{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:19:59Z","timestamp":1781108399892,"version":"3.54.1"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Urinary tract infections caused by extended-spectrum \u03b2-lactamase Escherichia coli (ESBL-EC) are increasing worldwide and are a current concern because treatment options are often limited. This study investigated antimicrobial susceptibility, antimicrobial resistance genes (ARGs), and the biological diversity of urinary ESBL-EC isolates at Cerdanya Hospital, a European cross-border hospital that combines French and Spanish healthcare models. Bacterial identification and susceptibility were determined using the Microscan WalkAway\u00ae system and ESBL production was examined by the double-disk synergy method. Isolates were sequenced using the Ion S5\u2122 next-generation sequencing system, with the whole-genome sequences then assembled using SPADEs software and analyzed using PubMLST, ResFinder, FimTyper, PlasmidFinder, and VirulenceFinder. A phylogenetic analysis was performed by constructing an assembly-based core-SNV alignment, followed by a phylogenetic tree constructed using Parsnp from the Harvest suite. All isolates studied were multidrug-resistant and could be classified into 19 different sequence types characterized by a high genetic diversity. The most prevalent ESBL-enzymes were CTX-M-14 and CTX-M-15. High-risk international clones (ST131, ST10, and ST405) were also identified. The results demonstrated the absence of a single predominant clone of ESBL-MDR-EC at Cerdanya Hospital.<\/jats:p>","DOI":"10.3390\/microorganisms10030488","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T22:34:30Z","timestamp":1645569270000},"page":"488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Descriptive Analysis of Urinary ESBL-Producing-Escherichia coli in Cerdanya Hospital"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5513-7010","authenticated-orcid":false,"given":"Lorena Patr\u00edcia","family":"Gaviria","sequence":"first","affiliation":[{"name":"Internal Medicine Service, Hospital Transfronterer de Cerdanya, Puigcerd\u00e0, 17520 Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Montsant","sequence":"additional","affiliation":[{"name":"Internal Medicine Service, Hospital Transfronterer de Cerdanya, Puigcerd\u00e0, 17520 Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Azuaje","sequence":"additional","affiliation":[{"name":"Internal Medicine Service, Hospital Transfronterer de Cerdanya, Puigcerd\u00e0, 17520 Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8326-1307","authenticated-orcid":false,"given":"Aida","family":"Gonz\u00e1lez-D\u00edaz","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Universitari de Bellvitge, IDIBELL, Hospitalet de Llobregat, 08907 Barcelona, Spain"},{"name":"Ciber de Enfermedades Respiratorias (CIBERes), ISCIII, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9873-5459","authenticated-orcid":false,"given":"Juan P.","family":"Horcajada","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Hospital del Mar, Institut Hospital del Mar d\u2019Investigacions M\u00e8diques (IMIM), 08003 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5396-1521","authenticated-orcid":false,"given":"Enric","family":"Lim\u00f3n","sequence":"additional","affiliation":[{"name":"Programa VINCat, Institut Catal\u00e0 d\u2019Oncologia, Hospitalet de Llobregat, 08907 Barcelona, Spain"},{"name":"Department of Public Health, Mental Health and Perinatal Nursing, US of Nursing, University of Barcelona, Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2073-3386","authenticated-orcid":false,"given":"Miguel","family":"Vi\u00f1as","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & Experimental Therapeutics, Bellvitge Institute for Biomedical Research (IDIBELL), Medical School, University of Barcelona, Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1014-8936","authenticated-orcid":false,"given":"Paula","family":"Espinal","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & Experimental Therapeutics, Bellvitge Institute for Biomedical Research (IDIBELL), Medical School, University of Barcelona, Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3561-9765","authenticated-orcid":false,"given":"Ester","family":"Fust\u00e9","sequence":"additional","affiliation":[{"name":"Department of Public Health, Mental Health and Perinatal Nursing, US of Nursing, University of Barcelona, Hospitalet de Llobregat, 08907 Barcelona, Spain"},{"name":"Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & Experimental Therapeutics, Bellvitge Institute for Biomedical Research (IDIBELL), Medical School, University of Barcelona, Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"dlab092","DOI":"10.1093\/jacamr\/dlab092","article-title":"Extended-spectrum \u03b2-lactamases: An update on their characteristics, epidemiology and detection","volume":"3","author":"Castanheira","year":"2021","journal-title":"JAC-Antimicrob. 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