223, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6935-8711","authenticated-orcid":false,"given":"Luis A.","family":"Rojas","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Facultad de Ciencias, Universidad Cat\u00f3lica del Norte, Avenida Angamos 610, Antofagasta 1270709, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3925-1996","authenticated-orcid":false,"given":"Michael","family":"Seeger","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Biotecnolog\u00eda Ambiental, Departamento de Qu\u00edmica & Centro de Biotecnolog\u00eda, Universidad T\u00e9cnica Federico Santa Mar\u00eda, Avenida Espa\u00f1a 1680, Valpara\u00edso 2390123, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/nrmicro2652","article-title":"Microbial degradation of aromatic compounds- From one strategy to four","volume":"9","author":"Fuchs","year":"2011","journal-title":"Nat. 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